year 12, Issue 1 (March - April 2018)                   Iran J Med Microbiol 2018, 12(1): 33-42 | Back to browse issues page

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Alinezhad S, Irajian G, Ghaemmaghami S S. Evaluation of Fungal and Aflatoxin Contamination in Factory-made Feed in Rainbow trout Farms in Damghan City. Iran J Med Microbiol 2018; 12 (1) :33-42
1- Institiute of Technical and Vocational Higher Education, Agriculture Jihad, AREEO, Tehran, Iran ,
2- Rasool Akram Agricultural & Natural Resource Education Center, Institiute of Technical and Vocational Higher Education, Agriculture Jihad, AREEO, Tehran, Iran
3- Institiute of Technical and Vocational Higher Education, Agriculture Jihad, AREEO, Tehran, Iran
Abstract:   (5662 Views)
Background and Aims: Aquatic feed consists of different components; the undesirable quality of each of them affects the quality of the meat produced. Fungi are agents of contamination and poison production in food. Aflatoxins are of particular importance among the toxins of fungi. This poison has the ability to transfer through feeds to fish and then humans. The aim of this study was to determine the amount of aflatoxin in the feed and to identify the fungal flora.
Materials and Methods: In this study, 8 rainbow trout farms in Damghan city were evaluated for the assessment of fungal flora and total aflatoxin levels. 500 g sample of feed (pellet) was randomly prepared from the storeroom of the 8 fish farms. Fungi of Aspergillus Flavous and also fungal flora were isolated and identified by culture and slide culture method. Total aflatoxin measurement was performed using ELISA method.
Results: A total of 11 genera (Aspergillus, Fusarium, Penicillium, Mucor, Scopulariopsis, Chrysosporium, Rhizopus, Absidia, Ulocladium, Alternaria, Pseudallescheria) were identified. The genera of Rhizopus and Aspergillus were the highest with 20.38% and 18.47% respectively. Among Aspergillus species, Aspergillus flavus was the highest with 48.44% and A. ochraceus had the lowest incidence of 10.34%. A. niger was also reported with 24.74% of the reported cases. The lowest frequency was found in the genus Chrysosporium with 1.28%. The average of total aflatoxin concentration was 5.36 ppb in the range of 2.03-11.64.
Conclusions: The aflatoxin content in all samples from Rainbow trout consumption was less than the permitted level (20 ppb).
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Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2018/02/8 | Accepted: 2018/03/5 | ePublished: 2018/05/14

1. Boziaris, IS. Seafood Processing Technology, Quality and Safety. West Sussex: Wiley Blackwell; 2014. [DOI]
2. Motalebi AA, Razavilar V. High performance liquid chromatographic determination of aflatoxins in Iranian shrimp. Pajouhesh & Sazandegi. 2004;61:65-70.
3. Food and Agriculture Organization of the United Nation. The State of World Fisheries and Aquaculture. Roma: FAO; 2014.
4. [homepage on the Internt]. Rome: An Overview of Recently Published Global Aquaculture Statistics./ [updated 2015]. Available from: [Article]
5. [homepage on the Internt]. Rome: FAO Global Capture Production database updated to 2015 Summary information Fisheries and Aquaculture Department./ [updated 2015]. Available from: [Article]
6. Statistical yearbook of Iran fisheries, 2003-2013, Iran fisheries organization. Management and resources development deputy, the office of management and budget, Department of statistics, 2014.
7. Statistical yearbook of Iran fisheries, 2013-2014, Iran fisheries organization. Management and resources development deputy, the office of management and budget, Department of statistics, 2015.
8. Ebrahimzadeh Mousavi HA, Hoosseinifard SM, Khosravi AR, Soltani M, Yosefian M. Evaluation of antifungal activity of Sulangan, Cumin, Antarctic and Garlic oils of Fusarium Solana isolated from ornamental aquarium fish. J Vet Res. 2007;62(3):163-8.
9. Ebrahimi Mohammadi K, Razavilar V. A survey on prevalence rate of Aflatoxigenic species of Aspergillus and residues of aflatoxins by ELISA method in Rainbow Trout feeds in Tehran and West Azarbayjan provinces – Iran. J of comparative pathobiology. 2011;8(1):385-94.
10. Hardy RW, Castro E. Characteristics of the Chilean salmonid feed industry. Aquaculture. 1994;124:307-20 [DOI]
11. Moren M, Suontama J, Hemre GI, Karlsen Ø, Olsen RE, Mundheim H, Julshamn K. Element concentrations in meals from krill and amphipods, possible alternative protein sources in complete diets for farmed fish. Aquaculture. 2006;261:174-81. [DOI]
12. NRC. Nutrient Requirement of Fish and Shrimp. Committee on animal nutrition. Board on agriculture. National research council. Washington DC: National Academy Press; 2011.
13. Allameh A, Razzaghi-Abyaneh M. Mycotoxins. 1st ed. Tehran: Emam Hossein University; 2001.
14. Razzaghi-Abyaneh M, Pilehvar-Soltanahmadi Y, Shams-Ghahfarokhi M, Alinezhad S. Aflatoxins, Public Health and Agricultural Significance. Tehran: ITVHE; 2011. [PubMed]
15. Hedayati MT. A survey on wheat samples for mycotoxin zearalenone from Mazandaran Province 2002. J Mazandaran Univ Med Sci. 2005;15(49):89-94.
16. Ebrahimzadeh A, Mohammadzadeh F, Salimi A. Prevalence of fungal contamination of flours in Zahedan Bakeries in 2013. Med J Mashad Univ Med Sci. 2016;57(5):705-10.
17. Leong YH, Ismail N, Latif AA, Ahmad R. Short Communication: Aflatoxin occurrence in nuts and commertial nutty products in Malaysia. Food Control. 2010;21:334-8. [DOI]
18. Magan N, Olsen M. Mycotoxins in Food Detection and Control. Cambridge: Woodhead Publishing Limited; 2004.
19. Royes JB, Yanong RPE. Molds in fish and aflatoxicosis. 2002, available at downloaded in 02/09/2011.
20. Hendricks JD. 1994 Carcinogenicity of aflatoxins in non-mammalian organisms. In: The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance. San Diego; Academic Press; 1994.103–36. [DOI]
21. Sahoo PK, Mukherjee SC, Jain AK, Mukherjee A. Histopathological and electron microscopic studies of gills and opisthonephros of Rohu, Labeo rohita to acute and subchronic Aflatoxin B1 toxicity. Asian Fish Sci. 2003;16:257–68.
22. Alinezhad S, Tolouee M, Kamalzadeh A, Motalebi A A, Nazeri M, Yasemi M, Shams-Ghahfarokhi M, Tolouei R, Razzaghi-Abyaneh M. Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi. IFSRI. 2011;10(3):363-74.
23. Alinezhad S, Razzaghi-Abyaneh M, Ghaemmagami SS, Khajehrahimi AE, Rahanandeh M, Saberi SR. Fungal contamination in handmade and factory-made feed of Rainbow Trout (Oncorhincus mykiss). Pajouhesh & Sazandegi. 2013;100:35-45.
24. Motalebi AA, Ardalani K, Jamili S. Effect of temperature on the produced aflatoxins in the rainbow trout feed in West Azerbaijan province. JFAS. 2003;3:392-7.
25. Ghaemmaghami SS, Modirsaneii M, Khosravi AR, Razzaghi-Abyaneh M. Study on mycoflora of poultry feed ingredients and finished feed in Iran. Iranian journal of microbiology. 2016;8(1):47. [PubMed]
26. Mayahi M, Razi Jalali M, Salamat N. Isolation of Aspergillus spp and determination of aflatoxin level in fish meal, maize and soya meal. Shahid Chamran University Journal of Science. 2007;17:95-105.
27. Raeisi M, Rahimi A, Fadaeifard F, Ansari M. Determination of aflatoxin B1 and total in cultured rainbow trout feed in Chaharmahal & Bakhtyari Province. Vet J of Islamic Azad University Garmsar Branch. 2007;3(3):149-52.
28. Jalilpour J, Vahabzadeh H, Sepahdari A, Pajand Z. Effect of toxicity contaminated food by Aflatoxin B1 on growth and some liver enzymes of starred sturgeon (Acipenser stellatus) juveniles. J Aquac Res Dev. 2014;7(4):1-11.
29. , aflatoxins and aflatoxicosis. (Received data : 2005.05.25).
30. Samson RA, Hoekstra ES, Frisvad JC, Filtenborg O. (2002) Introduction to Food and Airborne Fungi, 6nd ed. Utrecht: CBS; 2002.
31. Gall GAE, Crandell PA. The rainbow trout. Aquaculture. 1992;100:1-10. [DOI]
32. Sarafi O, Faezi Ghasemi M, Chaiechi Nosrati A. Isolation and characterization of toxicogenic fungi strains from wheat and corn used in Kerman city. Journal of Microbial World. 2016;8(4):330-36.
33. Hedayati MT, Pasqualotto AC, Warn PA, Bowyer P. Aspergillus flavus: Human pathogen, allergen and mycotoxin producer. Microbiology. 2007;153:1677-92. [DOI] [PubMed]
34. Labuda L, Tanvinova D, Fungi recovered from Slovakian poultry feed mixtures and their toxinogeniety. Ann Agric Environ Med. 2006;13(2):193-200. [PubMed]
35. Dutta TK, Das P. Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxine B1 from feeds in India. Mycopathologia. 2000;151(1):29-33. [DOI]
36. Cutuli MT, Cuellar A, Camara JM, Mateos A, Suarez G. Different media and methodologies for the detection of aflatoxin production by Aspergillus flavus strains isolated from trout feed. Mycopathologia. 1991;113(2):121–5. [DOI] [PubMed]
37. Fraga ME, Curvello F, Gatti, MJ, Cavaglieri, LR, Dalcero, AM, Rocha- Rosa CA. Potential aflatoxin and ochratoxin A production by Aspergillus species in poultry feed processing. Vet Res Commun. 2007;31(3):343-53. [DOI] [PubMed]
38. Keller KM, Queiroz BD, Keller LAM, Ribeiro JMM, Cavaglieri LR, Gonzalez Pereyra ML, et al. 2007. The mycobiota and toxicity of equine feeds. Vet Res Commun. 2007;31(8):1037-45. [DOI] [PubMed]
39. Charoen Pornsook K, kavisarasai P. Mycotoxins in animal feedstuffs of Thailand. KMITL Sci Tech J. 2006;6(1):25-8.
40. Fadaeifard F, Koorangi H, Rahimi E, Raiisy M, Pirzadeh R. Determination of ochratoxin A in the rainbow trout (Oncorhynchus mykiss) feed in Chaharmahal Va Bakhtiary province by ELISA assay. Journal of Food Hygiene. 2013;2(8):71-9.

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