year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 149-157 | Back to browse issues page

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Asgarpoor D, Bahrami M, Daneshamooz S, Ghasemi M. Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City . Iran J Med Microbiol. 2018; 11 (6) :149-157
1- Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran ,
2- Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
3- Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (5216 Views)
Background and Aims: Staphylococcus aureus is a gram-positive, non-motile, non-spore-forming, aerobic, and facultative anaerobic bacterium, which is colonized in anterior part of the human nasal cavity. The carrierschr('39') individuals working in the food industry are the main source of spreading food-borne diseases. Staphylococcal enterotoxins (SEs) are the key virulence factors in the staphylococcal food poisoning (SFP). The aim of this study was to identify the S. aureus enterotoxin genes of sea, seb and sec among healthy carriers.
Materials and Methods: In the current descriptive cross-sectional study, 136 nasal swab samples were collected from the personnel working at butchers, dairy stores, and fast food restaurants through Ardabil city. The samples were cultured and then confirmed using the biochemical tests. The DNA of S. aureus isolates was extracted to detect sea, seb and sec genes as markers for SEA, SEB and SEC enterotoxins using the PCR method.
Results: Among the 136 nasal swab samples, 46 (33.8%) were positive for S. aureus, that were confirmed by the presence of femA gene. Out of 46 isolates, the sea, seb and sec genes were found in 11 (23.9%), 6 (13%) and 5 (10.8%) isolates, respectively.
Conclusions: According to the findings, a significant percentage of the food-chain personnel were nasal carriers of enterotoxigenic S. aureus. Therefore, for prevention and distribution of Staphylococcal infections, screening program and control of such carriers are recommended.
Full-Text [PDF 858 kb]   (1080 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
Received: 2017/11/19 | Accepted: 2018/01/15 | ePublished: 2018/03/19

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