year 12, Issue 1 (March - April 2018)                   Iran J Med Microbiol 2018, 12(1): 6-15 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kashefi Pasandideh N, Habibi poor R, Tahmasebi H, Arabestani M R. The Relationship Between Vitamin K2 Concentration and Expression of mecA and blaZ Genes in Clinical Isolates of Multi Drug Resistant of Methicilin Resistance Staphylococcus aureus . Iran J Med Microbiol 2018; 12 (1) :6-15
1- Department of Microbiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran
2- Department of Microbiology, Zahedan University of Medical Sciences, Zahedan, Iran
3- Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran ,
Abstract:   (7072 Views)
Background and Aims: As one of the fat-soluble vitamins, vitamin K, can in some cases have intrinsic inhibitory effects on the activity of methicillin-resistant Staphylococcus aureus. The aim of this study, was to determine the effect of vitamin K2 on the expression of mecA and blaZ genes in Multi Drug Resistant Methicilin Resistance S. aureus isolates.
Materials and Methods: 76 clinical isolates of methicillin-resistant S.aureus were isolated by phenotypic tests. For treatment of isolates, concentrations of 1, 10, 50, 100, 200, 300 and 500 μg / ml of vitamin K2 and different time intervals were used. The q-PCR method was used to quantitatively evaluate the genes. It was also used to analyze the results of REST 2008 V3 and SPSS 16 software.
Results: The expression of blaZ and mecA genes was reduced only at a concentration of 500 μg / ml of vitamin K2. Additionally, the 48 and 72 hours incubation times had the best effect on the effect of vitamins on the genes studied. In the isolates from clinical specimens, ulcers and urine reduced the expression of mecA and blaZ genes. Also, there was a significant relationship between incubation time and clinical specimen type on decreasing the expression of the desired genes (P≤0.01, P≤0.05).
Conclusions: In accordance to the results of this study, the use of vitamin K2 can play an important role in the control of methicillin-resistant Staphylococcus aureus strains.

Full-Text [PDF 985 kb]   (2202 Downloads)    
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2017/11/15 | Accepted: 2018/02/12 | ePublished: 2018/05/14

1. Leejae S, Hasap L, Voravuthikunchai SP. Inhibition of staphyloxanthin biosynthesis in Staphylococcus aureus by rhodomyrtone, a novel antibiotic candidate. J med microbiol. 2013;62(3):421-8. [DOI] [PubMed]
2. Matheson EM, Mainous AG, Hueston WJ, Diaz VA, Everett CJ. Vitamin D and methicillin-resistant Staphylococcus aureus nasal carriage. Scand J Infect Dis. 2010;42(7):455-60. [DOI] [PubMed]
3. Khameneh B, Fazly Bazzaz BS, Amani A, Rostami J, Vahdati-Mashhadian N. Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains. Microb Pathog. 2016;93(11):83-7. PMid:26602814
4. Yuan G, Zhu X, Li P, Zhang Q, Cao J. New activity for old drug: In vitro activities of vitamin K3 and menadione sodium bisulfite against methicillin-resistant Staphylococcus aureus. Afr J Pharm Pharmacol. 2014;8(17):451-54. [DOI]
5. Khazaei S, Pourtahmaseby P, Kanani M, Madani SH, Malekianzadeh E. Staphylococcus Aureus Resistance to Vancomycin: A Six Years Survey,(2006-2012). Med J Tabriz Univ Med Sci. 2013;35(3):40-5.
6. Pourmand MR, Yousefi M, Salami SA, Amini M. Evaluation of expression of NorA efflux pump in ciprofloxacin resistant Staphylococcus aureus against hexahydroquinoline derivative by real-time PCR. Acta medica Iranica. 2014;52(6):424-9. [DOI]
7. Imani Fooladi AA, Ashrafi E, Tazandareh SG, Koosha RZ, Rad HS, Amin M, et al. The distribution of pathogenic and toxigenic genes among MRSA and MSSA clinical isolates. Microb pathog. 2015;81(9):60-6. [DOI] [PubMed]
8. Srinivasan A, Bankowski MJ, Seifried SE, Jinno S, Perkins R, Singh S, et al. A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton–Valentine leukocidin and tst virulence genes. Diagn Microbiol Infect Dis. 2011;70(4):541-3. [DOI] [PubMed]
9. Hu DL, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, et al. Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates. J med microbiol. 2008;57(9):1106-12. [DOI] [PubMed]
10. Tintino SR, Morais-Tintino CD, Campina FF, Pereira RL, Costa MS, Braga FB, et al. Action of cholecalciferol and alpha-tocopherol on Staphylococcus aureus efflux pumps. EXCLI j. 2016;15:31(5)-22.
11. Witt M, Kvist N, Jorgensen MH, Hulscher JB, Verkade HJ. Prophylactic Dosing of Vitamin K to Prevent Bleeding. Pediatrics. 2016;137(5):1-10. [DOI] [PubMed]
12. Andrade JC, Braga FB, Guedes GMM, Tintino SR, Freitas MA, Quintans LJ, et al. Menadione (vitamin K) enhances the antibiotic activity of drugs by cell membrane permeabilization mechanism. Saudi J Biol Sci. 2017;24(1):59-64. [DOI] [PubMed]
13. Pierpaoli E, Cirioni O, Barucca A, Orlando F, Silvestri C, Giacometti A, et al. Vitamin E supplementation in old mice induces antimicrobial activity and improves the efficacy of daptomycin in an animal model of wounds infected with methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother. 2011;66(9):2184-5. [DOI] [PubMed]
14. Wang JW, Hogan PG, Hunstad DA, Fritz SA. Vitamin D sufficiency and Staphylococcus aureus infection in children. Pediatr Infect Dis J. 2015;34(5):544-5. [DOI] [PubMed]
15. Lee KM, Go J, Yoon MY, Park Y, Kim SC, Yong DE, et al. Vitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa. Infect Immun. 2012;80(5):1639-49. [DOI] [PubMed]
16. Bokaeian M, Tahmasebi H. Molecular Identification of responsible resistance to aminoglycosides genes in clinical samples of Staphylococcus aureus. J Babol Univ Med Sci. 2017;19(3):38-46.
17. CLSI. M100-S25 performance standards for antimicrobial susceptibility testing. Twenty-fifth informational supplement; 2015.
18. Ares M. Bacterial RNA isolation. Cold Spring Harb Protoc. 2012;20(9):1024-7. [DOI]
19. Pfaffl WM. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res. 2001;29(11):45. [DOI]
20. Chan W-S, Chan TM, Lai TW, Chan JFW, Lai RWM, Lai CKC, et al. Complementary use of MALDI-TOF MS and real-time PCR–melt curve analysis for rapid identification of methicillin-resistant staphylococci and VRE. J Antimicrob Chemother. 2015;70(2):441-7. [DOI] [PubMed]
21. Robles BF, Nóbrega DB, Guimarães FF, Wanderley GG, Langoni H. Beta-lactamase detection in Staphylococcus aureus and coagulase-negative Staphylococcus isolated from bovine mastitis. Pesqui Vet Bras. 2014;34(9):325-8. [DOI]
22. Larsen AR, Petersen A, Holmes M, Kearns A, Hill R, Edwards G, et al. Utility of a newly developed Mueller-Hinton E agar for the detection of MRSA carrying the novel mecA homologue mecC. J Antimicrob Chemother. 2015;70(4):1256-7. [PubMed]
23. Becker K, Denis O, Roisin S, Mellmann A, Idelevich EA, Knaack D, et al. Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay. J Clin Microbiol. 2016;54(1):180-4. [DOI] [PubMed]
24. Tahmasebi H, Zeiyni B, Dehbashi S, Motamedi H, Vafaeifar M, Keramat F, et al. The Study of blaZ and mecA Gene Expression in Methicillin-Resistant Staphylococcus Aureus Strains and the Relationship between the Gene Expression Patterns. JIMS. 2017;35(433):1062-7.
25. George L, Bavya MC, Rohan KV, Srivastava R. A therapeutic polyelectrolyte–vitamin C nanoparticulate system in polyvinyl alcohol–alginate hydrogel: An approach to treat skin and soft tissue infections caused by Staphylococcus aureus. Colloids Surf B Biointerfaces. 2017;160(4):315-24. [DOI] [PubMed]
26. Yue Y, Hymøller L, Jensen SK, Lauridsen C, Purup S. Effects of vitamin D and its metabolites on cell viability and Staphylococcus aureus invasion into bovine mammary epithelial cells. Vet microbiol. 2017;203(5):245-51. [DOI] [PubMed]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc