year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 210-216 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Beyzaei H, Ghasemi B, Mirzaei M, Sanjarani G. Comparison of Inhibitory Effects of Nisin, Glycine, Poly-L-lysine, Magnesium Oxide Nanoparticles and Peganum harmala Extract on Standard and Multidrug-Resistant Pseudomonas aeruginosa. Iran J Med Microbiol. 2018; 11 (6) :210-216
URL: http://ijmm.ir/article-1-786-en.html
1- Department of Chemistry, Faculty of Science, University of Zabol, Zabol, Iran , hbeyzaei@yahoo.com
2- Torbat-e Jam Faculty of Medical Sciences, Torbat-e Jam, Iran
3- Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4- Young Researchers and Elite Club, Zahedan Branch, Islamic Azad University, Zahedan, Iran
Abstract:   (4675 Views)
Background and Aims: Pseudomonas aeruginosa is one of the most important common causes of nosocomial infections that its standard and antibiotic-resistant strains can infect critical body organs such as the lungs and the urinary tract. In this study, inhibitory effects of nisin, glycine, poly-L-lysine, magnesium oxide nanoparticles and hydroalcoholic extract of Peganum harmala were assessed against standard and multidrug-resistant strains of P. aeruginosa.
Materials and Methods: MgO nanoparticles with sizes of around 30-50 nm were synthesized via wet chemical method. Their structure were characterized using X-ray diffraction (XRD) analysis and scanning electron microscope (SEM). P. harmala seed extract was prepared by soaking in water-ethanol 1:1. Solutions of glycine, poly-L-lysine and nisin were prepared, and sterilized using 0.22 μm filter. Inhibitory effects of all compounds as inhibition zone diameter (IZD), the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values were determined according to CLSI guidelines using disk diffusion and broth microdilution methods.
Results and Conclusions: No significant inhibitory effects against P. aeruginosa were observed at the highest used concentrations of glycine, poly-L-lysine, MgO nanoparticles and plant extract. Inhibitory effects against both standard and resistant strains were only recorded for nisin with inhibition zone diameter of 10.36, 13.08 mm, MIC of 64, 128 μg/mL and MBC of 256, 512 μg/mL. Nisin as a natural food preservative can be used alone or in combination with current antibiotics to treat diseases caused by P. aeruginosa.
Full-Text [PDF 750 kb]   (1094 Downloads)    
Type of Study: Brief report | Subject: Antimicrobial Substances
Received: 2017/11/13 | Accepted: 2018/02/12 | ePublished: 2018/03/19

References
1. Xie J, Yang L, Peters BM, Chen L, Chen D, Li B et al. A 16-year retrospective surveillance report on the pathogenic features and antimicrobial susceptibility of Pseudomonas aeruginosa isolates from FAHJU in Guangzhou representative of Southern China. Microb Pathog. 2017 Sep;110:37–41. [DOI] [PubMed]
2. Samadi M, Shekarforoush SS, Ghaisari HR. Antimicrobial effects of magnesium oxide nanoparticles and ε-poly-L-lysine against Escherichia coli O157:H7 and Listeria monocytogenes. Int J Med Microbiol. 2016;10(2):33–41.
3. Minami M, Ando T, Hashikawa SN, Torii K, Hasegawa T, Israel DA et al. Effect of glycine on Helicobacter pylori in vitro. Antimicrob Agents Chemother. 2004 Oct;48(10):3782–8. [DOI] [PubMed]
4. de Oliveira Junior AA, de Araújo Couto HG, Barbosa AA, Carnelossi MA, de Moura TR. Stability, antimicrobial activity, and effect of nisin on the physico-chemical properties of fruit juices. Int J Food Microbiol. 2015 Oct;211:38–43. [DOI] [PubMed]
5. Stevens KA, Klapes NA, Sheldon BW, Klaenhammer TR. Antimicrobial action of nisin against Salmonella typhimurium lipopolysaccharide mutants. Appl Environ Microbiol. 1992 May;58(5):1786–8. [PubMed]
6. Akerey B, Le-Lay C, Fliss I, Subirade M, Rouabhia M. In vitro efficacy of nisin Z against Candida albicans adhesion and transition following contact with normal human gingival cells. J Appl Microbiol. 2009 Oct;107(4):1298–307. [DOI] [PubMed]
7. Bindhu MR, Umadevi MM, Micheal K, Arasu MV, Al-Dhabi N. Structural, morphological and optical properties of MgO nanoparticles for antibacterial applications. Mater Lett. 2016;166:19–22. [DOI]
8. Mahmoudian M, Jalipour H, Dardashti PS. Toxicity of Peganum harmala: review and a case report. Iran J Pharmacol Ther. 2002;1(1):1–4.
9. Zeinali T, Mohsenzadeh M, Rezaeian-Doloei R, Nabipour R. In vitro assessment of antimicrobial effect of methanolic extract of Peganum harmala against some important foodborne bacterial pathogens. J Food Saf Hyg. 2016;5(20):27–36.
10. Beyzaei H, Aryan R, Molashahi H, Zahedi MM, Samzadeh Kermani A, Ghasemi B et al. MgO nanoparticle catalyzed, solvent free Hantzsch synthesis and antibacterial evaluation of new substituted thiazoles. J Iran Chem Soc. 2017;14(5):1023–31. [DOI]
11. Safari R, Yaghoubzadeh Z. The combined effect of nisin, sodium acetate to increase the shelf life of trout (Onchorhynchus mykiss) in form completely empty stomach. Iran Sci Fish J. 2016;24(4):155–69.
12. Scoparo CT, Borato DG, Souza LM, Dartora N, Luisa M, Maria-Ferreira D et al. Gastroprotective bio-guiding fractionation of hydro-alcoholic extracts from green- and black-teas (Camellia sinensis). Food Res Int. 2017;64:577–86. [DOI]
13. Beyzaei H, Ghasemi B. Study of antibacterial effect of thirty new heterocyclic derivatives against Bacillus subtilis and salmonella enterica. Iran J Infect Dis Trop Med. 2016;20(71):1–8.
14. Landers TF, Cohen B, Wittum TE, Larson EL. A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Rep. 2012 Jan-Feb;127(1):4–22. [DOI] [PubMed]
15. Krishnamoorthy K, Manivannan G, Kim SJ, Jeyasubramanian K, Premanathan M. Antibacterial activity of MgO nanoparticles based on lipid peroxidation by oxygen vacancy. J Nanopart Res. 2012;14(9):1063. [DOI]
16. Wei L, Wu R, Wang C, Wu Z. Effects of ε-polylysine on Pseudomonas aeruginosa and Aspergillus fumigatus biofilm in vitro. Med Sci Monit. 2017 Sep;23:4225–9. [DOI] [PubMed]
17. Hyldgaard M, Mygind T, Vad BS, Stenvang M, Otzen DE, Meyer RL. The antimicrobial mechanism of action of epsilon-poly-l-lysine. Appl Environ Microbiol. 2014 Dec;80(24):7758–70. [DOI] [PubMed]
18. Gerberick GF, Castric PA. In vitro susceptibility of Pseudomonas aeruginosa to carbenicillin, glycine, and ethylenediaminetetraacetic acid combinations. Antimicrob Agents Chemother. 1980 Apr;17(4):732–5. [DOI] [PubMed]
19. Mazandarani M, Ghaemi EA, Ghaffari F. Antibacterial survey of different extracts of Peganum harmala L. different parts in Northeast of Golestan province (Inche Borun). J Plant Sci Res. 2009;15(3):27–38.
20. Liu H, Pei H, Han Z, Feng G, Li D. The antimicrobial effects and synergistic antibacterial mechanism of the combination of ε-polylysine and nisin against Bacillus subtilis. Food Control. 2015;47:444–50. [DOI]
21. Dielbandhoesing SK, Zhang H, Caro LH, van der Vaart JM, Klis FM, Verrips CT et al. Specific cell wall proteins confer resistance to nisin upon yeast cells. Appl Environ Microbiol. 1998 Oct;64(10):4047–52. [PubMed]
22. Stevens KA, Sheldon BW, Klapes NA, Klaenhammer TR. Nisin treatment for inactivation of Salmonella species and other gram-negative bacteria. Appl Environ Microbiol. 1991 Dec;57(12):3613–5. [PubMed]
23. Zhou L, van Heel AJ, Montalban-Lopez M, Kuipers OP. Potentiating the activity of nisin against Escherichia coli. Front Cell Dev Biol. 2016 Feb;4:7. [DOI] [PubMed]
24. Khan A, Vu KD, Riedl B, Lacroix M. Optimization of the antimicrobial activity of nisin, Na-EDTA and pH against gram-negative and gram-positive bacteria. Lebensm Wiss Technol. 2015;61(1):124–9. [DOI]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc