year 13, Issue 1 (March - April 2019)                   Iran J Med Microbiol 2019, 13(1): 14-21 | Back to browse issues page

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Majdi M, Khazaei Koohpar Z, Nasrollahi Omran A. Investigation of Mutations of ERG11 Gene in Fluconazole Resistant Strains of Candida Albicans Isolated From Patients With Volvovaginitis in West of Mazandaran. Iran J Med Microbiol 2019; 13 (1) :14-21
1- Department of Cell and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
2- Department of Cell and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran ,
3- Department of Medical Mycology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Abstract:   (3330 Views)
Background and Aims: Candida albicans as an opportunistic fungal pathogen in human is the cause of volvovaginitis candidiasis. Azole resistance is cinsiderable as worldwide problem in treatment of candidiasis. Azole resistance can occur through different mechanisms such as mutation in ERG11 gene. The aim of our study was evaluation of ERG11 gene mutations in fluconazole resistant islotes of C. albicans obtained from patients with volvovaginitis in west of Mazandaran.
Materials and Methods: In this study, clinical specimens were obtained from vaginal mucosa of 120 individual. C. albicans isolates were identified by standard methods such as germ tubes and culture in chrome agar media. Susceptibility test to fluconazole in the isolates was evaluated by Disc diffusion and MIC methods. After DNA extraction, ERG11 gene mutations in clinical isolates were determined by PCR- sequencing method.
Results: From 45 isolates of C. albicans, 40 isolates were resistant to fluconazole. The MIC of fluconazole in isolates was determined between 2 to 64μg/ml. Also, sequencing analysis showed that 7 fluconazole resistant isolates had three missense mutations (D116E, K128T and A114S) in ERG11 gene.
 Conclusion: It apears that high frequency of floconazol resistance is the results of different reasons such as mutations in ERG11 gene in C. albicans isolates.

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2017/10/23 | Accepted: 2019/05/16 | ePublished: 2019/07/22

1. Gavanji S, Larki B. Comparative Effect of Propolis of Honey Bee and Some Herbal Extracts on Candida Albicans. Chinese Journal of Integrative Medicine. 2017; 23(3):201-7. [DOI:10.1007/s11655-015-2074-9] [PMID]
2. Kabir MA, Hussain MA, Ahmad Z. Candida Albicans: A Model Organism for Studying Fungal Pathogens. ISRN Microbiology. 2012; 2012:538694. [DOI:10.5402/2012/538694] [PMID] [PMCID]
3. Pam VK, Akpan JU, Oduyebo OO, Nwaokorie FO, Fowora MA, Oladele RO, et al. Fluconazole Susceptibility and Erg11 Gene Expression in Vaginal Candida Species Isolated From Lagos Nigeria. International Journal of Molecular Epidemiology and Genetics. 2012;3(1):84-90.
4. Reiss E, Tanaka K, Bruker G, Chazalet V, Coleman D, Debeaupuis JP, et al. Molecular Diagnosis and Epidemiology of Fungal Infections. Medical Mycology. 1998; 36(Suppl 1):249-57.
5. Sanglard D, Ischer F, Parkinson T, Falconer D, Bille J. Candida Albicans Mutations in the Ergosterol Biosynthetic Pathway and Resistance to Several Antifungal Agents. Antimicrobial Agents and Chemotherapy. 2003; 47(8):2404-12. [DOI:10.1128/AAC.47.8.2404-2412.2003] [PMID] [PMCID]
6. Cernicka J, Subik J. Resistance Mechanisms in Fluconazole-Resistant Candida Albicans Isolates From Vaginal Candidiasis. International Journal of Antimicrobial Agents. 2006; 27(5):403-8. [DOI:10.1016/j.ijantimicag.2005.12.005] [PMID]
7. Flowers SA, Colon B, Whaley SG, Schuler MA, Rogers PD. Contribution of Clinically Derived Mutations in Erg11 to Azole Resistance in Candida Albicans. Antimicrobial Agents and Chemotherapy. 2015; 59(1):450-60. [DOI:10.1128/AAC.03470-14] [PMID] [PMCID]
8. Balabandi S, Khazaei -Koohpar Z, Ranji N. Correlation Between Erg11 Gene Mutations and Fluconazole Resistance in Candida Albicans Strains Isolates Isolated in From Rasht in 2015-2016 Years. Arak Medical University Journal. 2017; 20(7):13-22.
9. White TC, Holleman S, Dy F, Mirels LF, Stevens DA. Resistance Mechanisms in Clinical Isolates of Candida Albicans. Antimicrobial Agents and Chemotherapy. 2002; 46(6):1704-13. [DOI:10.1128/AAC.46.6.1704-1713.2002] [PMID] [PMCID]
10. Wang B, Huang LH, Zhao JX, Wei M, Fang H, Wang DY, et al. ERG11 Mutations Associated With Azole Resistance in Candida Albicans Isolates From Vulvovaginal Candidosis Patients. Asian Pacific Journal of Tropical Biomedicine. 2015; 5(11):909-14. [DOI:10.1016/j.apjtb.2015.08.002]
11. Xiang MJ, Liu JY, Ni PH, Wang S, Shi C, Wei B, et al. Erg11 Mutations Associated With Azole Resistance in Clinical Isolates of Candida Albicans. FEMS Yeast Research. 2013; 13(4):386-93. [DOI:10.1111/1567-1364.12042] [PMID]
12. Manastir L, Ergon MC, Yucesoy M. Investigation of Mutations in Erg11 Gene of Fluconazole Resistant Candida Albicans Isolates From Turkish Hospitals. Mycoses. 2011; 54(2):99-104. [DOI:10.1111/j.1439-0507.2009.01766.x] [PMID]
13. Debnath S, Addya S. Structural Basis for Heterogeneous Phenotype of ERG11 Dependent Azole Resistance in C.Albicans Clinical Isolates. SpringerPlus. 2014; 3:660. [DOI:10.1186/2193-1801-3-660] [PMID] [PMCID]
14. Xu Y, Chen L, Li C. Susceptibility of Clinical Isolates of Candida Species to Fluconazole and Detection of Candida Albicans ERG11 Mutations. The Journal of Antimicrobial Chemotherapy. 2008; 61(4):798-804. [DOI:10.1093/jac/dkn015] [PMID]
15. Ungureanu A, Gaman AE, Turculeanu A, Mitroi M, Drocas AI, Dobritoiu M, et al. Incidence and Antifungal Susceptibility of Candida Albicans Infections. Current Health Sciences Journal. 2016; 42(2):164-8.
16. Farahbakhsh E, Yadegari MH, Rajabi Bazl M, Taghizadeh Armaki M, Katiraee F. Identification of Fluconazole Resistance Gene Erg11 in Clinical Candida Albicans Samples Isolated From Hiv-Infected Patients by Reverse Polymerase Chain Reaction (RT-PCR). Daneshvar. 2012; 19(96):19-28.
17. Nasrollahi Omran A, Nazemi A, Kihanian S, Aryana N. Lipase Gene Expression of Resistant and Sensitive Candida Albicans to Fluconazole Isolated From Patients Suffering From Oral Candidiasis and Vaginal Candidiasis. Medical Laboratory Journal. 2015; 8(5):90-96.
18. Mohammadi-Ghalehbin B, Javanpour Heravi H, Arzanlou M, Sarvi M. Prevalence and Antibiotic Resistance Pattern of Candida spp; Isolated From Pregnant Women Referred to Health Centers in Ardabil, Iran. Journal of Ardabil University of Medical Sciences. 2017; 16(4):409-421.
19. Rosana Y, Yasmon A, Lestari DC. Overexpression and Mutation as a Genetic Mechanism of Fluconazole Resistance in Candida Albicans Isolated From Human Immunodeficiency Virus Patients in Indonesia. Journal of Medical Microbiology. 2015; 64(9):1046-1052. [DOI:10.1099/jmm.0.000123] [PMID]
20. Perea S, Lopez-Ribot JL, Kirkpatrick WR, McAtee RK, Santillan RA, Martinez M, et al. Prevalence of Molecular Mechanisms of Resistance to Azole Antifungal Agents in Candida Albicans Strains Displaying High-Level Fluconazole Resistance Isolated From Human Immunodeficiency Virus-Infected Patients. Antimicrobial Agents and Chemotherapy. 2001; 45(10):2676-2684. [DOI:10.1128/AAC.45.10.2676-2684.2001] [PMID] [PMCID]

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