year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 192-202 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rasooli H, Ghorbanalinezhad E. Isolation and Identification of Methicillin Resistant Staphylococcus aureus Based on hla , lukED, sei, and hlg Virulence Genes in Patients with Diabetic Foot Infection in Mazandaran Province. Iran J Med Microbiol. 2018; 11 (6) :192-202
1- Department of Microbiology, School of Biology Sciences, Islamic Azad University of Tonekabon branch, Tonekabon, Iran
2- Department of Microbiology, School of Biology Sciences, Islamic Azad University of Tonekabon Branch, Tonekabon, Iran ,
Abstract:   (5310 Views)
Background and Aims: Methicillin-resistant Staphylococcus aureus is the most common bacterial agent of diabetic foot Infection (DFI).The Virulence factors of this bacterium increases the severity of the degree of wound infection, and if not treated promptly, results in lower limb amputation and death of the affected patients. The aim of this study was to isolate methicillin resistant Staphylococcus aureus carries the mecA gene and to detect 4 Virulence genes hla, lukED, sei and hlg from patients with diabetic foot infection in order to evaluate their role in severity of infection.
Materials and Methods: 30 cases of diabetic foot infections in Mazandaran province were collected from pus drainage and Staphylococcus aureus was purified according to culture characteristics and biochemical tests. The molecular evaluation of the isolates after DNA extraction was performed with five pairs of specific primers of intended genes, by PCR method.
 Results: Of the 30 patients, 14 isolates (46.6%) of S. aureus were isolated and identified. Each 14 samples contained mecA gene and the frequency of hla, lukED, sei and hlg genes in isolates was 100%, 100%, 71.4% and 64.2%, respectively.
 Conclusions: Identification of these 4 genes with the mecA gene in S. aureus isolated from diabetic foot Infection can be an effective and reliable genetic marker for diagnosis of foot infection in diabetic patients. To prevent amputation, diagnosis of infection by PCR method and appropriate timely antibiotic therapy are required for Diabetic patients.

Full-Text [PDF 923 kb]   (2286 Downloads)    
Type of Study: Original | Subject: Molecular Microbiology
Received: 2017/09/18 | Accepted: 2018/01/30 | ePublished: 2018/03/19

1. Isselbacher KJ, Braunwald E, Wilson JD, Martin JB, Fauci A.S. and L.M. Kasper, Harrison's Principles of Internal Medicine, , 17th ed.New York: Mc Graw-Hill; 2008. [DOI] [PubMed]
2. Shaw J.E, Sicree P.Z. and P.Z Zimmet, Global estimates of the prevalence of diabetes for 2010 and 2030, Diabetes Res Clin Pract, 2010, 87(1):4-14.
3. Boulton AJ, Vileikyte L, Ragnarson-Tennvall G, J. Apelqvist, The global burden of diabetic foot disease, 2005 Nov 12; 366(9498):1719-24.
4. Lipsky B, Peters E, Senneville E, Berendt A, Embil J, Lavery L, et al. Expert opinion on the management of infections in the diabetic foot. Diabetes Metab Res Rev. 2012;28(S1):163-78. [DOI] [PubMed]
5. Alami Harandi B, Alami Harandi A, Siavashi B. Diabetic foot ulcer management (Review of Literature). Iranian J of Surg. 2007;16(4):1-7.
6. Plata K, Rosato AE, Wegrzyn G. Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity. Acta Biochim Pol. 2009;56(4):597. [PubMed]
7. Katayama Y, Zhang H-Z, Hong D, Chambers HF. Jumping the barrier to β-lactam resistance in Staphylococcus aureus. J Bacteriol. 2003;185(18):5465-72. [DOI] [PubMed]
8. Zhang K, McClure J-A, Elsayed S, Conly JM. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2009;53(2):531-40. [DOI] [PubMed]
9. Deurenberg RH, Stobberingh EE. The molecular evolution of hospital-and community-associated methicillin-resistant Staphylococcus aureus. Curr Mol Med. 2009;9(2):100-15. [DOI]
10. Peck KR, Baek JY, Song J-H, Ko KS. Comparison of genotypes and enterotoxin genes between Staphylococcus aureus isolates from blood and nasal colonizers in a Korean hospital. J Korean Med Sci. 2009;24(4):585-91. [DOI] [PubMed]
11. Schubert S, Schwertz H, Weyrich AS, Franks ZG, Lindemann S, Otto M, et al. Staphylococcus aureus α-toxin triggers the synthesis of B-cell lymphoma 3 by human platelets. Toxins. 2011;3(2):120-33. [DOI] [PubMed]
12. Dinges MM, Orwin PM, Schlievert PM. Exotoxins of Staphylococcus aureus. Clin Microbiol Rev. 2000;13(1):16-34. [DOI] [PubMed]
13. Fueyo J, Mendoza M, Rodicio M, Muniz J, Alvarez M, Martín M. Cytotoxin and pyrogenic toxin superantigen gene profiles of Staphylococcus aureus associated with subclinical mastitis in dairy cows and relationships with macrorestriction genomic profiles. J Clin Microbiol. 2005;43(3):1278-84. [DOI] [PubMed]
14. Prevost G, Cribier B, Couppie P, Petiau P, Supersac G, Finck-Barbancon V, et al. Panton-Valentine leucocidin and gamma-hemolysin from Staphylococcus aureus ATCC 49775 are encoded by distinct genetic loci and have different biological activities. Infect Immun. 1995;63(10):4121-9. [PubMed]
15. Munson SH, Tremaine MT, Betley MJ, Welch RA. Identification and characterization of staphylococcal enterotoxin types G and I fromStaphylococcus aureus. Infect Immun. 1998;66(7):3337-48. [PubMed]
16. Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter M-O, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin—producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999;29(5):1128-32. [DOI] [PubMed]
17. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, et al. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun. 2002;70(2):631-41. [DOI] [PubMed]
18. Ryffel C, Tesch W, Birch-Machin I, Reynolds PE, Barberis-Maino L, Kayser FH, et al. Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Gene. 1990;94(1):137-8. [DOI]
19. Shah BR, Hux JE. Quantifying the risk of infectious diseases for people with diabetes. Diabetes Care. 2003; 26(2):510-3. [DOI]
20. Styers D, Sheehan DJ, Hogan P, Sahm DF. Laboratory-based surveillance of current antimicrobial resistance patterns and trends among Staphylococcus aureus: 2005 status in the United States. Ann Clin Microbiol Antimicrob. 2006;5(1):2. [DOI] [PubMed]
21. Sotto A, Lina G, Richard J-L, Combescure C, Bourg G, Vidal L, et al. Virulence potential of Staphylococcus aureus strains isolated from diabetic foot ulcers: a new paradigm. Diabetes Care. 2008;31(12):2318-24. [DOI] [PubMed]
22. El-baz R, Dina Eid Rizk D, Barwa R, Hassan R. Virulence factors profile of Staphylococcus aureus isolated from different clinical sources in Egypt. J Microbiol. 2016;43:126-44
23. Abdel-hamed A-HA, Abdel-Rhman SH, El-Sokkary MA. Studies on leukocidins toxins and antimicrobial resistance in Staphylococcus aureus isolated from various clinical sources. Afr J Microbiol Res. 2016;10(17):591-9. [DOI]
24. Alfatemi SMH, Motamedifar M, Hadi N, Saraie HSE. Analysis of virulence genes among methicillin resistant Staphylococcus aureus (MRSA) strains. Jundishapur J Microbiol. 2014;7(6).
25. Sumeet S, Irani U, Flavia R. Prevalence of methicillin resistance and virulence determinants of Staphylococcus aureus in diabetic foot ulcers. Int J Basic Clin Pharmacol. 2014;3(6):978-82.
26. Sauer P, Síla J, Štosová T, Večeřová R, Hejnar P, Vágnerová I, et al. Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic. J Med Microbiol. 2008;57(4):403-10. [DOI] [PubMed]
27. Dibah S, Arzanlou M, Jannati E, Shapouri R. Prevalence and antimicrobial resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens in Ardabil, Iran. Iran J Microbiol. 2014;6(3):163-8. [PubMed]
28. Ahmadi A, Tajbakhsh E, Momtaz H. Detection of the antibiotic resistance pattern in Staphylococcus aureus isolated from clinical samples obtained from patients hospitalised in Imam Reza hospital, Kermanshah. J Microbial World. 2014;6(4):299-311.
29. Zeinali E, Moniri R, Safari M, Mousavi GA. Molecular characterization and SCCmec typingin in meticillin-resistant Staphylococcus aureus isolated from clinical samples. Feyz J Kashan Uni Med Sci. 2011;14(4):439-46.
30. Al-Ruaily, Khalil OM. Detection of mecA gene in methicillin resistant Staphylococcus aureus (MRSA) at Prince A / Rhman, Sidery Hospital, Al-Jouf, Saudi Arabia . J Med Genet Genomic.2011;3(3):41-5.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc