year 11, Issue 5 (November - December 2017)                   Iran J Med Microbiol 2017, 11(5): 136-148 | Back to browse issues page

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Hoseynzadeh A, Khaleghi M, Sasan H. Investigating the Antimicrobial Effects of Silver Nanoparticles Synthesized by Bacteria Isolated From Agricultural Soils of Kerman, Iran . Iran J Med Microbiol. 2017; 11 (5) :136-148
1- Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
2- Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran ,
Abstract:   (6353 Views)

Background and Aims: The use of microorganisms in the synthesis of metal nanoparticles is known as an eco-friendly method. Moreover, because of the ability of microorganisms to synthesize nanoparticles of various sizes, shapes and morphologies, this method has gained extreme attentions in recent years. The aim of this study was, therefore, to investigate the antimicrobial effects of silver nanoparticles synthesized by bacteria isolated from agricultural soils of Kerman, Iran.
Materials and Methods: This study was carried out in 2014. Silver nanoparticles were characterized by SEM, EDS and XRD analyzes. The antimicrobial effects of silver nanoparticles were also assessed against some pathogenic bacteria.
Results: Of the 40 silver nanoparticle producing bacteria, the strains that were able to produce silver nanoparticles with high antimicrobial activity yielded under different environmental conditions, were selected. The results of scanning electron microscopy (SEM) confirmed the presence of silver nanoparticles with a spherical shape. EDS analysis showed that silver content of the particles was about 60 wt %. Sequence alignment and phylogenetic tree results showed that M9 and B7 strains are closely related to Bacillus cereus and Pseudomonas argentinensis, respectively, with 99% homology.
Conclusions: The results showed that the M9 and B7 strains can synthesize silver nanoparticles with high antimicrobial effects under different environmental conditions. 

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Type of Study: Original | Subject: Nanotechnology In Medicine
Received: 2017/07/1 | Accepted: 2017/09/17 | ePublished: 2017/11/20

1. Krishnaraj C, Jagan E, Rajasekar S, Selvakumar P, Kalaichelvan P, Mohan N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water born pathogens. Colloids Surf B Biointerfaces 2010;76(1): 50-6. [DOI] [PubMed]
2. Prasad K, Jha AK, Kulkarni A. Lactobacillus assisted synthesis of titanium nanoparticles. Nanoscale Res Lett 2007;2(5):248-50. [DOI] [PubMed]
3. Das VL, Thomas R, Varghese RT, Soniya E, Mathew J, Radhakrishnan E. Extracellular synthesis of silver nanoparticles by the Bacillus strain CS 11 isolated from industrialized area. 3 Biotech 2014;4(2):121-6. [DOI] [PubMed]
4. Pourali P, Yahyaei B. Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. J Trace Elem Med Biol 2016;34:22-31. [DOI] [PubMed]
5. Kannan N, Selvaraj S, Murty RV. Microbial production of silver nanoparticles. Dig J nanomater biostruct 2010;5(1):135-40.
6. Nithya R, Ragunathan R. Synthesis of silver nanoparticle using Pleurotus sajor caju and its antimicrobial study. Dig J nanomater biostruct 2009;4(4):623-9.
7. Tran QH, Le AT. Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Adv Nat Sci: Nanosci Nanotechnol 2013;4(3):033001. [DOI]
8. Kushwaha A, Singh VK, Bhartariya J, Singh P, Yasmeen K. Isolation and identification of E. coli bacteria for the synthesis of silver nanoparticles: characterization of the particles and study of antibacterial activity. Eur J Exp Biol 2015;5(1):65-70.
9. Singh P, Singh H, Kim YJ, Mathiyalagan R, Wang C, Yang DC. Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications. Enzyme microb technol 2016;86:75-83. [DOI] [PubMed]
10. Lengke MF, Fleet ME, Southam G. Biosynthesis of silver nanoparticles by filamentous cyanobacteria from a silver (I) nitrate complex. Langmuir 2007;23(5):2694-9. [DOI] [PubMed]
11. Prabhu S, Poulose EK. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int nano lett 2012;2(1):32. [DOI]
12. Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr sci 2003;85(2):162-70.
13. Remya RR, Radhika Rajasree SR, Aranganathan L, Suman TY. An investigation on cytotoxic effect of bioactive AgNPs synthesized using Cassia fistula flower extract on breast cancer cell MCF-7. Biotechnol Rep 2015; 8: 110-5. [DOI] [PubMed]
14. Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi A-A. Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Process Biochem 2007;42(5):919-23. [DOI]
15. Jain N, Bhargava A, Majumdar S, Tarafdar J, Panwar J. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective. Nanoscale 2011;3(2):635-41. [DOI] [PubMed]
16. Kalishwaralal K, Deepak V, Pandian SRK, Kottaisamy M, BarathManiKanth S, Kartikeyan B, et al. Biosynthesis of silver and gold nanoparticles using Brevibacterium casei. Colloids Surf B Biointerfaces 2010;77(2):257-62. [DOI] [PubMed]
17. Kalimuthu K, Babu RS, Venkataraman D, Bilal M, Gurunathan S. Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids Surf B Biointerfaces 2008;65(1):150-3. [DOI] [PubMed]
18. Saravanan M, Vemu AK, Barik SK. Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens. Colloids Surf B Biointerfaces 2011;88(1):325-31. [DOI] [PubMed]
19. Zaki S, El Kady M, Abd-El-Haleem D. Biosynthesis and structural characterization of silver nanoparticles from bacterial isolates. Mater Res Bull 2011;46(10):1571-6. [DOI]
20. Levard Cm, Mitra S, Yang T, Jew AD, Badireddy AR, Lowry GV, et al. Effect of chloride on the dissolution rate of silver nanoparticles and toxicity to E. coli. Environ Sci Technol 2013;47(11):5738-45. [DOI] [PubMed]
21. Al Qadi B, Toshiharu S, editors. A study of optical anisotropy for nearly spherical gold nanoparticles. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications. Proc SPIE 2009;7190: 719016-20. [DOI]
22. Cheng HR, Jiang N. Extremely rapid extraction of DNA from bacteria and yeasts. Biotechnol lett 2006. 28(1): 55-9. [DOI] [PubMed]
23. El-Batal A, Amin M, Shehata MM, Hallol MM. Synthesis of silver nanoparticles by Bacillus stearothermophilus using gamma radiation and their antimicrobial activity. World Appl Sci J 2013;22(1):01-16.
24. Iravani S, Korbekandi H, Mirmohammadi S, Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods. Res pharm sci 2014;9(6):385.
25. Thomas R, Janardhanan A, Varghese RT, Soniya E, Mathew J, Radhakrishnan E. Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp. Braz J Microbiol 2014;45(4):1221-7. [DOI] [PubMed]
26. Singh R, Wagh P, Wadhwani S, Gaidhani S, Kumbhar A, Bellare J, et al. Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics. Int J nanomed 2013;8:4277.
27. Shanthi S, Jayaseelan BD, Velusamy P, Vijayakumar S, Chih CT, Vaseeharan B. Biosynthesis of silver nanoparticles using a probiotic Bacillus licheniformis Dahb1 and their antibiofilm activity and toxicity effects in Ceriodaphnia cornuta. Microb pathog 2016;93:70-7. [DOI] [PubMed]
28. Sadhasivam S, Shanmugam P, Yun K. Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Colloids Surf B Biointerfaces 2010;81(1):358-62. [DOI] [PubMed]
29. Sadowski Z. Biosynthesis and application of silver and gold nanoparticles. In: Perez DP (Ed). Silver Nanoparticles. 4th. Croatia: InTech; 2010. p. 342. [DOI]
30. Singh R, Shedbalkar UU, Wadhwani SA, Chopade BA. Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl microbiol biotechnol 2015;99(11):4579-93. [DOI] [PubMed]
31. Paul D, Sinha SN. Extracellular Synthesis of Silver Nanoparticles Using Pseudomonas aeruginosa KUPSB12 and Its Antibacterial Activity. Jordan J Biol Sci 2014;7(4):245-50. [DOI]
32. Vala A, Shah S. Rapid synthesis of silver nanoparticles by a marine-derived fungus Aspergillus niger and their antimicrobial potentials. Int J Nanosci Nanotechnol 2012;8(4):197-206.
33. Priyadarshini S, Gopinath V, Priyadharsshini NM, MubarakAli D, Velusamy P. Synthesis of anisotropic silver nanoparticles using novel strain, Bacillus flexus and its biomedical application. Colloids Surf B Biointerfaces 2013;102:232-7. [DOI] [PubMed]

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