year 12, Issue 1 (March - April 2018)                   Iran J Med Microbiol 2018, 12(1): 23-32 | Back to browse issues page

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Noorani T, Badiee S H, Manafi S, Yahyaei B. Assessment of Gold Nanoparticles Cytotoxicity Produced by the Biological Method by Fusarium Oxysporum on Normal and Cancerous Fibroblast Cell Lines. Iran J Med Microbiol 2018; 12 (1) :23-32
1- Department of Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2- Department of Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran ,
3- Department of Basic Science, Faculty of Medical, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Abstract:   (6041 Views)
Background and Aims: Although there are several ways of nanoparticles production, however the biological method of nanoparticles production is under attention of researchers due to its eco friendly and energy saving properties. In the present study biosynthesis of gold nanoparticle was done by Fusarium oxysporum fungus strain and the cytotoxicity of the obtained nanoparticles was evaluated in the cell culture.
Materials and Methods: Fusarium oxysporum was purchased and cultured in Sabouraud dextrose broth medium. The culture supernatant was subjected to chloroauric acid solution at the final concentration of 1 mM. After nanoparticles production, the color changed reaction mixture was used for characterization with spectrophotometry, XRD and TEM microscopy. The obtained nanoparticles were washed and sterilized. Finally two cell lines (CIRC-HLF as normal and SW 872 as cancerous cell lines) were used for 3-(4,5 dimethyl thiazol-2yl)-2,5 diphenyl tetrazolium bromide (MTT) assay.
Results: The fungal supernatant color after 24 hours was changed from yellow to red. TEM images have shown the nanoparticles were spherical or hexagonal and their sizes were around 50-70 nanometer. MTT assay and cell culture assay showed that the produced GNPs had toxic effect when they were used at high concentrations and the CIRC-HLF cell line was more resistant than SW 872 one against the higher concentration of GNPs.
Conclusions: It seems that the biological production of gold nanoparticles by Fusarium oxysporum can be done and  can be easily produced, washed, sterilized and can be used in vivo.

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Type of Study: Original Research Article | Subject: Nanotechnology In Medicine
Received: 2017/05/3 | Accepted: 2018/02/19 | ePublished: 2018/05/14

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