year 1, Issue 1 (Spring 2007)                   Iran J Med Microbiol 2007, 1(1): 11-16 | Back to browse issues page

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Feizabadi M, Sayady S, Shokrzadeh L, Khatibi S, Gharavi S. Aminoglycosides modifying enzymes genes among the population of enterococci in Tehran. Iran J Med Microbiol. 2007; 1 (1) :11-16
1- Department of Microbiology, School of Medicine, Tehran University of Medical Science ,
2- Department of Biological Science, Alzahra University, Tehran, Iran
Abstract:   (16497 Views)
Background and objectives: Resistance to high level concentration of gentamicin is widespread among isolates of enterococci at Tehran Hospitals. To understand the mechanism of resistance among the Iranian isolates, we screened a collection of E. faecalis and E. faecium isolates to detect aminoglycoside modifying enzymes genes.
Material and Methods: To detect the high level gentamicin resistant isolates of enterococci (HLGR phenotype, MIC>500 µg/ml), 114 clinical isolates of E. faecalis(n=79) and E. faecium(n=35) were tested with disks containing 120 µg of gentamicin. The macrobroth dilution assay was then used to determine the minimum inhibitory concentration of gentamicin. The susceptibility of isolates against amikacin, netilmicin, tobramycin, kanamycin were also determined by Kirby-Bauer method. All isolates were subjected to polymerase chain reaction assays targeting aminoglycoside modifying enzyme (AMEs) genes including aac(6′)-aph(2″) , aph(2″)-Ib, aph(2″)-Ic, aph (2″)-Ia, aph (2″)-Id. aph(3')-IIIaand ant(4′)-Ia.
Results: All isolates with HLGR phenotype and those showing 64E. faeclais and E. faecium were 59.5% and 64.7% respectively. The gene aph(2″)-Ic was amplified in two
isolates of E. faecium. The results of PCR for aph (2″)-Id, ant(4′)-Ia andaph(2″)-Ib genes were negative.
Conclusion: The aac(6′)-aph(2″)was the most frequent gene encoding resistance to gentamicin and other aminoglycosides followed by  aph(3')-IIIa. Isolates lacking these genes were susceptible to all aminoglyocosides used in this study.
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Type of Study: Original | Subject: Molecular Epidemiology
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10

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