year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 178-183 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hormozi B, Rashki A, Alipoor M, Najimi M. Frequency of Pathogenic Genes fimH ،irp2 ، rmpA، allS and wcaG of Klebsiella pneumoniae Isolates by Multiplex-PCR Method. Iran J Med Microbiol. 2018; 11 (6) :178-183
1- Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2- Department of Physiopathology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran ,
3- Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
4- Department of Physiopathology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
Abstract:   (5275 Views)

Background and Aims: Klebsiella pneumoniae that causes bloodstream and urinary tract infections has several virulence factors encoding genes. Factors like the ability of adherence to the host cell surface, presence of sidrophore receptors and regulation of mucoid phenotype are the common virulence ones involved in the pathogenesis of K. pneumoniae. The aim of this study was to determine the frequency of virulence factor encoding genes in K. pneumoniae isolates, collected from hospitalized patients by Multiplex PCR.
Materials and Methods: In this descriptive study, the frequency of genes encoding virulence factor irp2, wcaG, rmpA, rmpA، allS and fimH were evaluated in 100 K. pneumoniae isolates, collected from patients referred to teaching hospitals of Zahedan city by Multiplex PCR.
Results: Among 100 clinical isolates of K. pneumoniae, allS gene had the highest frequency, 90%, and rmpA had the lowest frequency, 6%. The frequency of genes fimH, irp2, wcaG and rmpA were 88%, 87%, 33% and 6% respectively.
Conclusions: The results of this study showed that the allS, irp2 and fimH genes were more frequent among K. pneumoniae isolated from studied patients in Zahedan city. These findings provide important information on the importance of these virulence factors on pathology of urogenital tract infection, management of Klebsiella infection and success of treatment strategies.

Full-Text [PDF 672 kb]   (1103 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
Received: 2016/10/11 | Accepted: 2018/01/11 | ePublished: 2018/03/19

1. Abate G, Koh T-H, Gardner M, Siu LK. Clinical and bacteriological characteristics of Klebsiella pneumoniae causing liver abscess with less frequently observed multi-locus sequences type, ST163, from Singapore and Missouri, US. J Microbiol Immunol Infect. 2012;45(1):31-6. [DOI] [PubMed]
2. Maki DG, Tambyah PA. Engineering out the risk for infection with urinary catheters. Emerg Infect Diseases. 2001;7(2):342-7. [DOI]
3. Runcharoen C, Moradigaravand D, Blane B, Paksanont S, Thammachote J, Anun S, et al. Whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental Klebsiella pneumoniae. Genome medicine. 2017;9(1):6. [DOI] [PubMed]
4. Yan Q, Zhou M, Zou M, Liu W-e. Hypervirulent Klebsiella pneumoniae induced ventilator-associated pneumonia in mechanically ventilated patients in China. Eur J Clin Microbiol Infect Dis . 2016;35(3):387-96. [DOI] [PubMed]
5. Victor LY, Hansen DS, Ko WC, Sagnimeni A, Klugman KP, Von Gottberg A, et al. Virulence characteristics of Klebsiella and clinical manifestations of K. pneumoniae bloodstream infections. Emerging infectious diseases. 2007;13(7):986. [DOI] [PubMed]
6. Candan ED, Aksöz N. Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors. Acta Biochim Pol. 2015;62(4):867-74. [DOI] [PubMed]
7. Fang C-T, Chuang Y-P, Shun C-T, Chang S-C, Wang J-T. A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications. J Exp Med. 2004;199(5):697-705. [DOI] [PubMed]
8. Melo RdCA, de Barros EMR, Loureiro NG, de Melo HRL, Maciel MAV, Lopes ACS. Presence of fimH, mrkD, and irp2 virulence genes in KPC-2-producing Klebsiella pneumoniae isolates in Recife-PE, Brazil. Curr Microbiol. 2014;69(6):824-31. [DOI] [PubMed]
9. Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, et al. Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization. PloS one. 2009;4(3):e4982. [DOI] [PubMed]
10. Seibert G, Hörner R, Meneghetti BH, Righi RA, Forno NLFD, Salla A. Nosocomial infections by Klebsiella pneumoniae carbapenemase producing enterobacteria in a teaching hospital. Einstein (São Paulo). 2014;12(3):282-6. [DOI] [PubMed]
11. Tarchouna M, Ferjani A, Ben-Selma W, Boukadida J. Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. Int J Infect Dis. 2013;17(6):e450-e3. [DOI] [PubMed]
12. Yu W-L, Ko W-C, Cheng K-C, Lee H-C, Ke D-S, Lee C-C, et al. Association between rmpA and magA genes and clinical syndromes caused by Klebsiella pneumoniae in Taiwan. Clin Infect Dis. 2006;42(10):1351-8. [DOI] [PubMed]
13. Afrough P, Pourmand MR, Zeinalinia N, Yousefi M, Abdossamadi Z, Bagherzadeh Yazdchi S. Molecular typing of clinical and nasal carriage isolates of staphylococcus aureus by spa gene patterns. J Mazandaran Univ Med Sci. 2012;22(94):28-34.
14. Yu W-L, Lee M-F, Tang H-J, Chang M-C, Chuang Y-C. Low prevalence of rmpA and high tendency of rmpA mutation correspond to low virulence of extended spectrum β-lactamase-producing Klebsiella pneumoniae isolates. Virulence. 2015;6(2):162-72. [DOI] [PubMed]
15. Yeh K-M, Kurup A, Siu L, Koh Y, Fung C-P, Lin J-C, et al. Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan. J Clin Microbiol. 2007;45(2):466-71. [DOI] [PubMed]
16. Chuang Y-P, Fang C-T, Lai S-Y, Chang S-C, Wang J-T. Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess. Int J Infect Dis. 2006;193(5):645-54. [DOI] [PubMed]
17. Struve C, Bojer M, Nielsen EM, Hansen DS, Krogfelt KA. Investigation of the putative virulence gene magA in a worldwide collection of 495 Klebsiella isolates: magA is restricted to the gene cluster of Klebsiella pneumoniae capsule serotype K1. J Med Microbiol. 2005;54(11):1111-3. [DOI] [PubMed]
18. Yeh K-M, Chang F-Y, Fung C-P, Lin J-C, Siu L. magA is not a specific virulence gene for Klebsiella pneumoniae strains causing liver abscess but is part of the capsular polysaccharide gene cluster of K. pneumoniae serotype K1. J Med Microbiol. 2006;55(6):803-4. [DOI] [PubMed]
19. El Fertas-Aissani R, Messai Y, Alouache S, Bakour R. Virulence profiles and antibiotic susceptibility patterns of Klebsiella pneumoniae strains isolated from different clinical specimens. Pathologie Biologie. 2013;61(5):209-16. [DOI] [PubMed]
20. Decre D, Verdet C, Emirian A, et al. Emerging severe and fatal infections due to Klebsiella pneumoniae in two university hospitals in France. J Clin Microbiol. 2011 Aug;49(8):3012-3014. [DOI] [PubMed]
21. Lin J-C, Koh TH, Lee N, Fung C-P, Chang F-Y, Tsai Y-K, et al. Genotypes and virulence in serotype K2 Klebsiella pneumoniae from liver abscess and non-infectious carriers in Hong Kong, Singapore and Taiwan. Gut Pathogens. 2014;6(1):21. [DOI] [PubMed]
22. Chou H-C, Lee C-Z, Ma L-C, Fang C-T, Chang S-C, Wang J-T. Isolation of a chromosomal region of Klebsiella pneumoniae associated with allantoin metabolism and liver infection. Infection and immunity. 2004;72(7):3783-92. [DOI] [PubMed]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc