The importance of molecular techniques in identification and phylogenetic studies of Non Tuberculosis Mycobacteria

keikha, masoud

year 10, Issue 6 (January - February 2017) Iran J Med Microbiol 2017, 10(6): 78-81 | Back to browse issues page

Mendeley

Zotero

RefWorks

keikha M. The importance of molecular techniques in identification and phylogenetic studies of Non Tuberculosis Mycobacteria. Iran J Med Microbiol 2017; 10 (6) :78-81
URL: http://ijmm.ir/article-1-614-en.html

The importance of molecular techniques in identification and phylogenetic studies of Non Tuberculosis Mycobacteria

Masoud Keikha

Department of Medical Microbiology, Isfahan Medical School, Isfahan, Iran , masoud.keykha90@gmail.com

Abstract: (7886 Views)

Due to immune deficiency diseases (cancer, transplant recipients and HIV) environmental mycobacteria infections are increasing. Forasmuch as the clinical symptons of environmental mycobacteria lung infections and tuberculosis are similar, due to difference of diet therapy between environmental mycobacterium infections and Mycobacterium tuberculosis, it is necessary to identify and differentiate Mycobacterium species. Molecular methods seem to be a useful tool for detection and differentiation of Mycobacterium species.

Keywords: Nontuberculosis Mycobacteria, hsp65, rpoB, 16S rRNA

Full-Text [PDF 383 kb] (2741 Downloads)

Type of Study: Letter to the Editor | Subject: Molecular Microbiology
Received: 2016/09/4 | Accepted: 2016/11/21 | ePublished: 2017/02/5

References

1. Falsafi S, Bostanabad SZ, Feizabadi MM, Ali R, Khavari-Nejad AS, Sheikhi N, et al. Isolation and Molecular Identification of Mycobacterium fortuitum isolates from Environmental water and clinical samples at different regions of Iran. Bull Env Pharmacol Life Sci 2015;4:63-8. [Article]

2. Velayati AA, Farnia P, Mozafari M, Malekshahian D, Seif S, Rahideh S, et al. Molecular epidemiology of nontuberculous mycobacteria isolates from clinical and environmental sources of a metropolitan city. PLoS ONE 2014;9(12):e114428. [PubMed]

3. Heidarieh P, Mirsaeidi M, Hashemzadeh M, Feizabadi MM, Bostanabad SZ, Nobar MG, et al. In Vitro Antimicrobial Susceptibility of Nontuberculous Mycobacteria in Iran. Microb Drug Resist 2016;22(2):172-8. [PubMed]

4. Heidarieh P, Shojaei H, Feizabadi MM, Havaei A, Hashemi A, Ataei B, et al. Molecular identification and conventional susceptibility testing of Iranian clinical Mycobacterium fortuitum isolates. Iran J Basic Med Sci 2010;13(1):210-5. [Article]

5. Shamaei M, Marjani M, Farnia P, Tabarsi P, Mansouri D. Human infections due to Mycobacterium lentiflavum: first report in Iran. Iran J Microbiol 2010;2(1):29-31. [PubMed]

6. Hashemi-Shahraki A, Darban-Sarokhalil D, Heidarieh P, Feizabadi MM, Deshmir-Salameh S, Khazaee S, et al. Mycobacterium simiae: a possible emerging pathogen in Iran. Jpn J Infect Dis 2013;66(6):475-9. [PubMed]

7. Esfahani BN, Yazdi HR, Moghim S, Safaei HG, Esfahani HZ. Rapid and accurate identification of Mycobacterium tuberculosis complex and common non-tuberculous mycobacteria by multiplex real-time PCR targeting different housekeeping genes. Curr Microbiol 2012;65(5):493-9. [PubMed]

8. Adékambi T, Berger P, Raoult D, Drancourt M. rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol 2006;56(1):133-43. [PubMed]

9. Dai J, Chen Y, Dean S, Morris JG, Salfinger M, Johnson JA, et al. Multiple-genome comparison reveals new loci for Mycobacterium species identification. J Clin Microbiol 2011;49(1):144-53. [PubMed]

10. Ringuet H, Akoua-Koffi C, Honore S, Varnerot A, Vincent V, Berche P, et al. hsp65 sequencing for identification of rapidly growing mycobacteria. J Clin Microbiol 1999;37(3):852-7. [PubMed]

11. Adékambi T, Drancourt M, Raoult D. The rpoB gene as a tool for clinical microbiologists. Trends Microbiol 2009;17(1):37-45. [PubMed]

12. Vos M, Quince C, Pijl AS, de Hollander M, Kowalchuk GA. A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity. PLoS ONE 2012; 7(2):e30600. [Article]