year 13, Issue 4 (September - October 2019)                   Iran J Med Microbiol 2019, 13(4): 294-301 | Back to browse issues page


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Mokhtari K, Amini K. Identification of virulence genes in Pseudomonas aeruginosa isolated from human and animal samples by multiplex-PCR and their antibiotic resistance pattern. Iran J Med Microbiol. 2019; 13 (4) :294-301
URL: http://ijmm.ir/article-1-602-en.html
1- MSc in Microbiology, Faculty of Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Associate Professor, Faculty of Life Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com
Abstract:   (4076 Views)

Background and Aims: A major problem in the treatment of Pseudomonas aeruginosa infections is the emergence of strains with multiple resistances (MDR). The aim of this study was to identify virulence genes lasB, toxA, algD, exos in P. aeruginosa isolated from human and animal using Multiplex-PCR method and determination of antibiotic resistance.
Materials and Methods: This cross-sectional study was performed on the 120 non-repetitive samples, including, 60 clinical samples of human and 60 animal samples collected from Tehran, Iran. Antibiotic susceptibility test was performed by disk diffusion test. The multiplex - PCR method was performed to identify various virulence genes.
Results & Conclusion: The highest resistance rate was related to amoxicillin and amikacin in the both types of samples. lasB and exoA genes were the most prevalent virulence determinants in the human and animals samples, respectively. The results of antibiotic susceptibility test showed that the resistance in human strains was far higher than animal and this could be the result of arbitrary administration of the drug. The lasB gene in human specimens and the exoA gene in animals play an important role in the development of the disease.

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Type of Study: Brief report | Subject: Medical Bacteriology
Received: 2016/08/8 | Accepted: 2019/09/4 | ePublished: 2020/01/19

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