year 10, Issue 3 (July - August 2016)                   Iran J Med Microbiol 2016, 10(3): 73-78 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amini K, Mobasseri P, Mokhtari A. Detection of blaPSE and blaTEM genes encoding B-Lactamase in clinical samples of Salmonella typhimurium by Multiplex PCR. Iran J Med Microbiol 2016; 10 (3) :73-78
1- Department of Microbiology, Faculty of Basic Science, Saveh Branch, Islamic Azad University, Saveh, Iran ,
2- Department of Microbiology, Faculty of Biology sciences, Tehran north Branch, Islamic Azad University, Tehran, Iran
3- Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (8183 Views)

Background and Aim: B-Lactam antibiotics are widely used in the treatment of salmonellosis. Alarming reports have pointed out the rapid development of resistance to these agents, involving Salmonella serovars such as Enteritidis, Typhimurium, Panama, and Typhi in several countries. The aim of this study was to investigate the antibiotic resistance profiles and the prevalence of blaPSE and blaTEM genes among Salmonella typhimurium in humans in Tehran.

Materials and Methods: All 46 isolates were detected from a collection of human sampels in veterinary faculty, Islamic Azad University. Samples were tested to resist to ampicillin, chloramphenicol, tetracycline, cephalothin, ceftriaxone, ceftazidime, amoxicillin-clavulanate, gentamicin, trimethoprim-sulfamethoxazole and amikacin. Isolates examined by Polymerase chain reaction analysis to detect the presence of the blaPSE and blaTEM resistance genes.

Results  and Conclusions: Results of antibiogram showed (82.6%) resistance to ampicillin, (80.4%) to chloramphenicol, (69.5%) to tetracycline, (80.4%) to cephalothin, (56.5%) to amoxicillin-clavulanate and (43.4%) to trimethoprim-sulfamethoxazole. Among 46 Salmonella isolates, 43 (93.4%) showed resistance to two or more antibiotic families. blaTEM gene were identified in 1 (2.1%) Salmonella isolates. All the isolates were negative for blaPSE gene. Salmonella strains among humans were resistant to ampicillin, cephalothin, chloramphenicol, tetracycline, amoxicillin-clavulanic acid and trimethoprim-sulfamethoxazole.The identification of ESBL genes in Salmonella and MDR Multi Drug Resistance (93.4%) has considerable implications for public health. Carrying two or more beta-lactamase resistance gene is very worring, because certain combinations of genes could effectively limit all β-lactam therapeutic options. Besides, ESBL producing bacteria are typically associated with multidrug resistance

Full-Text [PDF 620 kb]   (2529 Downloads)    
Type of Study: Brief Original Article | Subject: Molecular Microbiology
Received: 2015/10/8 | Accepted: 2016/02/12 | ePublished: 2016/10/16

1. Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ. 2004; 82 (5): 346-53. [PubMed]
2. Parry CM. Antimicrobial drug resistance in Salmonella enterica. Curr Opin Infec Dis. 2003; 16 (5): 467-72. [PubMed]
3. Paterson DL, Bonomo RA. Extended-spectrum β-lactamases: a clinical update. Clin Microbiol Rev 2005; 18(4): 657–86. [PubMed]
4. Cheung TKM, Chu YWC, Chu MY, Choi HM, Yung RWHY, Kam KM. Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype enteritidis in Hong Kong. J Antimicrob Chemother. 2005; 56: 586-89. [PubMed]
5. Huovinen Pentti, Jacoby GA. Sequence of the PSE-1 β-Lactamase Gene. Antimicrob. Agents Chemother. 1991: 35(11): 2428-2430. [PubMed]
6. Washington W, Allen S, Janda W, Koneman. E, Procop G, Schreckenberger P, Woods G. Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Philadelphia: Lippincott Williams and Wilkins Press; 2002. [Article]
7. Clinical and Laboratory Standards Institute (CLSI). (2011) Performance Standards for Antimicrobial Susceptibility Testing. 21th Information Supplement. M100-S21. CLSI, Wayne, PA.
8. Li R, Lai J, Wang Y, Liu Sh, Li Y, Liu K, Shen J, Wu C. Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China. Int J Food Microbiol. 2013; 163 (1): 14-18. [PubMed]
9. Budak F, Nordmann P, Girlich D, Gür D. Characterization of extended-spectrum ß-lactamase-producing Salmonella isolates in a children’s hospital in Ankara- first report of SHV-2a and SHV-9 in Salmonella spp. from Turkey. Turk J Pediatr. 2009; 51(1): 28-34. [PubMed]
10. Gautam K, Pokhrel BM, Bhatta DR, Shrestha CD. Studies on Extended Spectrum Beta Lactamase (ESBL) producing salmonella isolates from clinical samples of Nepal. Nepal Med Coll J. 2012; 14(3): 204-206. [PubMed]
11. Su LH, Chiu CH, Chu C, Wang MH, Chia JH, Wu TL. In vivo acquisition of ceftriaxone resistance in Salmonella enterica serotype anatum. Antimicrob Agents Chemother. 2003; 47(2): 563-7. [PubMed]
12. Jacoby GA, Medeiros AA. More extended-spectrum beta-lactamases. Antimicrob Agents Chemother.1991; 35(9): 1697-704. [PubMed]
13. Sturenburg E, Mack D. Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control. J Infect. 2003; 47(4): 273-95. [PubMed]
14. González-López JJ, Piedra-Carrasco N, Salvador F, Rodríguez V, Sánchez-Montalvá A, Planes AM, ESBL-Producing Salmonella enterica Serovar Typhi in Traveler Returning from Guatemala to SpainEmerg Infect Dis. 2014; 20(11):1918-20. [PubMed]
15. Elumalai S, Muthu G, Esther Mary Selvam R, Ramesh S. Detection of TEM-,SHV- and CTX-M-type b-lactamase production among clinical isolates of Salmonella species. J Med Microbiol. 2014; 63(Pt 7): 962–967. [PubMed]
16. Gebreyes WA, Thakur Siddhartha. Multidrug-Resistant Salmonella enterica Serovar Muenchen from Pigs and Humans and Potential Interserovar Transfer of Antimicrobial Resistance. Antimicrob Agents Chemother. 2005; 49(2): 503-511.
17. Zou M, Keelara Sh, Thakur S. Molecular Characterization of Salmonella enterica Serotype Enteritidis Isolates from Humans by Antimicrobial Resistance, Virulence Genes, and Pulsed-Field Gel Electrophoresis. Foodborne Pathog Dis. 2012; 9(3): 232-238. [PubMed]
18. Tajbakhsh M, Hendriksen RS, Nochi Z, Zali MR, Aarestrup FM, Garcia-Migura L. Antimicrobial resistance in Salmonella spp. recovered from patients admitted to six different hospitals in Tehran, Iran from 2007 to 2008. Folia Microbiol. 2012; 57(2): 91-97. [PubMed]
19. ChumaT, MiyasakoD, Dahshan H, TakayamaT, NakamotoY, Shahada F, Akiba M, Okamoto K. Chronological change of resistance to β-lactams in Salmonella enterica serova rInfantis isolated from broilers in Japan. Front Microbiol. 2013; 4:113.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc