year 6, Issue 4 (Winter 2013)                   Iran J Med Microbiol 2013, 6(4): 72-80 | Back to browse issues page

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Molla-abbaszadeh H, Mobayen H, Mirzaei H. Identification of PantonValentine Leukocidin (pvl) Genes in Staphylococcus aureusisolated from In- patients of Emam Reza and Shohada Hospitals of Tabriz by Real-Time PCR . Iran J Med Microbiol. 2013; 6 (4) :72-80
URL: http://ijmm.ir/article-1-49-en.html
1- Department of Microbiology, Zanjan Branch, Faculty of Basic and Medical Sciences, Islamic Azad University, Zanjan, Iran
2- Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz , Iran , drhmobaiyen@iaut.ac.ir
3- Department of Food hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Abstract:   (10664 Views)
Background & objectives: Staphylococcus aureus could produce different toxins such as alpha, beta, and gamma-toxins and leukocidin Penton - Valentine. Leukocidin (PVL) is a cellular toxin which acts against polymorphonuclear cells, monocytes, and macrophages and causes an increase in cellular membrane permeability and leukocytes lysis and tissue necrosis. This study intends to determine the prevalence of PVL positive strainsof Staphylococcus aureus by Real-Time PCR method.
Material & Methods: This descriptive study was done during the last 6 months of the 2011. 47 Staphylococcus aureus strains from the clinical samples of Imam Reza Hospital and 53 strains from Shohada Hospital were collected from Tabriz- Iran. Real-Time PCR method was done by using specific primers and probes.
Results: From 100 Staphylococcus aureus isolates, 18 strains (18%) were PVL positive. Eleven strains belonged to Imam Reza Hospital and 7 strains to Shohada Hospital isolates.
Conclusion: By regard to producing PVL-toxins in Staphylococcus aureus strains accounts as a serious threat for human health. So rapid and accurate diagnosis of this gene in these bacteria is necessary. Therefore, it seems that access a rapid and repeatable method will help in rapid diagnosis and timely control of PVL- producing strains in clinical centers
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Type of Study: Original | Subject: Medical Bacteriology
Received: 2013/11/6 | Accepted: 2013/11/11 | ePublished: 2013/11/11

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