year 6, Issue 4 (Winter 2013)
Iran J Med Microbiol 2013, 6(4): 59-71 |
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Gharahkhani M, Khaki P. Evaluation of Extended-Spectrum beta-Lactamases ProducingIsolates of Salmonella spp.from Different Sources Using by Phenotypic and Genotypic Methods . Iran J Med Microbiol 2013; 6 (4) :59-71
URL: http://ijmm.ir/article-1-48-en.html
URL: http://ijmm.ir/article-1-48-en.html
1- Department of Science, Science and Research BranchIslamic Azad University Tehran, Iran
2- Department of Microbiology Razi of Vaccines and Serum Research, Karaj, Iran , khakipejvak@yahoo.com
2- Department of Microbiology Razi of Vaccines and Serum Research, Karaj, Iran , khakipejvak@yahoo.com
Abstract: (12805 Views)
Background and Objectives: Salmonella spp. are important zoonotic pathogens in humans and
animals which cause salmonellosis. Use of uncontrolled antimicrobial agents in human and
animal have been increased drug resistance in the different serotypes of Salmonella. The aim of
this study was determining antibiotic resistant patterns and detection of ESBL producing
isolates of Salmonellaespp. Isolated from different sources including human, poultry and cattle.
Materials and Methods: A total of 70 salmonellaisolates which obtained from Microbial Type Culture Collection of Microbiology department of Razi institute, Karaj-Iran, were subjected to determine of susceptibility testing to 30 antimicrobial agents. Phenotypic identification for ESBL producing isolates were analyzed by combined disk test (CT) according to CLSI guidelines. Genotypic characterization of isolates was performed by PCR based on bla SHVgene.
Results: (84.3%) of the Salmonellaisolates were resistant to more than one antimicrobial agent. Thirty-two (45.7%) isolates was found to be multi-drug resistant (MDR) isolates that two of them were resistant to 13 antibiotics. The most common resistance to antibiotics was to cephalexin followed by to nitrofuratoin and tetracycline. No resistance was observed for amikacin, oflaxacin, enrofloxacin, cefotizoxim, cefotaxime, and imipenem. The results revealed that 10 isolates (14.3%) were ESBL producing isolates by combined disk test while only 6 isolates(8.6%) harbored the bla SHVgene.
Conclusion:The results of this study showed the presence of resistance gene of bla SHV in different Salmonellaspp. Isolated from livestock and poultry in Iran. Therefore the continuing monitoring and identification of ESBL producing isolates of Salmonella in the community is essential.
Materials and Methods: A total of 70 salmonellaisolates which obtained from Microbial Type Culture Collection of Microbiology department of Razi institute, Karaj-Iran, were subjected to determine of susceptibility testing to 30 antimicrobial agents. Phenotypic identification for ESBL producing isolates were analyzed by combined disk test (CT) according to CLSI guidelines. Genotypic characterization of isolates was performed by PCR based on bla SHVgene.
Results: (84.3%) of the Salmonellaisolates were resistant to more than one antimicrobial agent. Thirty-two (45.7%) isolates was found to be multi-drug resistant (MDR) isolates that two of them were resistant to 13 antibiotics. The most common resistance to antibiotics was to cephalexin followed by to nitrofuratoin and tetracycline. No resistance was observed for amikacin, oflaxacin, enrofloxacin, cefotizoxim, cefotaxime, and imipenem. The results revealed that 10 isolates (14.3%) were ESBL producing isolates by combined disk test while only 6 isolates(8.6%) harbored the bla SHVgene.
Conclusion:The results of this study showed the presence of resistance gene of bla SHV in different Salmonellaspp. Isolated from livestock and poultry in Iran. Therefore the continuing monitoring and identification of ESBL producing isolates of Salmonella in the community is essential.
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2013/11/6 | Accepted: 2013/11/11 | ePublished: 2013/11/11
Received: 2013/11/6 | Accepted: 2013/11/11 | ePublished: 2013/11/11
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