year 10, Issue 4 (September - October 2016)                   Iran J Med Microbiol 2016, 10(4): 17-24 | Back to browse issues page

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Hosseini S, Ranjbar R, Zahrayi Salehi T. The Identification of pathogenic Escherichia coli strains to carry intimin gene isolated from various water sources. Iran J Med Microbiol. 2016; 10 (4) :17-24
1- Department of Microbiology, faculty of science, Islamic Azad University, Save Branch, Save, Iran
2- Molecular Biology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran ,
3- Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
Abstract:   (9811 Views)

Background and Aim: Escherichia coli  is considered  as  one  of  the  most  important  causes  of   bacterial    infections   transmitted   through   water   and  foods. Several factors are involved in the pathogenesis of Escherichia coli  .  The aim  of  this  study  was  to   investigate  the  prevalence of Escherichia coli  strains carrying  virulence gene  intimin isolated from different water sources in  Alborz province.

Materials and Methods: This study was carried  out in 2013.   The  research  included   all  E. coli  strains isolated from different surface water sources in Alborz province. Escherichia coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for the presence of intimin gene by  PCR using specific primers.

Results: One hundred E. coli  strains  were isolated  then PCR was successfully optimized for detection of intimin gene. The  PCR  results  showed  33%  of  the  strains carried out  intimin  gene.

Conclusions: Compared to many studies, our findings showed the high frequency of E. coli  strains carrying  intimin gene  isolated from surface water sources. The presence of this virulence genes is a potential threat for contamination of drinking water which is important for health care systems.

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Type of Study: Original | Subject: Molecular Microbiology
Received: 2015/04/8 | Accepted: 2015/05/25 | ePublished: 2016/10/16

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