year 10, Issue 3 (July - August 2016)                   Iran J Med Microbiol 2016, 10(3): 19-30 | Back to browse issues page

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Mortazavi S M, Hosseinzadeh Colagar A, Sohbatzadeh F. The Efficiency of the Cold Argon-oxygen Plasma jet to reduce Escherichia coli and Streptococcus pyogenes from solid and liquid ambient . Iran J Med Microbiol 2016; 10 (3) :19-30
1- Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
2- Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran ,
3- Department of Atomic and Molecular Physics, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Abstract:   (10958 Views)

Background and Aim: Bacterial infections in hospitals can be consider as a damaging factor in laboratory environment (healthcare setting) since the rate of antibiotic-resistant bacteria is increasing overtime. Therefore, finding an effective and rapid method is essential for sterilization. The aim of this study was efficiency of cold argon-oxygen plasma used for sterilization of the Escherichia coli and Streptococcus pyogenes in liquid and solid media.

Materials and Methods: Non-thermal single argon-oxygen plasma jet was produced by high sinusoidal voltage (18.56 kHZ). Then 3*108 CFU/ml of E. coli and 3*107 CFU/ml of
S. pyogenes were inoculated in LB (Luria-Bertani) liquid and solid media, separately. These inoculated media were treated with plasma for different time periods. Results were measured by turbidity test in liquid media and measuring clear zone in solid media. Moreover pieces present in the plasma and concentration of zone were measured.

Results: The results suggested that cold argon/oxygen plasma jet including atomic and molecular species, has a high efficiency to destroy gram-positive and gram-negative bacteria at short time in a liquid medium (less than 15 minutes) and solid (less than 30 second). Also, results have shown that the influence factor of gram-negative bacteria (E. coli) was more than gram-positive bacteria (S. pyogenes).

Conclusions: Since many of sterilization procedures including chemical and physical methods or wet and dry heat sterilization in solid or liquid ambient have faced to functional limitations; according to the results of this study, this new technology is a powerful tool for disinfection of surfaces in hospitals and public places.

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Type of Study: Original Research Article | Subject: Nosocomial infections
Received: 2014/12/11 | Accepted: 2016/05/4 | ePublished: 2016/10/16

1. Valipour F, Rezaee A, Salem M, Norian S, Motamed Pour L. Removal of Pseudomonas aeruginosa in hospital air using microwave radiation. Iran J Med Microbiol. 2013;7(2):8-13. [Article]
2. Mirnejad R, Fallahi S, Jeddi F, Kiani J, Taslimi R. Study of spontaneous bacterial peritonitisetiologic agents and determination of their antibiotic resistance pattern. Iran J Med Microbiol. 2007;1(2):43-8. [Article]
3. Kong MG, Kroesen G, Morfill G, Nosenko T, Shimizu T, Van Dijk J, et al. Plasma medicine: An introductory review. New J Phys. 2009; 11(11): 115012-46. [Article]
4. Waters A, Contente-Cuomo T, Buchhagen J, Liu CM, Watson L, Pearce K, et al. Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry. Clin Infect Dis. 2011;52(10):1227-30. [PubMed]
5. Rinsky J, Nadimpalli M, Wing S, Hall D, Baron D, Price L, et al. Livestock-Associated Methicillin and Multidrug Resistant Staphylococcus aureus Is Present among Industrial, Not Antibiotic-Free Livestock Operation Workers in North Carolina. PLoS ONE. 2013;8(7): e67641. [PubMed]
6. Mohammadimehr M, Feizabadi M, Bahadori O, Motshaker Arani M, Khosravi M. Study of prevalence of gram- negative bacteria caused nosocomial infections in ICU in besat hospital in Tehran and detection of their antibiotic resistance pattern-year. Iran J Med Microbiol. 2009;3(2&3):47-54. [Article]
7. Shirazi M, Akbari A, Sharifi Yazdi MK, Hosseini M, Fardsanei F, Bakhtiari R, et al. Antibiotic resistance patterns of enteropathogenic E. coli (EPEC) serogroups isolated from stool of under 5 years old children with diarrhea in Tehran:2007- 2008. Iran J Med Microbiol. 2009;2(3&4):59–65. [Article]
8. Lloyd G, Friedman G, Jafri S, Schultz G, Fridman A, Harding K. Gas Plasma: medical uses and developments in wound care. Plasma Proc Poly. 2010; 22;7 (3-4):194-211. [Article]
9. Etufugh CN, Phillips TJ. Venous ulcers. Clin Dermatol. 2007;25(1):121-30.
10. Vaez H, Ghazi Saeidi K, Moradi A, Tabaraei A, Khodabakhshi B, Bazouri M, et al. Antibiotic resistance pattern of methicillin resistant Staphylococcus aureus isolated from Health-educational centers of Gorgan, Iran, 2008-2009. Iran J Med Microbiol. 2010;3(4):31-6. [Article]
11. Dormanesh B, Mirnejad R, Khodaverdi Dariyan E, Momtaz H, Yahaghi E, Safarpour Dehkordi F, et al. Characterization and study the antibiotic resistance of uropathogenic Escherichia coli isolated from pediatrics with pyelonephritis and cystitis in Iran. Iran J Med Microbiol. 2013;7(2):27-39. [Article]
12. Kampf G, Widmer AF. Compliance with application time for surgical hand disinfection. Infect Control Hosp Epidemiol. 2006;27(9):996-8. [PubMed]
13. Fridman G, Friedman G, Gutsol A, Shekhter AB, Vasilets VN, Fridman A. Applied plasma medicine. Plasma Process Polym. 2008;5(6):503-33. [Article]
14. Venezia R, Orrico M, Houston E, Yin S-M, Naumova YY. Lethal activity of nonthermal plasma sterilization against microorganisms. Infect Control Hosp Epidemiol. 2008;29(5):430-6. [PubMed]
15. Bogaerts A, Neyts E, Gijbels R, van der Mullen J. Gas discharge plasmas and their applications. Spectrochim Acta Part B At Spectrosc. 2002;57(4):609–58. [Article]
16. Xingmin S, Yukang Y, Yanzhou S, Wang Y, Fengling P, Yuchang Q. Experimental research of inactivation effect of low-temperature plasma on bacteria. Plasma Sci Technol. 2006;8(5):569. [Article]
17. Hosseinzadeh Colagar A, Sohbatzadeh F, Mirzanejhad S, Valinataj Omran A. Sterilization of Streptococcus pyogenes by afterglow dielectric barrier discharge using O2 and CO2 working gases. Biochem Engin J. 2010;51(3): 189-93. [Article]
18. Weng C-C, Wu Y-T, Liao J-D, Kao C-Y, Chao C-C, Chang J-E, Hsu BW. Inactivation of bacteria by a mixed argon and oxygen micro-plasma as a function of exposure time. Int J Radiat Biol. 2009;85(4):362–8. [PubMed]
19. Hosseinzadeh Colagar A, Memariani H, Sohbatzadeh F, Valinataj Omran A. Nonthermal atmospheric argon plasma jet effects on Escherichia coli biomacromolecules. Appl Biochem Biotechnol. 2013;171(7):1617-29. [PubMed]
20. Levinson W, Jawetz E. Medical microbiology and immunology: Examination and board review. Appleton & Lange, 1996. [Article]
21. Hosseinzadeh Colagar A, Alavi O, Motallebi S, Sohbatzadeh F. Decontamination of Streptococcus pyogenes and Escherichia coli from solid surfaces by singlet and triplet atmospheric pressure plasma jet arrays. Arab J Sci Eng. 2016;41(6):2139-2145 [Article]
22. Sohbatzadeh F, Hosseinzadeh Colagar A, Mirzanejhad S, Motallebi S, Farhadi M, Bagheri M, et al. Design and construction of triplet atmospheric cold plasma jet for sterilization. Iran J Phys Res. 2014;13(4):363-374 [Article]
23. Sohbatzadeh F, Hossienzadeh Colagar A, Mirzanejhad S, Mahmodi S. E. coli, P. aeruginosa and B. cereus bacteria sterilization using afterglow of non-thermal plasma at atmospheric pressure. Appl Biochem Biotechnol. 2010;160(7):1978-84 [PubMed]
24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2): 248-54 [Article]
25. Green MR, Sambrook J. Molecular cloning a laboratory manual. 4th ed. Cold Spring Harbor Laboratory Press- New York; 2012. [Article]
26. Poiata A, Motrescu I, Nastuta A, Creanga DE, Popa G. Microorganism response to atmospheric pressure helium plasma DBD treatment. J Electrostat. 2010;68(2):128-31. [Article]
27. Ramona F, Antoniea P, Iuliana M, Andrei N, Dorina C, Gheorghe P. Bacteria response to non-thermal physical factors: A study on Staphylococcus aureus. Afr J Biotechnol. 2012;11(18):4234-40. [Article]
28. Alkawareek MY, Algwari QT, Laverty G., Gorman GP, Graham WG, O'Connell D, et al. Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma. PLoS ONE. 2012;7:e44289. [Article]
29. Kalghatgi S, Kelly C, Cerchar E, Torabi B, Alekseev O, Fridman A, et al. Effects of non-thermal plasma on mammalian cells. PLoS ONE. 2011;6(1):e16270. [Article]
30. Song HP, Kim B, Choe JH., Jung S, Moon SY, Choe W, et al. Evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail Listeria monocytogenes. Food Microbiol. 2009;26(4):432-6. [PubMed]
31. Yang L, Chen J, Gao J. Low temperature argon plasma sterilization effect on Pseudomonas aeruginosa and its mechanisms. J Electrostatics. 2009;67(4):646-51. [Article]
32. Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick & Adelberg's medical microbiology. 27th ed. Carroll KC, Butel J, Morse S: The McGraw-Hill Companies, Inc. New York; 2016
33. Joshi SG, Cooper M, Yost A, Paff M, Ercan UK, Fridman, G, et al. Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and membrane lipid peroxidation in Escherichia coli. Antimicrob Agents Chemother. 2011; 55(3):1053-62 [PubMed]

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