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2- Department of Gynecology, Arefian General Hospital, Urmia , Iran.
3- Department of Gynecology, Motahari University Hospital, Urmia University of Medical Sciences, Urmia, Iran.
4- Department of Mycology and Parasitology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Background
and Aim: Vulvovaginal candidiasis (VVC) is a common clinical problem
that affects most adult women at least once during their life time. Many also
have frequently recurring yeast infections (RVVC) caused by Candida albicans
and non albicans Candida species. We studied use of CHROMagar Candida
and PCR-RFLP to compare their differential ability and time consuming.
Materials
and Methods: Vaginal discharge samples of all
women with clinical signs were cultured on CHROM agar Candida medium .After an
overnight incubation at 37°C, growth coloration of Candida isolates compared
with those of standard patterns. PCR-RFLP using restriction enzyme Msp I
were used for the identification of more
Candida isolates in the level of species.
Results:
Totally
192 vaginal discharge samples were obtained from the women with vulvovaginitis
clinical signs. 90 patients had Candida vulvovaginitis, 11 case were RVVC. The Candida
species which were identified included, C.albicans (78.8%), C.glabrata
(7.7 %), C. parapsilosis (6.6%) , C. tropicalis (4.4 %) and C. krusei
(2.2 %).
Conclusions:
We
concluded that, use of PCR-RFLP is recommended for the identification of Candida
species causing VVC and RVVC in accompany with the culture based methods,
regarding to its differential power and speed.
Received: 2014/11/19 | Accepted: 2014/11/19 | ePublished: 2014/11/19
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Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
