year 9, Issue 2 (Summer 2015)                   Iran J Med Microbiol 2015, 9(2): 39-46 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Afshar H, Shams-hahfarokhi M, Razzaghi-Abyaneh M. Distribution and diversity of Alt a 1 gene in Alternaria species isolated from outdoor air of Tehran. Iran J Med Microbiol. 2015; 9 (2) :39-46
1- Tarbiat ModaresDepartment of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran University
2- Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran ,
3- Department of Mycology, Pasteur Institute of Iran
Abstract:   (10471 Views)

Background and Aim: In the present study, distribution pattern and phylogeny of Alternaria species isolated from Tehran air was determine based on the homology of Alt a1 gene.

Materials and Methods: A number of 48 Alternaria isolates belonging to  A. alternata (33.4%), A. solani (23%), A. tenuissima (23%), A. porri (16.6%), and  A. brassicicola (4%) obtained from Tehran air were cultured on Sabouraud dextrose agar. Genomic DNA was extracted from the isolates and amplified with forward and reverse specific primers of Alt a1 gene. The resultant fragments of 500 to 600 bp of genomic DNA were sequenced and compared with genomic data of NCBI gene bank to evaluate phylogenetic relationship of Alternaria species.

Results: Based on the resulting dendrogram, isolates belonging to A. alternata and A. solani were distributed in 4 clusters and those of A. brassicicola, A. porri, and A. tenuissima each in 2 clusters. Approximately, 81% of A. alternata isolates, 73% of A. solani isolates, 45.5% of A. tenuissima isolates and 12.5% of A. porri isolates showed a similarity of 99% and resided in clusters I, II, IV and VII, respectively.

Conclusions: Our results indicated that Alt a1 gene was present in all examined species and differences of sequence similarities of this gene shows the importance of species and strains of Alternaria in induction of allergenic reactions in suspected patients especially those with allergic asthma.

Full-Text [PDF 847 kb]   (2904 Downloads)    
Type of Study: Original Research Article | Subject: Medical Mycology
Received: 2014/10/19 | Accepted: 2015/01/18 | ePublished: 2015/07/4

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc