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Maulin Inggraini1 
, Noor Andryan Ilsan2 , Ni Putu Ratna Ayu Krishanti3 , Jepri Agung Priyanto4 , Reza Anindita5 , Fhasya Algina Hawatul Haq2 , Oktavia Dwi Mauria2 , Salsabila Ranmika Sari6 , Najla Salsabila Rahmadina7
, Noor Andryan Ilsan2 , Ni Putu Ratna Ayu Krishanti3 , Jepri Agung Priyanto4 , Reza Anindita5 , Fhasya Algina Hawatul Haq2 , Oktavia Dwi Mauria2 , Salsabila Ranmika Sari6 , Najla Salsabila Rahmadina7
1- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia , maulin.inggraini@stikesmitrakeluarga.ac.id
2- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
3- Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Jl. Raya Bogor KM 46, Cibinong, Bogor, West Java Province 16911, Indonesia
4- Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB University), Bogor, Dramaga, Indonesia
5- Department of Pharmacy, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
6- Department of Nursing, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
7- Department of Nutrition, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
2- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
3- Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Jl. Raya Bogor KM 46, Cibinong, Bogor, West Java Province 16911, Indonesia
4- Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB University), Bogor, Dramaga, Indonesia
5- Department of Pharmacy, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
6- Department of Nursing, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
7- Department of Nutrition, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
Abstract: (72 Views)
Background and Objective: One of the animal models for bacterial virulence testing is Omphisa fuscidentalis. The purpose of this study was to evaluate Omphisa fuscidentalis larvae as an animal model for bacterial virulence testing.
Methods: The design of this study was experimental. Bacterial samples were obtained from Mitra Keluarga Hospital, Bekasi, Indonesia. Molecular Identification of Bacteria Using Partial 16S rRNA Gene Sequencing. Identification And Susceptibility Testing Of Bacteria Using Vitek 2 Compact. The dose treatment of bacterial isolates Staphylococcus haemolyticus, Enterococcus faecalis, and Klebsiella pneumoniae 103, 104, 105 was carried out on the survivability of O. fuscidentalis larvae over 24 hours post-injection. Data analysis was descriptive quantitative.
Results: K. pneumoniae strains were resistant to more than five classes of antibiotics. The lowest survival percentage was obtained in O. fuscidentalis larvae injected with K. pneumoniae. Microscopic observation of the hemocytes of dead O. fuscidentalis larvae showed swelling and increased cell size compared to healthy hemocytes from control larvae injected with sterile phosphate-buffered saline.
Conclusion: O. fuscidentalis larvae are a promising alternative model for assessing bacterial virulence.
Keywords : Virulence, Omphisa fuscidentalis, Animal model, Multi-drug resistant, Hemocytes, Galleria mellonella
Keywords: Virulence, Omphisa fuscidentalis, Animal model, Multi-drug resistant, Hemocytes, Galleria mellonella
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2025/04/22 | Accepted: 2025/07/27 | ePublished: 2025/08/18
Received: 2025/04/22 | Accepted: 2025/07/27 | ePublished: 2025/08/18
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