The Relation Between Biofilm Formation and Alginate Production in Pseudomonas aeruginosa; An Important and Neglected Topic

Shafigh, Maryam; Izadi Amoli, Rabieh; Pournajaf, Abazar; Yahyapour, Yousef; Kaboosi, Hami

doi:10.30699/ijmm.18.5.319

year 18, Issue 5 (September - October 2024) Iran J Med Microbiol 2024, 18(5): 319-328 | Back to browse issues page

‎ 10.30699/ijmm.18.5.319

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Shafigh M, Izadi Amoli R, Pournajaf A, Yahyapour Y, Kaboosi H. The Relation Between Biofilm Formation and Alginate Production in Pseudomonas aeruginosa; An Important and Neglected Topic. Iran J Med Microbiol 2024; 18 (5) :319-328
URL: http://ijmm.ir/article-1-2474-en.html

The Relation Between Biofilm Formation and Alginate Production in Pseudomonas aeruginosa; An Important and Neglected Topic

Maryam Shafigh¹

, Rabieh Izadi Amoli¹

, Abazar Pournajaf²

, Yousef Yahyapour³

, Hami Kaboosi¹

1- Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Infectious Disease and Tropical Medicine Research Center, Health Research institute, Babol University of Medical Sciences, Babol, Iran , abazar_pournajaf@yahoo.com
3- Infectious Disease and Tropical Medicine Research Center, Health Research institute, Babol University of Medical Sciences, Babol, Iran

Abstract: (565 Views)

Background and Aims: Pseudomonas aeruginosa (P. aeruginosa) is one of the most common opportunistic bacteria causing healthcare-associated infections (HAIs). Alginate as an adhesion substance plays role in its binding to inanimate surfaces and protecting against environmental conditions. Biofilm also increases the bacterial resistance to antimicrobials and immune system. The present study was aimed to survey the antimicrobial resistance pattern, biofilm formation, and alginate production in the clinical isolates of P. aeruginosa.
Materials and Methods: In this cross-sectional study in a period of one year 90 non-duplicative P. aeruginosa were isolated from the clinical samples. Antimicrobial susceptibility testing was performed by disk diffusion method. Biofilm formation and alginate production were measured by microtiter plate and carbazole assay, respectively. Polymerase chain reaction was conducted for detection of pslA, pelA, ppyR, algD, algU and algL genes.
Results: The highest resistance rate (74.4%) was related to ceftazidime. Alginate production was found in 87.8% of the isolates, which production levels were <250 µgml^-1, 250-400 µgml^-1, and >400 µgml^-1 in 7.6%, 53.2%, and 39.2 of the isolates, respectively. The categories of biofilm formation in strains were as weak (11.1%), moderate (24.1%), and strong (64.8%). The prevalence of ppyR, algD, algU, algL, pslA and pelA genes were 100.0%, 92.2%, 86.6%, 67.7%, 85.2% and 42.5%, respectively.
Conclusion: The results highlighted an alarming trend in P. aeruginosa strains antibiotic resistance rates. A significant relationship was also observed between the alginate production and the biofilm formation. Thus, periodic monitoring, adherence to the antibiotic stewardship, avoiding arbitrary prescribing, and screening tests are unavoidable.

Keywords: Alginate, Antibiotic Resistance, Biofilm, PCR, Pseudomonas aeruginosa

Full-Text [PDF 554 kb] (162 Downloads) | | Full-Text (HTML) (92 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2024/08/17 | Accepted: 2024/11/14 | ePublished: 2024/11/30

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