year 3, Issue 2 And 3 (12-2009)
Iran J Med Microbiol 2009, 3(2 And 3): 34-39 |
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Taheri J, Rafieian N, Azimi S, Mohebbi S, Navidinia M. Investigation of disinfecting effect of AlproCid solution in dentistry. Iran J Med Microbiol 2009; 3 (2 and 3) :34-39
URL: http://ijmm.ir/article-1-155-en.html
URL: http://ijmm.ir/article-1-155-en.html
1- Department of Oral Diseases, School of Dentistry,Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Orthodentic Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- The Pediatrics Infectious Diseases Research Center, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran , m.navidinia@yahoo.com
2- Orthodentic Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- The Pediatrics Infectious Diseases Research Center, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran , m.navidinia@yahoo.com
Abstract: (15618 Views)
Back ground and Objective: AlproCid is a brand new disinfectant which is a third Alkyl amine and
ammonium with considerable effectiveness. The aim ofthis study was to evaluate of effectiveness of
AlproCid on disinfecting dental surfaces and instruments.
Material and Methods: This laboratory-based study was conducted of 30 samples which were collected before and after administration of AlproCid from the same spots on dental instruments by using sterile swabs. The samples were cultured on blood agar and chocolate agar and were incubated in 37 o C for 24 hours.The standard bacteria were then identified to species level. The AlproCid solution was incorporated into strile paper discs. The effect of AlproCid was investigated by observation of growth inhibition zone around the disc and colony counting. The data was analyzed by using SPSS version 14 and Chi-Square test for determining the significance level and alpha error was set at 0.05%.
Results: From the total samples 86% were free of any bacteria. Microbial load was significantly reduced (P<0.05) after AlproCid application on dental instruments. The colony counting for all bacteria were reduced to less than 10 5 , and the growth inhibition zone was at least 10 mm.This in vitro study showed the effectiveness of AlproCid on laboratory standard strains of E. coli, Klebsiella, Proteus, Entrococci resistant to vancomycin, and S. aureusresistant to methicillin. This effect was bactericidal.
Conclusion: AlproCid is an effective agent in decreasing bacterial contamination of dental instruments without any adverse effect on the surface of instruments. The solution could be used for easy and rapid disinfecting of all surfaces
Material and Methods: This laboratory-based study was conducted of 30 samples which were collected before and after administration of AlproCid from the same spots on dental instruments by using sterile swabs. The samples were cultured on blood agar and chocolate agar and were incubated in 37 o C for 24 hours.The standard bacteria were then identified to species level. The AlproCid solution was incorporated into strile paper discs. The effect of AlproCid was investigated by observation of growth inhibition zone around the disc and colony counting. The data was analyzed by using SPSS version 14 and Chi-Square test for determining the significance level and alpha error was set at 0.05%.
Results: From the total samples 86% were free of any bacteria. Microbial load was significantly reduced (P<0.05) after AlproCid application on dental instruments. The colony counting for all bacteria were reduced to less than 10 5 , and the growth inhibition zone was at least 10 mm.This in vitro study showed the effectiveness of AlproCid on laboratory standard strains of E. coli, Klebsiella, Proteus, Entrococci resistant to vancomycin, and S. aureusresistant to methicillin. This effect was bactericidal.
Conclusion: AlproCid is an effective agent in decreasing bacterial contamination of dental instruments without any adverse effect on the surface of instruments. The solution could be used for easy and rapid disinfecting of all surfaces
Type of Study: Original Research Article |
Subject:
Antimicrobial Substances
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
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