سال 19، شماره 1 - ( بهمن - اسفند 1403 )
جلد 19 شماره 1 صفحات 4-4 |
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Mohammadjani M, Harzandi N, Haddadi A. Listeriolysin O Gene-Based Detection and Phylogenetic Investigation of Listeria monocytogenes in Food and Environmental Samples. Iran J Med Microbiol 2025; 19 (1) :4-4
URL: http://ijmm.ir/article-1-2479-fa.html
URL: http://ijmm.ir/article-1-2479-fa.html
Listeriolysin O Gene-Based Detection and Phylogenetic Investigation of Listeria monocytogenes in Food and Environmental Samples. مجله میکروب شناسی پزشکی ایران. 1403; 19 (1) :4-4
چکیده: (164 مشاهده)
Background & Objective: Listeria (L.) monocytogenes is a foodborne pathogen found in various environments including water, soil, and food. Due to the high importance of this bacterium in public health and the potential differences between its lineages in pathogenicity, this study aimed to investigate and identify the phylogenetics of Listeria in food and environmental samples based on Listeriolysin O gene.
Methods: About 100 samples were collected from Shahriar and Andisheh counties, Iran in summer and fall. After the enrichment and cultivation on selective agar, the suspicious colonies were purified. Lineage identification was performed using mismatch amplification mutation assay-polymerase chain reaction (MAMA-PCR) specifically for Listeriolysin O gene. Data were analyzed using GraphPad Prism.
Results: Of 100 samples examined, 22 samples (22%) showed contamination with L. monocytogenes. Of these, 16 (72.7%) belonged to lineage II, one (4.5%) belonged to lineage III, and the remaining 5 (22.7%) did not belong to any of the three lineages studied. Moreover, three positive samples were related to the environmental samples (water and soil).
Conclusion: The study showed lineage II as the most common lineage in the examined samples. Moreover, the results emphasized the importance of monitoring L. monocytogenes contamination in food and environment to improve control strategies and ensure food safety.
نوع مطالعه: مقاله پژوهشی |
موضوع مقاله:
باکتری شناسی پزشکی
دریافت: 1403/10/8 | پذیرش: 1403/12/14 | انتشار الکترونیک: 1404/1/10
دریافت: 1403/10/8 | پذیرش: 1403/12/14 | انتشار الکترونیک: 1404/1/10
فهرست منابع
1. Radoshevich L, Cossart P. Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis. Nat Rev Microbiol. 2018;16(1):32-46. [DOI:10.1038/nrmicro.2017.126] [PMID]
2. Amajoud N, Leclercq A, Soriano JM, Bracq-Dieye H, El Maadoudi M, Senhaji NS, et al. Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Control. 2018;84:436-41. [DOI:10.1016/j.foodcont.2017.08.023]
3. Terentjeva M, Šteingolde Ž, Meistere I, Elferts D, Avsejenko J, Streikiša M, et al. Prevalence, genetic diversity and factors associated with distribution of Listeria monocytogenes and other Listeria spp. in cattle farms in Latvia. Pathogens. 2021;10(7):851. [DOI:10.3390/pathogens10070851] [PMID] [PMCID]
4. Disson O, Moura A, Lecuit M. Making sense of the biodiversity and virulence of Listeria monocytogenes. Trends Microbiol. 2021;29(9):811-22. [DOI:10.1016/j.tim.2021.01.008] [PMID]
5. Carroll KC, Detrick B, Hobden JA, Mietzner TA, Mitchell TG, Morse SA, et al. Jawetz, Melnick, & Adelberg's Medical Microbiology (28th edition - 2019). Weitz M, Thomas CM, editors. 2019. New York, United States: McGraw-Hill Education LLC.
6. Silva NF, Neves MM, Magalhães JM, Freire C, Delerue-Matos C. Emerging electrochemical biosensing approaches for detection of Listeria monocytogenes in food samples: An overview. Trends Food Sci Technol. 2020;99:621-33. [DOI:10.1016/j.tifs.2020.03.031]
7. Quereda JJ, Leclercq A, Moura A, Vales G, Gómez-Martín Á, García-Muñoz Á, et al. Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep. Int J Syst Evol Microbiol. 2020;70(11):5868-79. [DOI:10.1099/ijsem.0.004494] [PMID]
8. Li R, Liang Q, Tian S, Zhang Y, Liu S, Ou Q, et al. Hemolysin function of Listeria is related to biofilm formation: transcriptomics analysis. Vet Res. 2022;53(1):113. [DOI:10.1186/s13567-022-01124-y] [PMID] [PMCID]
9. Vázquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Domı́nguez-Bernal G, Goebel W, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev. 2001;14(3):584-640. [DOI:10.1128/CMR.14.3.584-640.2001] [PMID] [PMCID]
10. Jordan K, McAuliffe O. Listeria monocytogenes in foods. Adv Food Nutr Res. 2018;86:181-213. [DOI:10.1016/bs.afnr.2018.02.006] [PMID]
11. Ikhimiukor OO, Mingle L, Wirth SE, Mendez-Vallellanes DV, Hoyt H, Musser KA, Wolfgang WJ, Andam CP. Long-term persistence of diverse clones shapes the transmission landscape of invasive Listeria monocytogenes. Genome Med. 2024;16(1):109. [DOI:10.1186/s13073-024-01379-4] [PMID] [PMCID]
12. Osek J, Wieczorek K. Why does Listeria monocytogenes survive in food and food-production environments?. J Vet Res. 2023;67(4):537-44. [DOI:10.2478/jvetres-2023-0068] [PMID] [PMCID]
13. van de Merwe C, Simpson DJ, Qiao N, Otto SJ, Kovacevic J, Gänzle MG, et al. Is the persistence of Listeria monocytogenes in food processing facilities and its resistance to pathogen intervention linked to its phylogeny?. Appl Environ Microbiol. 2024;90(6):e00861-24. [DOI:10.1128/aem.00861-24] [PMID] [PMCID]
14. Jinneman KC, Hill WE. Listeria monocytogenes lineage group classification by MAMA-PCR of the listeriolysin gene. Curr Microbiol. 2001;43:129-33. [DOI:10.1007/s002840010274] [PMID]
15. Wiedmann M. Molecular subtyping methods for Listeria monocytogenes. J AOAC Int. 2002;85(2):524-32. [DOI:10.1093/jaoac/85.2.524]
16. Bai X, Wang Z, Li W, Xiao F, Huang J, Xu Q, et al. Rapid and accurate detection for Listeria monocytogenes in milk using ampicillin-mediated magnetic separation coupled with quantitative real-time PCR. Microchem J. 2022;183:108063. [DOI:10.1016/j.microc.2022.108063]
17. Osek J, Lachtara B, Wieczorek K. Listeria monocytogenes in foods-From culture identification to whole‐genome characteristics. Food Sci Nutr. 2022;10(9):2825-54. [DOI:10.1002/fsn3.2910] [PMID] [PMCID]
18. Rip D, Gouws PA. PCR-restriction fragment length polymorphism and pulsed-field gel electrophoresis characterization of listeria monocytogenes isolates from ready-to-eat foods, the food processing environment, and clinical samples in South Africa. J Food Prot. 2020;83(3):518-33. [DOI:10.4315/0362-028X.JFP-19-301] [PMID]
19. Deekshit VK, Jazeela K, Chakraborty G, Rohit A, Chakraborty A, Karunasagar I. Mismatch amplification mutation assay-polymerase chain reaction: A method of detecting fluoroquinolone resistance mechanism in bacterial pathogens. Indian J Med Res. 2019;149(2):146-50. [DOI:10.4103/ijmr.IJMR_2091_17] [PMID] [PMCID]
20. Zhang F, Liu ZY, Liu S, Zhang WG, Wang BB, Li CL, et al. Rapid screening of point mutations by mismatch amplification mutation assay PCR. Appl Microbiol. Biotechnol. 2024;108(1):190. [DOI:10.1007/s00253-024-13036-2] [PMID] [PMCID]
21. Linke K, Rückerl I, Brugger K, Karpiskova R, Walland J, Muri-Klinger S, et al. Reservoirs of Listeria species in three environmental ecosystems. Appl Environ Microbiol. 2014;80(18):5583-92. [DOI:10.1128/AEM.01018-14] [PMID] [PMCID]
22. Ntshanka Z, Ekundayo TC, du Plessis EM, Korsten L, Okoh AI. Occurrence and molecular characterization of multidrug-resistant vegetable-borne Listeria monocytogenes isolates. Antibiotics. 2022;11(10):1353. [DOI:10.3390/antibiotics11101353] [PMID] [PMCID]
23. Duze ST, Marimani M, Patel M. Tolerance of Listeria monocytogenes to biocides used in food processing environments. Food Microbiol. 2021;97:103758. [DOI:10.1016/j.fm.2021.103758] [PMID]
24. Pirone-Davies C, Chen Y, Pightling A, Ryan G, Wang Y, Yao K, et al. Genes significantly associated with lineage II food isolates of Listeria monocytogenes. BMC Genom. 2018;19:1-11. [DOI:10.1186/s12864-018-5074-2] [PMID] [PMCID]
25. Quereda JJ, Morón-García A, Palacios-Gorba C, Dessaux C, García-del Portillo F, Pucciarelli MG, et al. Pathogenicity and virulence of Listeria monocytogenes: A trip from environmental to medical microbiology. Virulence. 2021;12(1):2509-45. [DOI:10.1080/21505594.2021.1975526] [PMID] [PMCID]
26. Marquis H, Drevets DA, Bronze MS, Kathariou S, Golos TG, Iruretagoyena JI. Pathogenesis of Listeria monocytogenes in Humans. In Human Emerging and Re-emerging Infections, Singh SK (Ed.).2015. pp. 749-72. New York, United States: John Wiley & Sons, Inc. [DOI:10.1002/9781118644843.ch40]
27. Jashari B, Capitaine K, Bisha B, Stessl B, Blagoevska K, Cana A, et al. Molecular characterization of Listeria monocytogenes in the food chain of the Republic of Kosovo from 2016 to 2022. Foods. 2024;13(18):2883. [DOI:10.3390/foods13182883] [PMID] [PMCID]
28. Kayode AJ, Okoh AI. Assessment of multidrug-resistant Listeria monocytogenes in milk and milk product and One Health perspective. PLoS One. 2022;17(7):e0270993. [DOI:10.1371/journal.pone.0270993] [PMID] [PMCID]
29. Najafi N, Shrifi SM, Bozorgi MA. Evaluation of listeria monocytogenes contamination of raw milk and traditional butter purchased in Amol city and detection of antibacterial resistance of isolates. Int J Food Microbiol. 2023;9(4):100-7.
30. Kester CT, Daramola JA, Alao FO, Chukwu CB. Listeria species and occurrence in retailed smoked fish samples in selected Ota markets, Ogun State, Nigeria. J Res For Wildl Environ. 2024;16(3):61-9.
31. Iacumin L, Cappellari G, Pellegrini M, Basso M, Comi G. Analysis of the bioprotective potential of different lactic acid bacteria against Listeria monocytogenes in cold-smoked sea bass, a new product packaged under vacuum and stored at 6±2 C. Front Microbiol. 2021;12:796655. [DOI:10.3389/fmicb.2021.796655] [PMID] [PMCID]
32. Babazadeh Naseri A, Soltan Dallal MM. Frequency, antimicrobial susceptibility and serotyping of Listeria monocytogenes isolated from food samples in Tehran, Iran. J Gorgan Univ Med Sci. 2019;21(1):101-7.
33. Khalafalla FA, Ali HM, El-Fouley A. Microbiological evaluation of chicken meat products. J Vet Med Res. 2019;26(2):151-63. [DOI:10.21608/jvmr.2019.66094]
34. Akbarpour S, Bahador N. Seromolecular typing of Listeria monocytogenes isolated from raw and frozen chicken nuggets. J Appl Microbiol. 2020;6(3):36-52.
35. Tahir R, Rabbani M, Ahmad A, Tipu MY, Chaudhary MH, Jayarao BM. Study on Occurrence of Listeria Monocytogenes in Soil of Punjab Province and Its Associated Risk Factors. J Anim Plant Sci. 2022;32(1):45-51. [DOI:10.36899/JAPS.2022.1.0400]
36. Raschle S, Stephan R, Stevens MJ, Cernela N, Zurfluh K, Muchaamba F, et al. Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland. Sci Rep. 2021;11(1):9066. [DOI:10.1038/s41598-021-88514-y] [PMID] [PMCID]
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