year 1, Issue 2 (Summer 2007)                   Iran J Med Microbiol 2007, 1(2): 1-8 | Back to browse issues page

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Bakhtiari R, Soltan Dallal M, Zaemi Yazdi J, Fallah J, Amir Mozaffari N, Pourmand M et al . Evaluation of PCR method for diagnosis of Group B Streptococcuscarriage in pregnant women. Iran J Med Microbiol. 2007; 1 (2) :1-8
1- Science and Research Compose Islamic Azad University, Tehran
2- Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran
3- Department of Pathobiology, School of Medicine,Yazd Shaid Sadoughi, Yazd University of Medical Sciences, Yazd
4- Modern Age Bioinformatics Institute, Tehran
5- Department of Microbiology, School of Medicine, Iran University of Medical Sciences
Abstract:   (12439 Views)
Background and Objectives: Group B Streptococcus (GBS) (Streptococcus agalactiae) is the leading cause of morbidity and mortality of newborn infants and accounted as a leading factor causing septicemia after birth in mothers. Infections in infants are usually acquired by contact with the genital tract of the mothers during labor and delivery. In two last decades, significant progress toward detection, prevention and treatment of pregnant women carrying GBS has been achieved. A rapid screening test for GBS that could accurately identify pregnant women carrying the bacteria at the time of delivery would obviate the need for prenatal screening. The standard method for the diagnosis of GBS colonization consists of culturing vaginal and anal secretions in a selective broth medium which inhibits the growth of other microorganisms. Today, it is accepted that PCR has a high sensitivity and specifically in diagnosis. The goal of this study was to screen pregnant woman carrying GBS by PCR.
Material and Methods: Samples were taken from anal and vaginal mucus of 125 pregnant women who were at 28-38 weeks of ingestion by swab. Samples were tested by standard culture using Todd Hewitt Broth and Blood Agar and also by PCR using primers specific for cfbgene.
Results: Culture identified 10 (8%) women as carriage of GBSout of 125 women tested. On the other hand, the PCR assay could identify 12 (9/6%) women positive for GBS. In comparison to culture results, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 98%, and 83%, respectively. The time required for PCR assay and culture were 2h and 36h,respectively.
Conclusion: We found that GBS can be detected rapidly and reliably by a PCR assay using combined vaginal and anal secretions from pregnant women at the time of delivery. Also this study shows that the rate of incidence of GBS is high in Iranian pregnant women. We, therefore, recommend screening of pregnant women for detecting of GBS emphatically
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Type of Study: Brief report | Subject: Molecular Microbiology
Received: 2013/11/10 | Accepted: 2013/11/11

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