year 8, Issue 2 (8-2014)
Iran J Med Microbiol 2014, 8(2): 41-47 |
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Safarpordehkordi F, Yahaghi E, Khodaverdi Darian E. Prevalence of Antibiotic Resistance in Escherichia coli Isolated from Poultry Meat Supply in Isfahan. Iran J Med Microbiol 2014; 8 (2) :41-47
URL: http://ijmm.ir/article-1-187-en.html
URL: http://ijmm.ir/article-1-187-en.html
Prevalence of Antibiotic Resistance in Escherichia coli Isolated from Poultry Meat Supply in Isfahan
1- 1. Department of Food Hygiene, College of Veterinary Medicine, University of Tehran, Tehran, Iran , Dr.Farhads@yahoo.com
2- baqiyatallah universityn of medical sciences
3- young researchers and elite club karaj branch
2- baqiyatallah universityn of medical sciences
3- young researchers and elite club karaj branch
Abstract: (15875 Views)
Background and Aim: Unfortunately, conventional antibiotic therapies which were used for cases of food poisoning caused by Escherichia coli are costly and ineffective. This study was carried out in order to detection and investigates the antibiotic resistance pattern of Escherichia coli isolated from chicken meat distributed in Isfahan province.
Materials and Methods: 220 chicken meat samples were collected from shopping centers in Isfahan province. Samples were initially cultured and genomic DNA was extracted from the typical colonies that indicate the presence of Escherichia coli and polymerase chain reaction for diagnosis and detection of genes encoding resistance to antibiotics was used. Finally, the antibiotic resistance pattern was studied using the simple disk diffusion method.
Results: Totally, 20.45% of samples were contaminated with Escherichia coli. Bacterial isolates has the highest antibiotic resistance to gentamicin (84.44%), ampicilin (80%), ciprofloxacin (77.77%), enrofloxacin (66.66%) and erythromycin (22.62%). The genes encoding resistance against gentamicin (aac(3)-IV), sulfonamide (sul1) and ampicillin (CITM) with incidence rate of 88.88%, 86.66% and 84.44% had the highest frequencies, respectively.
Conclusions: Despite the high contamination rate of chicken meat with Escherichia coli, majority of isolates had high resistance to common antibiotics. Complete cooking of meat and avoid indiscriminate prescribing of antibiotics, preventing the occurrence of food poisoning due to resistant Escherichia coli.
Materials and Methods: 220 chicken meat samples were collected from shopping centers in Isfahan province. Samples were initially cultured and genomic DNA was extracted from the typical colonies that indicate the presence of Escherichia coli and polymerase chain reaction for diagnosis and detection of genes encoding resistance to antibiotics was used. Finally, the antibiotic resistance pattern was studied using the simple disk diffusion method.
Results: Totally, 20.45% of samples were contaminated with Escherichia coli. Bacterial isolates has the highest antibiotic resistance to gentamicin (84.44%), ampicilin (80%), ciprofloxacin (77.77%), enrofloxacin (66.66%) and erythromycin (22.62%). The genes encoding resistance against gentamicin (aac(3)-IV), sulfonamide (sul1) and ampicillin (CITM) with incidence rate of 88.88%, 86.66% and 84.44% had the highest frequencies, respectively.
Conclusions: Despite the high contamination rate of chicken meat with Escherichia coli, majority of isolates had high resistance to common antibiotics. Complete cooking of meat and avoid indiscriminate prescribing of antibiotics, preventing the occurrence of food poisoning due to resistant Escherichia coli.
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2013/12/12 | Accepted: 2014/05/12 | ePublished: 2014/05/28
Received: 2013/12/12 | Accepted: 2014/05/12 | ePublished: 2014/05/28
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