year 1, Issue 4 (Winter 2008)
Iran J Med Microbiol 2008, 1(4): 13-20 |
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Shoja S, Nahaei M, Farajnia S, Ahangarzadeh Rezaee M, Nikvash S. Comparison of Oxacillin Agar Screening and PCR Methods in Detection of Methicillin Resistance Staphylococcus epidermidis Strains Isolated from Blood Cultures of Children . Iran J Med Microbiol 2008; 1 (4) :13-20
URL: http://ijmm.ir/article-1-97-en.html
URL: http://ijmm.ir/article-1-97-en.html
Said Shoja1 
, Mohammadreza Nahaei 2, SafarAli Farajnia3 , Mohhamad Ahangarzadeh Rezaee1 , Solmaz Nikvash4
, Mohammadreza Nahaei 2, SafarAli Farajnia3 , Mohhamad Ahangarzadeh Rezaee1 , Solmaz Nikvash4
1- Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz
2- Drug Applied Research Center, TabrizUniversity of Medical Sciences, Tabriz , nahaeimr@yahoo.com
3- Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz
4- Doctor of laboratory Sciences, Tabriz University of Medical Sciences
2- Drug Applied Research Center, TabrizUniversity of Medical Sciences, Tabriz , nahaeimr@yahoo.com
3- Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz
4- Doctor of laboratory Sciences, Tabriz University of Medical Sciences
Abstract: (16696 Views)
Background and Objectives: In recent years methicillin resistant Staphylococcus epidermidis is among the
prevalent bacteria causing septicemia in neonates and children. Therefore the rapid diagnosis and their
antibiotic resistance surveillance are of great importance for treatment. The aim of this study was to evaluate
oxacillin screening agar with three concentrations of oxacillin and to compare with PCR in detection of
methicillin resistant Staphyliococcus epidermidis.
Material and Methods: A total of 100 Staphylococcus epidermidis strains were recovered from blood cultures of neonates and children. Resistance of isolates to methicillin were checked using oxacillin agar screening containing 0.6, 4, and 6 µg/ml of oxacillin. They were also tested formecA gene by PCR.
Results: According to PCR results 89 of test isolates revealed to have mecA gene. Oxacillin agar screening test containing 0.6 µg/ml oxacillin could detect 85 and 89 resistant strains after 24h and 48h incubation, respectively. The same test containing 4µg/ml oxacillin could detect 78 and 89 resistant strains after 24h and 48h incubation, respectively and by using 6 µg/ml oxacillin 67 and 75 resistant strains detected after 24h and 48h incubation, respectively.
Conclusion: Sensitivity of oxacillin agar screening test containing oxacillin concentrations of 0.6µg/ml and 4µg/ml after 48h incubation were the same as PCR test, whereas 6µg/ml oxacillin showed low sensitivity in detection of resistant isolates. Therefore, oxacillin agar screening test with 0.6 µg/ml and 4 µg/ml after 48h incubation, which is a cheap and handy for any level of routine laboratory could be used in accurate and effective detection of methicillin resistant S. epidermidis(MRSE).
Material and Methods: A total of 100 Staphylococcus epidermidis strains were recovered from blood cultures of neonates and children. Resistance of isolates to methicillin were checked using oxacillin agar screening containing 0.6, 4, and 6 µg/ml of oxacillin. They were also tested formecA gene by PCR.
Results: According to PCR results 89 of test isolates revealed to have mecA gene. Oxacillin agar screening test containing 0.6 µg/ml oxacillin could detect 85 and 89 resistant strains after 24h and 48h incubation, respectively. The same test containing 4µg/ml oxacillin could detect 78 and 89 resistant strains after 24h and 48h incubation, respectively and by using 6 µg/ml oxacillin 67 and 75 resistant strains detected after 24h and 48h incubation, respectively.
Conclusion: Sensitivity of oxacillin agar screening test containing oxacillin concentrations of 0.6µg/ml and 4µg/ml after 48h incubation were the same as PCR test, whereas 6µg/ml oxacillin showed low sensitivity in detection of resistant isolates. Therefore, oxacillin agar screening test with 0.6 µg/ml and 4 µg/ml after 48h incubation, which is a cheap and handy for any level of routine laboratory could be used in accurate and effective detection of methicillin resistant S. epidermidis(MRSE).
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2013/11/14 | Accepted: 2013/11/14 | ePublished: 2013/11/14
Received: 2013/11/14 | Accepted: 2013/11/14 | ePublished: 2013/11/14
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