The Effect of Matricaria chamomilla Alcoholic Extract on Phenotype Detection of Efflux Pumps of Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin lesions

Abdi, Pargol; Mahdavi Ourtakand, Maasoumeh; Honarmand Jahromy, Sahar

doi:10.30699/ijmm.13.3.220

year 13, Issue 3 (July - August 2019) Iran J Med Microbiol 2019, 13(3): 220-231 | Back to browse issues page

‎ 10.30699/ijmm.13.3.220

Mendeley

Zotero

RefWorks

Abdi P, Mahdavi Ourtakand M, Honarmand Jahromy S. The Effect of Matricaria chamomilla Alcoholic Extract on Phenotype Detection of Efflux Pumps of Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin lesions. Iran J Med Microbiol 2019; 13 (3) :220-231
URL: http://ijmm.ir/article-1-950-en.html

The Effect of Matricaria chamomilla Alcoholic Extract on Phenotype Detection of Efflux Pumps of Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin lesions

Pargol Abdi¹

, Maasoumeh Mahdavi Ourtakand

², Sahar Honarmand Jahromy¹

1- Department of Microbiology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran
2- Islamic Azad University , masumehmahdavi@gmail.com

Abstract: (4994 Views)

Background and Aims: Methicillin-resistant Staphylococcus aureus strains are resistant to many antibiotics. One of the major causes of drug resistance in bacteria is the efflux pumps. Matricaria chamomilla is a plant that is effective in treating skin diseases. The aim of this study was to investigate the effect of M. chamomilla alcoholic extract on phenotype detection and inhibition of efflux pumps of methicillin resistant S. aureus (MRSA) isolated from skin lesions.

Materials and Methods: The study was performed on 30 strains of S. aureus isolates collected from skin infections. The Strains of MRSA were detected by cefoxitin disc diffusion test. M. chamomilla alcoholic extract was prepared and its MIC was obtained against MRSA strains by broth microdilution method. Cartwheel method was used to study phenotypic activity of efflux pump. The effect of M. chamomilla extract was investigated on strains with active efflux pumps.

Results and Conclusion: from 30 strains of isolated S. aureus, 33.3% were methicillin-resistant. Three strains (10%) had active efflux pump, among which one strain was detected as MRSA. MIC of M. chamomilla extract was between 12-64 μg/ml. With the effect of ½ MIC of M. chamomilla extract on the strains with a strong efflux pumps, the two stains were reported as non-actives. M. chamomilla extract on the MRSA strain efflux pump was ineffective. Antimicrobial effect of M. chamomilla extract was confirmed on MRSA isolated from skin lesions.

Keywords: Matricaria chamomilla, Phenotype, MRSA, Antimicrobial Drug Resistance, Antimicrobial Agents.

Full-Text [PDF 997 kb] (2154 Downloads) | | Full-Text (HTML) (1675 Views)

Type of Study: Brief Original Article | Subject: Nosocomial infections
Received: 2019/07/29 | Accepted: 2019/11/22 | ePublished: 2019/11/22

References

1. Imanifooladi AA, Sattari M, Peerayeh SN, Hassan ZM, Hossainidoust SR. Detection the Staphylococcus aureus producing enterotoxin isolated from skin infections in hospitalized patients. Pakistan journal of biological sciences. PJBS 2007; 10(3):502-5. [DOI:10.3923/pjbs.2007.502.505] [PMID]

2. Shahidi-Dadras M, Toossi P, Sarrafi-Rad N, Robati RM, Saeedi M, Kavand S. Staphylococcus aureus carriage in patients with psoriasis. Iranian Journal of Dermatology. 2009;12(1):1-3.

3. Jappe U. Superantigens and their association with dermatological inflammatory diseases: facts and hypotheses. Acta dermato-venereologica. 2000; 80(5):321-8. [DOI:10.1080/000155500459231] [PMID]

4. Tomi NS, Kränke B, Aberer E. Staphylococcal toxins in patients with psoriasis, atopic dermatitis, and erythroderma, and in healthy control subjects. Journal of the American Academy of Dermatology. 2005; 1;53(1):67-72. [DOI:10.1016/j.jaad.2005.02.034] [PMID]

5. Webber MA, Piddock LJ. The importance of efflux pumps in bacterial antibiotic resistance. Journal of Antimicrobial Chemotherapy. 2003; 1;51(1):9-11. [DOI:10.1093/jac/dkg050] [PMID]

6. Blanco P, Hernando-Amado S, Reales-Calderon J, Corona F, Lira F, Alcalde-Rico M, Bernardini A, Sanchez M, Martinez J. Bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. Microorganisms. 2016 4(1): [DOI:10.3390/microorganisms4010014] [PMID] [PMCID]

7. Gibbons S. Phytochemicals for bacterial resistance-strengths, weaknesses and opportunities. Planta medica. 2008; 74(06):594-602. [DOI:10.1055/s-2008-1074518] [PMID]

8. Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from natural sources. Journal of antimicrobial chemotherapy. 2006; 4;59(6):1247-60. [DOI:10.1093/jac/dkl460] [PMID]

9. Gibbons S, Oluwatuyi M, Kaatz GW. A novel inhibitor of multidrug efflux pumps in Staphylococcus aureus. Journal of Antimicrobial Chemotherapy. 2003; 1;51(1):13-7. [DOI:10.1093/jac/dkg044] [PMID]

10. Tegos G, Stermitz FR, Lomovskaya O, Lewis K. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrobial agents and chemotherapy. 2002; 1;46(10):3133-41. [DOI:10.1128/AAC.46.10.3133-3141.2002] [PMID] [PMCID]

11. Pumps E, Cost F, RamA B. Efflux pumps of gram-negative bacteria: genetic responses to stress and the modulation of their activity by pH, inhibitors, and phenothiazines. Advances in enzymology and related areas of molecular biology. 2011; 15;238:61. [DOI:10.1002/9780470920541.ch2]

12. Amaral L, Cerca P, Spengler G, Machado L, Martins A, Couto I, et al. Ethidium bromide efflux by Salmonella: modulation by metabolic energy, pH, ions and phenothiazines. International journal of antimicrobial agents. 2011; 38(2): 140-5. [DOI:10.1016/j.ijantimicag.2011.03.014] [PMID]

13. Abdoul-Latif FM, Mohamed N, Edou P, Ali AA, Djama SO, Obame LC, Bassolé IH, Dicko MH. Antimicrobial and antioxidant activities of essential oil and methanol extract of Matricaria chamomilla L. from Djibouti. Journal of Medicinal Plants Research. 2011; 4;5(9):1512-7.

14. Charousaei F, Dabirian A, Mojab F. Using chamomile solution or a 1% topical hydrocortisone ointment in the management of peristomal skin lesions in colostomy patients: results of a controlled clinical study. Ostomy-Wound Management. 2011; 1;57(5):28.

15. Sharifi A, Mohammadzadeh A, Mahmoodi P, Sasanian N. Inhibitory Effect of Thymus daenensis Essential Oil on Staphylococcus aureus NorA Efflux Pump. J Adv Med Biomed Res. 2016; 24 (105) :67-77

16. Martins M, McCusker MP, Viveiros M, Couto I, Fanning S, Pagès JM, Amaral L. A simple method for assessment of MDR bacteria for over-expressed efflux pumps. The open microbiology journal. 2013; 7:72. [DOI:10.2174/1874285801307010072] [PMID] [PMCID]

17. Movagharnezhad M, khataminezhad M R. Identification and Characterization of Staphylococcus Aureus Methicillin and Vancomycin Resistance From Patients in Sari and Ghaemshahr Injuries and Burn Hospitals in 2015. Iran J Med Microbiol. 2018; 12 (3) :160-168 [DOI:10.30699/ijmm.12.3.160]

18. Fagheei-Aghmiyuni Z, Khorshidi A, Soori T, Moniri R, Mousavi S G A. Antibiotic susceptibility pattern and the prevalence of Staphylococcus aureus isolated from skin and soft tissue in Tehran Razi skin hospital (2014-15). Feyz. 2017; 21 (2) :188-196

19. Motamedi H, Rahmat Abadi SS, Moosavian SM, Torabi M. The Association of PantonValentine leukocidin and mecA Genes in Methicillin-Resistant Staphylococcus aureus Isolates From Patients Referred to Educational Hospitals in Ahvaz, Iran. Jundishapur J Microbiol 2015; 8(8). [DOI:10.5812/jjm.22021v2] [PMID] [PMCID]

20. Jarrahi M, Vafaei AA, Taherian AA, Miladi H, Rashidi Pour A. Evaluation of topical Matricaria chamomilla extract activity on linear incisional wound healing in albino rats. Natural product research. 2010; 10;24(8):697-702. [DOI:10.1080/14786410701654875] [PMID]

21. Roby MH, Sarhan MA, Selim KA, Khalel KI. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L.). Industrial crops and products. 2013; 1(44):437-45. [DOI:10.1016/j.indcrop.2012.10.012]

22. Piddock LJ, Garvey MI, Rahman MM, Gibbons S. Natural and synthetic compounds such as trimethoprim behave as inhibitors of efflux in Gram-negative bacteria. Journal of Antimicrobial Chemotherapy. 2010; 19; 65(6):1215-23. [DOI:10.1093/jac/dkq079] [PMID]

23. Haddadi Zahmatkesh M A, Laripoor M, Mirzaie A, Ashrafi F. Prevalence of norA and norB efflux pump genes in clinical isolates of Staphylococcus aureus and their contribution in ciprofloxacin resistance. Iran J Med Microbiol. 2016; 10 (5) :20-30

24. Martins M, Viveiros M, Couto I, Costa SS, Pacheco T, Fanning S, Pages JM, Amaral L. Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method. in vivo. 2011; 1;25(2):171-8.

25. Patel D, Kosmidis C, Seo SM, Kaatz GW. Ethidium bromide MIC screening for enhanced efflux pump gene expression or efflux activity in Staphylococcus aureus. Antimicrobial agents and chemotherapy. 2010; 1;54(12):5070-3. [DOI:10.1128/AAC.01058-10] [PMID] [PMCID]

26. Smith EC, Kaatz GW, Seo SM, Wareham N, Williamson EM, Gibbons S. The phenolic diterpene totarol inhibits multidrug efflux pump activity in Staphylococcus aureus. Antimicrobial agents and chemotherapy. 2007; 1;51(12):4480-3. [DOI:10.1128/AAC.00216-07] [PMID] [PMCID]

27. Sana M, Jameel H, Rahman M. Miracle remedy: Inhibition of bacterial efflux pumps by natural products. Journal of Infectious Diseases & Therapy. 2015; 30. [DOI:10.4172/2332-0877.1000213]

28. Khan IA, Mirza ZM, Kumar A, Verma V, Qazi GN. Piperine, a phytochemical potentiator of ciprofloxacin against Staphylococcus aureus. Antimicrobial agents and chemotherapy. 2006; 1;50(2):810-2. [DOI:10.1128/AAC.50.2.810-812.2006] [PMID] [PMCID]