year 14, Issue 4 (July - August 2020)
Iran J Med Microbiol 2020, 14(4): 379-387 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Dehghan D, Fasihi-Ramandi M, Taheri R. Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion. Iran J Med Microbiol 2020; 14 (4) :379-387
URL: http://ijmm.ir/article-1-947-en.html
URL: http://ijmm.ir/article-1-947-en.html
1- Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Molecular Biology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran ,r.a.taheri@gmail.com
2- Molecular Biology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran ,
Abstract: (3890 Views)
Background: Pseudomonas aeruginosa infections are resistant to antimicrobial agents and produce toxic virulence factors such as exotoxin A. Studies have shown that some nanoparticle compounds and antibiotics have a synergistic effect. Therefore, the aim of this study was to investigate the synergistic effect of silver nanoparticles and erythromycin on antibiotic-resistant P. aeruginosa.
Materials & Methods: In this descriptive cross-sectional study, 40 cultured samples of burn wound secretions were taken from Imam Musa Kazem (PBUH) Burns Hospital in Isfahan, Iran. Diagnostic and differential tests were performed. Antibiogram was performed to obtain the bacterial resistance pattern and the exotoxin A gene was detected by PCR. The bacterial minimum inhibitory concentration (MIC) was then applied to the silver nanoparticles (shape and mean size) and erythromycin separately and a common mixture of both in 10 dilutions to investigate the synergistic effect.
Results & Conclusion: A number of 26 bacteria were strains of P. aeruginosa. Of samples, 25 (96.15%) had exotoxin A gene. All samples were sensitive to all erythromycin concentrations. The mean MIC of nanoparticles against bacteria was reported to be 2 μg/mL. A solution of 40 μg/mL erythromycin and 2 μg/mL nanoparticles was also considered as MIC solution. Pseudomonas aeruginosa is sensitive to erythromycin to very low concentrations of silver particles. But no synergistic effect between silver nanoparticles and erythromycin was reported for this bacterium. Based on PCR results and antibiotic resistance pattern, a significant number of the samples contained the exotoxin A gene and the use of erythromycin alone was not appropriate for treatment.
Materials & Methods: In this descriptive cross-sectional study, 40 cultured samples of burn wound secretions were taken from Imam Musa Kazem (PBUH) Burns Hospital in Isfahan, Iran. Diagnostic and differential tests were performed. Antibiogram was performed to obtain the bacterial resistance pattern and the exotoxin A gene was detected by PCR. The bacterial minimum inhibitory concentration (MIC) was then applied to the silver nanoparticles (shape and mean size) and erythromycin separately and a common mixture of both in 10 dilutions to investigate the synergistic effect.
Results & Conclusion: A number of 26 bacteria were strains of P. aeruginosa. Of samples, 25 (96.15%) had exotoxin A gene. All samples were sensitive to all erythromycin concentrations. The mean MIC of nanoparticles against bacteria was reported to be 2 μg/mL. A solution of 40 μg/mL erythromycin and 2 μg/mL nanoparticles was also considered as MIC solution. Pseudomonas aeruginosa is sensitive to erythromycin to very low concentrations of silver particles. But no synergistic effect between silver nanoparticles and erythromycin was reported for this bacterium. Based on PCR results and antibiotic resistance pattern, a significant number of the samples contained the exotoxin A gene and the use of erythromycin alone was not appropriate for treatment.
Keywords: Silver nanoparticles, Pseudomonas aeruginosa, Erythromycin, Minimum Concentration, Exotoxin A
Type of Study: Brief Original Article |
Subject:
Nanotechnology In Medicine
Received: 2019/07/7 | Accepted: 2020/07/9 | ePublished: 2020/07/9
Received: 2019/07/7 | Accepted: 2020/07/9 | ePublished: 2020/07/9
References
1. Sharma BK, Saha A, Rahaman L, Bhattacharjee S, Tribedi P. Silver inhibits the biofilm formation of Pseudomonas aeruginosa. Adv Microbiol. 2015 Sep 7;5(10):677. [DOI:10.4236/aim.2015.510070]
2. Natsuki J, Natsuki T, Hashimoto Y. A review of silver nanoparticles: synthesis methods, properties and applications. Int. J. Mater. Sci. Appl. 2015;4(5):325-32. [DOI:10.11648/j.ijmsa.20150405.17]
3. Lee VT, Smith RS, Tümmler B, Lory S. Activities of Pseudomonas aeruginosa effectors secreted by the Type III secretion system in vitro and during infection. Infect Immun. 2005 Mar 1;73(3):1695-705. [DOI:10.1128/IAI.73.3.1695-1705.2005] [PMID] [PMCID]
4. Kaszab E, Szoboszlay S, Dobolyi C, Háhn J, Pék N, Kriszt B. Antibiotic resistance profiles and virulence markers of Pseudomonas aeruginosa strains isolated from composts. Bioresour Technol. 2011 Jan 1;102(2):1543-8. [DOI:10.1016/j.biortech.2010.08.027] [PMID]
5. Seshadri S, Prakash A, Kowshik M. Biosynthesis of silver nanoparticles by marine bacterium, Idiomarina sp. PR58-8. Bull Mater Sci. 2012 Dec 1;35(7):1201-5. [DOI:10.1007/s12034-012-0417-0]
6. Shinashal RZ. The effect of Silver nanoparticles in the treatment of Pseudomonas aeruginosa infections. J Pharmaceut Sci Res. 2019;11(1):58-60.
7. Ahmadi M, Adibhesami M. The effect of silver nanoparticles on wounds contaminated with pseudomonas aeruginosa in mice: An experimental study.IJPR. 2017;16(2):661.
8. Nasiri A, Gharebagh RA, Nojoumi SA, Akbarizadeh M, Harirchi S, Arefnezhad M .et al. Evaluation of the antimicrobial activity of silver nanoparticles on antibiotic-resistant Pseudomonas aeruginosa. IJBMS. 2016 Jun 29;1(1):25-8. [DOI:10.15171/ijbsm.2016.06]
9. Yousefi-Avarvand A, Khashei R, Ebrahim-Saraie HS, Emami A, Zomorodian K, Motamedifar M. The frequency of exotoxin A and exoenzymes S and U genes among clinical isolates of Pseudomonas aeruginosa in Shiraz, IJMCM. 2015;4(3):167.
10. Salas-Orozco M, Niño-Martínez N, Martínez-Castañón GA, Méndez FT, Jasso ME, Ruiz F. Mechanisms of Resistance to Silver Nanoparticles in Endodontic Bacteria: A Literature Review. J Nanomater. 2019;2019. [DOI:10.1155/2019/7630316]
11. Habash MB, Park AJ, Vis EC, Harris RJ, Khursigara CM. Synergy of silver nanoparticles and aztreonam against Pseudomonas aeruginosa PAO1 biofilms. Antimicrob Agents Chemother. 2014 Oct 1;58(10):5818-30. [DOI:10.1128/AAC.03170-14] [PMID] [PMCID]
12. He T, Liu H, Zhou Y, Yang J, Cheng X, Shi H. Antibacterial effect and proteomic analysis of graphene-based silver nanoparticles on a pathogenic bacterium Pseudomonas aeruginosa. Biometals. 2014 Aug 1;27(4):673-82. [DOI:10.1007/s10534-014-9756-1] [PMID]
13. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004 Jul 1;275(1):177-82. [DOI:10.1016/j.jcis.2004.02.012] [PMID]
14. Kalishwaralal K, BarathManiKanth S, Pandian SR, Deepak V, Gurunathan S. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B Biointerfaces. 2010 Sep 1;79(2):340-4. [DOI:10.1016/j.colsurfb.2010.04.014] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
