Assessment of Lactobacillus Delbruekii and Bifidobacterium Animalis Abilities to Absorb Aflatoxin M1 from Milk

Namvarrad, Maryam; Razavilar, Vadood; Anvar, Seyed Amir Ali; Akbari-Adergani, Behrouz

doi:10.30699/ijmm.13.1.44

year 13, Issue 1 (March - April 2019) Iran J Med Microbiol 2019, 13(1): 44-55 | Back to browse issues page

‎ 10.30699/ijmm.13.1.44

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Namvarrad M, Razavilar V, Anvar S A A, Akbari-Adergani B. Assessment of Lactobacillus Delbruekii and Bifidobacterium Animalis Abilities to Absorb Aflatoxin M1 from Milk. Iran J Med Microbiol 2019; 13 (1) :44-55
URL: http://ijmm.ir/article-1-890-en.html

Assessment of Lactobacillus Delbruekii and Bifidobacterium Animalis Abilities to Absorb Aflatoxin M1 from Milk

Maryam Namvarrad¹

, Vadood Razavilar

², Seyed Amir Ali Anvar¹

, Behrouz Akbari-Adergani³

1- Department of Food Hygiene, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Food Hygiene, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. , vazneh2245@gmail.com
3- Food and Drug Laboratory Research Center, Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.

Abstract: (5543 Views)

Background and Aims: Microbial detoxification is one of the methods for eliminating of aflatoxins, including aflatoxin M₁. Reports indicate that some strains of lactic acid bacteria family through surface adsorption of aflatoxin in their cellwall can be effective in removing them and as a primer culture. In this study, the ability of Bifidobacterium animalis and Lactobacillus delbrueckii in the adsorption of aflatoxin M₁in skim milk was assessed.
Materials and Methods: For this purpose, about 10⁸ and 10⁹ cfu/ ml of B. animalis (Lactis) and L. delbrueckii (Blegaricus) were inoculated into skim milk without aflatoxin M₁. Then, the samples were spiked by aflatoxin M₁ in concentrations of 0.25, 0.5 and 0.75 ng/ ml. The concentration of the aflatoxin reside in supernatant of milk samples after different storage times (0.5, 1, 2 and 24 h) and temperatures of 4 and 37°C was measured by ELISA method, and the results were confirmed by HPLC.
Results: The results showed that the highest amount of aflatoxin M₁ removal was respectively related to B. animalis (60 ± 2.5%) with a concentration of 10⁸ cells/ ml and L. delbrueckii (58.5 ± 2.5%) with a concentration of 10⁹ cells/ ml and a concentration of 0.5 ng/ml poison at 37°C for 30 minutes. By comparing the concentration of both bacteria, it also appeared that the B. animalis concentration at 37°C and L. delbrueckii concentration at 4°C were more effective. Also, the results indicate that the ability of bacteria to reduce the amount of poison in half an hour in milk samples with values of 0.75 ng/ml poison at 4°C and 0.5 ng/ml poison at 37°C is higher; but over time, contaminated milk at a concentration of 0.75 ng/ml poison compared to 0.5 ng/ml poison showed an increased amount of aflatoxin removal.
Calclusion: B. animalis and L. delbrueckii can act as two useful probiotics to reduce the harmful effects of aflatoxin M₁.

Keywords: Aflatoxin M1, Probiotic, Detoxification, B. animalis, L. delberueckii, Skim milk.

Full-Text [PDF 1569 kb] (2174 Downloads)

Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2018/11/16 | Accepted: 2019/04/10 | ePublished: 2019/07/22

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