year 13, Issue 2 (May - June 2019)
Iran J Med Microbiol 2019, 13(2): 89-101 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Asadi N, Hazrati Tappeh K, Yousefi E, Khademvatan S. Differentiation of prevalent parasite from artifacts in parasitology laboratory. Iran J Med Microbiol 2019; 13 (2) :89-101
URL: http://ijmm.ir/article-1-842-en.html
URL: http://ijmm.ir/article-1-842-en.html
1- Department of Parasitology and Mycology, Center for Cellular and Molecular Research, Faculty of Medicine, Urmia University of Medical Sciences, Iran
2- Department of Parasitology and Mycology, School of public health, Tehran University of Medical Sciences, Iran
3- Department of Parasitology and Mycology, Center for Cellular and Molecular Research, Faculty of Medicine, Urmia University of Medical Sciences, Iran , khademvatan@yahoo.com
2- Department of Parasitology and Mycology, School of public health, Tehran University of Medical Sciences, Iran
3- Department of Parasitology and Mycology, Center for Cellular and Molecular Research, Faculty of Medicine, Urmia University of Medical Sciences, Iran , khademvatan@yahoo.com
Abstract: (10771 Views)
One of the common problems in the medical parasitology laboratory is the differentiation of parasites from other elements in the stool and body fluids so-called "artifacts". Artifacts generally referred to living or abiotic agents that embedded in the clinical sample and may misleading the lab because of their similarity to parasitic organisms. Artifacts are an integral part of the diagnosis process and they are cause of common misdiagnosis in the laboratory.
Their differentiation from pathogenic parasitic agents is done by proper diagnosis, which leads to proper treatment of parasitic infections. As usually an inexperienced technician often misdiagnosed a yeast or other plant cell as amoeba or considers a platelet as a malaria parasite, it may be unsuccessful to identify parasitic organisms that really exist in the stool sample.
The major forms that cause confusion and misdiagnosis in parasitology laboratory are spores, fat droplets, yeast, red blood cells, and macrophages.Compared with other parts of the medical laboratory, in parasitology lab less attention observe to this problem. The consequence is the reporting of false positive results, incorrect treatment, and patient injury.
Identifying, introducing, and differentiating artifacts for laboratory personnel, especially inexperienced, are an important factor in accurate diagnosis. In the present study, key diagnostic points of parasitic organisms and artifacts have been categories.
Their differentiation from pathogenic parasitic agents is done by proper diagnosis, which leads to proper treatment of parasitic infections. As usually an inexperienced technician often misdiagnosed a yeast or other plant cell as amoeba or considers a platelet as a malaria parasite, it may be unsuccessful to identify parasitic organisms that really exist in the stool sample.
The major forms that cause confusion and misdiagnosis in parasitology laboratory are spores, fat droplets, yeast, red blood cells, and macrophages.Compared with other parts of the medical laboratory, in parasitology lab less attention observe to this problem. The consequence is the reporting of false positive results, incorrect treatment, and patient injury.
Identifying, introducing, and differentiating artifacts for laboratory personnel, especially inexperienced, are an important factor in accurate diagnosis. In the present study, key diagnostic points of parasitic organisms and artifacts have been categories.
Type of Study: Review Article |
Subject:
Medical Parasitology
Received: 2018/06/11 | Accepted: 2019/08/14 | ePublished: 2019/09/15
Received: 2018/06/11 | Accepted: 2019/08/14 | ePublished: 2019/09/15
References
1. Arbabi M, Talari S. Intestinal parasites in student of Kashan university of medical sciences. Journal of Ilam University of Medical Sciences. 2004:44-5.
2. Bundy D, Hall A, Medley G, Savioli L. Evaluating measures to control intestinal parasitic infections. 1992.
3. Musaiger A. Intestinal parasitic infections among school children in Bahrain. Journal of tropical pediatrics. 1989;35(1):45-6. [DOI:10.1093/tropej/35.1.45] [PMID]
4. Heitman J. Mandell, Douglas, and Bennett's principles and practice of infectious diseases. Mycopathologia. 2000 Aug 25;149(1):47-8.
5. Montresor A, Crompton DW, Hall A, Bundy D, Savioli L, Organization WH. Guidelines for the evaluation of soil-transmitted helminthiasis and schistosomiasis at community level: a guide for managers of control programmes. 1998.
6. MoghateliM, Gorgipur M, Mohamadzade M, Rahimi Pordanjani S, Namrudi J, Soleymani M. Frequency of intestinal parasitic infections in the Dashti county (Bushehr province) during 2009 to 2014. Navid No. 2015;17(59):15-21.
7. Garcia LS AL. Diagnostic Parasitology. ed2.1979.
8. Smith JW MR, Ash LR, Melvin DM, Orihel TC, Thompson JH. Diagnostic Medical Parasitology: Intestinal Protozoa 1976.
9. http://www.provlab.ab.ca/webbug/parasite/artifact/intro.htm.
10. Parija S, Bhattacharya S, Padhan P, Shivaprakash M. Evaluation of Formalin-Acetone Sedimentation in the Concentration of Stool for Intestinal Parasites. Tropical doctor. 2003;33(3):163-4. [DOI:10.1177/004947550303300315] [PMID]
11. Truant AL, Elliott SH, Kelly MT, Smith JH. Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques for detection of intestinal parasites. Journal of clinical microbiology. 1981;13(5):882-4.
12. Ramsay A, Gillespie S, Mnzava T, Ngowi F, Fox R. A field evaluation of the formol-detergent method for concentrating faecal parasites. The Journal of tropical medicine and hygiene. 1991;94(3):210-3.
13. Neimeister R, Logan AL, Gerber B, Egleton J, Kleger B. Hemo-De as substitute for ethyl acetate in formalin-ethyl acetate concentration technique. Journal of clinicalmicrobiology. 1987;25(2):425-6.
14. Young KH, Bullock SL, Melvin DM, Spruill CL. Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. Journal of clinical microbiology. 1979;10(6):852-3.
15. Fritsche TR, Selvarangan R. Medical parasitology. Henry's Clinical Diagnosis and Management by Laboratory Methods 22nd ed Philadelphia, Pa: Saunders Elsevier. 2011. [DOI:10.1016/B978-1-4377-0974-2.00062-2]
16. Madico G, Quinn TC, Rompalo A, McKee KT, Gaydos CA. Diagnosis of Trichomonas vaginalisInfection by PCR UsingVaginal Swab Samples. Journal of clinical microbiology. 1998;36(11):3205-10.
17. Draper D, Parker R, Patterson E, Jones W, Beutz M, French J, et al. Detection of Trichomonas vaginalis in pregnant women with the InPouch TV culture system. Journal of clinical microbiology. 1993;31(4):1016-8.
18. de Hoog GS CG, Figueras MJ. Atlas ofclinical fungi. 2001.
19. Winn W AS JW, Koneman E,, Procop G SP,woods, allen. . Koneman's color atlas and textbook of diagnostic2006.
20. Pherson RA PM. Henry's clinicaldiagnosis and management by laboratorymethods. 2011.
21. Gourguechon S, Holt LJ, Cande WZ. The Giardia cell cycle progresses independently of the anaphase-promoting complex. J Cell Sci. 2013;126(10):2246-55. [DOI:10.1242/jcs.121632] [PMID] [PMCID]
22. www.cdc.gov/parasites/.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
