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Iran J Med Microbiol 2018, 12(3): 0-0 Back to browse issues page
The Effect of Oncolytic Reovirus Infection on Nitric Oxide Secretion and Induction of Apoptosis in Adipose Tissue-Derived Mesenchymal Stem Cells
Razieh Sadat Banijamali 1, Hoorieh Soleimanjahi 2, Sara Soudi 3, Hesam Karimi 1
1- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , SOLEIM_H@modares.ac.ir
3- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (449 Views)
Background and Aims: Oncolytic viruses (OVs) are a new approach in treatment of cancer. Antitumor efficacy of OVs were limited due to insufficient and non-specific viral delivery to tumor sites. To overcome this issue, mesenchymal stem cells (MSCs) was used for its ability to specifically homing into tumors. The main aim of this study was to use MSCs as a carrier and investigate the effect of oncolytic reovirus infection in MSCs, induction of apoptosis, nitric oxide (NO) secretion and their effects for selectively killing tumor cells, to use in future.
Materials and Methods: MSCs isolated from mice adipose tissue and confirmed. Then, the ability of the virus to infect MSCs and the effect of reovirus infection in induction of apoptosis and NO secretion in MSCs were evaluated. 
Results: The results demonstrate that reovirus could replicate on MSCs. The finding indicated that the NO production significantly was higher at 72 h post infection with different MOI in comparison to the control cells. Also, reovirus induced high level of apoptosis in the MSCs at 48 h post infection compared with the control cells.
Conclusion: Based on observed results, reovirus increased the secretion of iNOS (inducible nitric oxide) in the infected MSCs at 48 h post infection; therefore, high amounts of NO and reovirus replication were found to trigger apoptosis at 48 h post infection. Therefore, by optimizing the replication time of virus in the MSCs, specific viral delivery to tumor sites are available and causes of cancer cells death.
Keywords: Oncolytic Reovirus T3D, Mesenchymal Stem Cell (MSC), Carrier Cell, Nitric Oxide (NO), Apoptosis, Cancer Therapy
     
Type of Study: Original | Subject: Medical Virology
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Banijamali R S, Soleimanjahi H, Soudi S, Karimi H. The Effect of Oncolytic Reovirus Infection on Nitric Oxide Secretion and Induction of Apoptosis in Adipose Tissue-Derived Mesenchymal Stem Cells . Iran J Med Microbiol. 2018; 12 (3)
URL: http://ijmm.ir/article-1-838-en.html


year 12, Issue 3 (July - August 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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