Prevalence of Mycoplasma pneumonia in Patients with Respiratory Infections from  Shaheed Mostafa Khomeini and Khatam Hospitals by Culture and PCR Methods

Pouladi, Iman; Taheri, Mohsen; Niakan, Mohammad; Mirnejad, Reza; Azimi, Gasem

doi:10.30699/ijmm.12.6.382

year 12, Issue 6 (January - February 2019) Iran J Med Microbiol 2019, 12(6): 382-389 | Back to browse issues page

‎ 10.30699/ijmm.12.6.382

Mendeley

Zotero

RefWorks

Pouladi I, Taheri M, Niakan M, Mirnejad R, Azimi G. Prevalence of Mycoplasma pneumonia in Patients with Respiratory Infections from Shaheed Mostafa Khomeini and Khatam Hospitals by Culture and PCR Methods . Iran J Med Microbiol 2019; 12 (6) :382-389
URL: http://ijmm.ir/article-1-837-en.html

Prevalence of Mycoplasma pneumonia in Patients with Respiratory Infections from Shaheed Mostafa Khomeini and Khatam Hospitals by Culture and PCR Methods

Iman Pouladi¹

, Mohsen Taheri¹

, Mohammad Niakan

², Reza Mirnejad³

, Gasem Azimi⁴

1- Department of Microbiology, Medicine Faculty, Shahed University, Tehran, Iran
2- Department of Microbiology, Medicine Faculty, Shahed University, Tehran, Iran , niakan@shahed.ac.ir
3- Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Departement of Internal Diseases, Shahid Mostafa Khomeni Hospital, Tehran, Iran

Abstract: (7996 Views)

Background and Aims: Mycoplasma pneumoniae is one of the most important pathogens causing human respiratory tract infection; especially in community-acquired pneumonia (responsible for 10 – 40% of these infections).The aim of this study was to evaluate the prevalence of Mycoplasma pneumonia in patients with respiratory infections from Mostafa Khomeini and Khatam hospitals, by culture and molecular methods.
Materials and Methods: In this study, 100 samples of throat swab from patients with respiratory infections were collected. All samples were cultured in PPLO broth and PPLO agar. After culture and genomic DNA extraction, PCR was carried out using specific M. pneumoniae primers.
Results: In this study, 14 (14%) colonies of Mycoplasma were isolated on PPLO agar medium. Using specific primers, 17 samples (17%) were detected as Mycoplasma genus and 6 samples (6%) were confirmed to be M. pneumoniae species.
Conclusions: Based on the results of this study, For the detection of M. pneumoniae among the respiratory infections cases PCR is a highly reliable and sensitive method compared to the culture media. Using specific primers, PCR can confidently detect and separate infectious agents even in the genesis and species level.

Keywords: Mycoplasma pneumonia, Polymerase chain reactions, Culture, Respiratory infections

Full-Text [PDF 901 kb] (1965 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2018/05/21 | Accepted: 2019/01/22 | ePublished: 2019/03/29

References

1. Amirian S, Amini K, Parviz M. Identification of Mycoplasma Pneumonia Isolated from the Respiratory Secree tions of Patients with Chronic Obstructive Pulmonary Disease Using Polymerase Chain Reaction in Kerman Province, Iran. Tabari J Prev Med. Winter 2016; 1(3): 8-15.

2. Gdalevich M, Haas EJ, Dukhan L, Katz M, Zelenski V, Moran-Gilad J. control of a Mycoplasma pneumoniae Outbreak in an institutional setting Using azithromycin Prophylaxis. Frontiers in public health. 2018; 5: 366. [DOI:10.3389/fpubh.2017.00366] [PMID] [PMCID]

3. Li W, Fang YH, Shen HQ, Yang DH, Shu Q, Shang SQ. Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia. PloS one. 2017; 12(5): e0177842. [DOI:10.1371/journal.pone.0177842] [PMID] [PMCID]

4. Wang L, Feng Z, Zhao M, Yang S, Yan X, Guo W, et al. A comparison study between GeXP-based multiplex-PCR and serology assay for Mycoplasma pneumoniae detection in children with community acquired pneumonia. BMC Infect Dis. 2017; 17(1): 518. [DOI:10.1186/s12879-017-2614-3] [PMID] [PMCID]

5. Topley Wilson,s Microbiology & Microbial infections, 10th Edition.

6. Kheiri B, Alhesan NA, Madala S, Assasa O, Shen M, Dawood T. Mycoplasma pneumoniae‐associated Fuchs syndrome. Clinical case reports. 2018; 6(2): 434. [DOI:10.1002/ccr3.1350] [PMID] [PMCID]

7. Yu Y, Fei A. Atypical pathogen infection in community-acquired pneumonia. Biosci Trends. 2016; 10(1): 7-13. [DOI:10.5582/bst.2016.01021] [PMID]

8. Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology-E-Book. Elsevier Health Sciences; 2014.

9. Sharma L, Losier A, Tolbert T, Cruz CS, Marion CR. Pneumonia updates on Legionella, Chlamydophila, and Mycoplasma pneumonia. Clinics in chest medicine. 2017; 38(1): 45. [DOI:10.1016/j.ccm.2016.11.011] [PMID] [PMCID]

10. Xu W, Guo L, Dong X, Li X, Zhou P, Ni Q, et al. Detection of Viruses and Mycoplasma pneumoniae in Hospitalized Patients with Severe Acute Respiratory Infection in Northern China, 2015-2016. Jpn J Infect Dis. 2018; 71(2): 134 - 9. [DOI:10.7883/yoken.JJID.2017.412] [PMID]

11. Bao YX, Li J, Tian Y, Liu QH, Bao J. Atopy: a risk factor of refractory mycoplasma pneumoniae pneumonia?. Clin Respir J. 2017; 11(6): 931-4. [DOI:10.1111/crj.12439] [PMID]

12. Wang ZH, Li XM, Wang YS, Guo ZY. Changes in the levels of interleukin-17 between atopic and non-atopic children with Mycoplasma pneumoniae pneumonia. Inflammation. 2016; 39(6): 1871-5. [DOI:10.1007/s10753-016-0422-3] [PMID]

13. Kim JH, Cho TS, Moon JH, Kim CR, Oh JW. Serial changes in serum eosinophil-associated mediators between atopic and non-atopic children after Mycoplasma pneumoniae pneumonia. Allergy Asthma Immunol Res. 2014; 6(5): 428-33. [DOI:10.4168/aair.2014.6.5.428] [PMID] [PMCID]

14. Dorairaj A, Kopula SS, Kumar K. Atypical Pneumonia-Screening in a Tertiary Care Centre. J Clin Diagn Res. 2015; 9(11): DC18.

15. Golmohammadi R, Ataee RA, Alishiri GH, Mirnejad R, Najafi A, Tate M, et al. Molecular diagnosis of Mycoplasma pneumoniae in synovial fluid of rheumatoid arthritis patients. Iran J Med Microbiol. 2014; 8(1): 1-8.

16. Page CA, Krause DC. Protein kinase/phosphatase function correlates with gliding motility in Mycoplasma pneumoniae. Journal of bacteriology. 2013; 195(8): 1750-7. [DOI:10.1128/JB.02277-12] [PMID] [PMCID]

17. Kawai Y, Miyashita N, Kubo M, Akaike H, Kato A, Nishizawa Y, et al. Nationwide surveillance of macrolide-resistant Mycoplasma pneumoniae infection in pediatric patients. Antimicrobial agents and chemotherapy. 2013; 57(8): 4046-9. [DOI:10.1128/AAC.00663-13] [PMID] [PMCID]

18. Tian XJ, Dong YQ, Dong XP, Li JY, Li D, Jiang Y, et al . P1 gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2010 and relationship between genotyping and macrolide resistance. Chinese Medical Journal. 2013; 126 (20): 3944-3947.

19. Cillóniz C, Torres A, Niederman M, van der Eerden M, Chalmers J, Welte T, Blasi F. Community-acquired pneumonia related to intracellular pathogens. Intensive care medicine. 2016; 42(9): 1374-86. [DOI:10.1007/s00134-016-4394-4] [PMID]

20. Spuesens EB, Meijer A, Bierschenk D, Hoogenboezem T, Donker GA, Hartwig NG, et al. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae in respiratory specimens collected between 1997 and 2008 in The Netherlands. Journal of clinical microbiology. 2012; 50(6): 1999-2004. [DOI:10.1128/JCM.00400-12] [PMID] [PMCID]

21. Meyer Sauteur PM, Unger WW, Nadal D, Berger C, Vink C, van Rossum A. Infection with and Carriage of Mycoplasma pneumoniae in Children. Front Microbiol. 2016; 7: 329. [DOI:10.3389/fmicb.2016.00329] [PMID] [PMCID]

22. Carrim M, Wolter N, Benitez AJ, Tempia S, du Plessis M, Walaza S, et al. Epidemiology and Molecular Identification and Characterization of Mycoplasma pneumoniae, South Africa, 2012-2015. Emerging infectious diseases. 2018; 24(3): 506. [DOI:10.3201/eid2403.162052] [PMID] [PMCID]

23. Chaudhry R, Valavane A, Sreenath K, Choudhary M, Sagar T, Shende T, et al. Detection of Mycoplasma pneumoniae and Legionella pneumophila in Patients Having Community-Acquired Pneumonia: A Multicentric Study from New Delhi, India. Am J Trop Med Hyg. 2017; 97(6): 1710-6. [DOI:10.4269/ajtmh.17-0249] [PMID] [PMCID]

24. Al-Ghizawi GJ, Al-Sulami AA, Al-Taher SS. Profile of community- and hospital-acquired pneumonia cases admitted to Basra General Hospital, Iraq. East Mediterr Health J. 2007; 13(2): 230-42.

25. Talkington DF, Thacker WL, Keller DW, Jensen JS. Diagnosis of Mycoplasma pneumoniaeInfection in Autopsy and Open-Lung Biopsy Tissues by Nested PCR. Journal of clinical microbiology. 1998; 36(4): 1151-3.

26. Zhang Y, Mei S, Zhou Y, Yang D, Pan T, Chen Z, Wang Q. TIPE2 negatively regulates mycoplasma pneumonia-triggered immune response via MAPK signaling pathway. Scientific reports. 2017; 7(1): 13319. [DOI:10.1038/s41598-017-13825-y] [PMID] [PMCID]

27. Sharifi S, Ghotaslo R, Aki MT, Soroush MH, Ansarian Kh, Shabanpour J, et al. Identification of respiratory infections caused by Mycoplasma pneumoniae With three methods of cultivation, ELISA and PCR . Medical journal of Tabriz University. 2011; 33(3): 36-41.

28. Dash S, Chaudhry R, Dhawan B, Dey AB, Kabra SK, Das BK. Clinical spectrum and diagnostic yields of Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia. J Lab Physicians. 2018; 10(1): 44-49. [DOI:10.4103/JLP.JLP_62_17] [PMID] [PMCID]

29. Zhou Y, Zhang Y, Sheng Y, Zhang L, Shen Z, Chen Z. More complications occur in macrolide-resistant than in macrolide-sensitive Mycoplasma pneumoniae pneumonia. Antimicrobial agents and chemotherapy. 2014; 58(2): 1034-8. [DOI:10.1128/AAC.01806-13] [PMID] [PMCID]

30. Morozumi M, Hasegawa K, Kobayashi R, Inoue N, Iwata S, Kuroki H, et al. Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation. Antimicrobial agents and chemotherapy. 2005; 49(6): 2302-6. [DOI:10.1128/AAC.49.6.2302-2306.2005] [PMID] [PMCID]

31. Xiao L, Ptacek T, Osborne JD, Crabb DM, Simmons WL, Lefkowitz EJ, et al. Comparative genome analysis of Mycoplasma pneumoniae. BMC Genomics. 2015; 16(1): 610. [DOI:10.1186/s12864-015-1801-0] [PMID] [PMCID]