Distribution of the Pathogenicity Island (PAI) Markers in Phylogenetic Groups of Uropathogenic Escherichia coli Collected from Hospitalized Patients in Zabol Hospitals

sholibor, Marzieh; rashki, ahmad; Rashki Ghalehnoo, Zahra

doi:10.30699/ijmm.12.5.311

year 12, Issue 5 (November - December 2018) Iran J Med Microbiol 2018, 12(5): 311-318 | Back to browse issues page

‎ 10.30699/ijmm.12.5.311

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sholibor M, rashki A, Rashki Ghalehnoo Z. Distribution of the Pathogenicity Island (PAI) Markers in Phylogenetic Groups of Uropathogenic Escherichia coli Collected from Hospitalized Patients in Zabol Hospitals . Iran J Med Microbiol 2018; 12 (5) :311-318
URL: http://ijmm.ir/article-1-836-en.html

Distribution of the Pathogenicity Island (PAI) Markers in Phylogenetic Groups of Uropathogenic Escherichia coli Collected from Hospitalized Patients in Zabol Hospitals

Marzieh Sholibor¹

, Ahmad Rashki

², Zahra Rashki Ghalehnoo³

1- Department of Biology, Faculty of Sciences, Zabol University, Zabol, Iran
2- Department of Pathobiology, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran , ah_rashki@usal.es
3- Department of Microbiology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran

Abstract: (5904 Views)

Background and Aims: Uropathogenic Escherichia coli (UPEC) are a causative agent in most of the urinary tract infections (UTIs) contain pathogenic island (PAI) which expresses a multitude of virulence factors. There is not much information about the type and distribution of these islands in the phylogenic groups of E. coli causing UTIs in different regions of Iran. In this study, the distribution of the pathogenicity island (PAI) markers infections was investigated among phylogenic groups of E. coli isolates collected from patients with UTIs using Multiplex-PCR method.
Materials and Methods: In this descriptive study, 100 isolates of E. coli collected from previous studies were conducted to determine the frequency of pathogenic islands and their distribution among phylogenic groups. In this method, genomic DNA of isolates was extracted by boiling method. Determination of the frequency of pathogenic islands was performed using Multiplex-PCR method. The results were analyzed using Fisher’s exact test.
Results: The prevalence of PAI IV₅₃₆, PAI I_CFT073, PAI II_CFT073, PAI II₅₃₆ and PAI II_J96 was 84%, 44%, 30%, 16% and 9%, respectively. The PAI I₅₃₆ and PAI I_J96 pathogens were not observed in any of the isolates. The highest distribution of PAI IV₅₃₆ island between phylogenetic groups B₂ and D was 88% (46 out of 52) and 100% (19 out of 19), respectively.
Conclusions: In this study, isolates belonging to groups B₂ and D were found to be the most pathogenic islands; therefore, they could play a more effective role in urinary tract infection than other phylogenic groups.