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:: year 12, Issue 2 (May - June 2018) ::
Iran J Med Microbiol 2018, 12(2): 0-0 Back to browse issues page
Evaluation Eucalyptus camaldulensis Contaminations with Cryptococcus gattii in Tehran Provience, Iran
Roohollah Fateh Dr1, Mahnaz Dehghani pour Mrs2, Farideh Zaini Dr3, Maasoumeh Mahdavi-Ourtakand Dr4, Zahra Salehi Dr5, Mona Ghazanfari Mrs6, Shirin Farahyar Dr7, Samira Salari Dr8, Azam Fattahi Dr 9
1- Professor Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
2- Professor Department of Biology, college of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
3- Professor Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4- professor Department of Biology, college of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
5- Student Department of Mycology, Faculty of Medicine, Tarbiat Modarress University, Tehran, Iran
6- ms Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
7- Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
8- Department of Medical Mycology and Parasitology, School of Medicine, Medical University of Kerman, Kerman, Iran
9- Professor Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran , fattahiazam63@gmail.com
Abstract:   (273 Views)
Background: Cryptococcosis is an infection caused by Cryptococcus species . Cryptococcus gattii  is mostly isolated from Eucalyptus trees and is acquired via inhalation of basidiospores. The present study was performed to isolate Cryptococcus sp. from Eucalyptus trees and evaluate C. gatti contamination of the Eucalyptus camaldulensis trees in some parks of Tehran and Varamin.
Materials and Methods: 88 trees (leaves, stalk, fruit and soil) were collected from different area of Tehran, Varamin and Shahr Ray over a period of 2 years during spring and fall. Identification of Cryptococcus sp. were performed based on colony color on niger seed agar medium, urease production, india ink test and pseudohyphae formation on corn meal agar with tween 80. Specific differentiation of Cryptococcus sp. were performed using sugar assimilation by API 20C AUX, disk approaches, colony color on canavanine glycine bromothymol blue and cycloheximide-phenol red agar medium .
Results and Discussion: 6 out of 88 samples of Eucalyptus trees were identified as Cryptococcus sp. Accordingly, Four isolates were identified as Cryptococcus albidus while two other isolates were not detectable by used methods. Although Cryptococcus gattii was not isolated from the collected samples, this does not mean that Eucalyptus trees in the province of Tehran are not contaminated.
Keywords: Cryptococcus Species, Niger Seed Agar Medium, Sugar Assimilation, Eucalyptus Camaldulensis
     
Type of Study: Original | Subject: Medical Mycology
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Fateh R, Dehghani pour M, Zaini F, Mahdavi-Ourtakand M, Salehi Z, Ghazanfari M, et al . Evaluation Eucalyptus camaldulensis Contaminations with Cryptococcus gattii in Tehran Provience, Iran. Iran J Med Microbiol. 2018; 12 (2)
URL: http://ijmm.ir/article-1-816-en.html


year 12, Issue 2 (May - June 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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