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:: year 12, Issue 5 (November - December 2019) ::
Iran J Med Microbiol 2019, 12(5): 329-337 Back to browse issues page
The Study of Antibiotic Resistance and Prevalence of Extended-Spectrum Beta-Lactamase (ESBLs) Encoding Genes in Acinetobacter baumanni Isolates from Raw Foodstuffs
Mohammad Moradi Mr1, Mohammad Reza Arabestani Dr1, Ghodratollah Roshanaii Dr2, Mohammad Yousef Alikhani Dr 3
1- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
3- Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , Alikhani43@yahoo.com
Abstract:   (447 Views)
 
Background and Aims: Pharmaceutical residuals like antibiotics in livestock products and their consumption by humans from food chain can lead to the spread of bacteria resistant to antibiotics. The aim of the current study was to investigate antibiotic resistance and determine the prevalence rate of genes encoding extended spectrum beta-lactamase (ESBLs) in Acintobacter strains isolated from raw foodstuffs.
Materials and Methods: In this study, 300 samples from protein foodstuffs (mutton, beef, chicken, hamburger, hot dog, sausages) and dairy foodstuffs (raw milk and cheese) were prepared and investigated in terms of contamination with Acinetobacter baumannii from July 2015 to November 2016. The isolated bacteria were identified by biochemical tests to species level and confirmed by the PCR technique via blaOXA-51 gene. Antibiotic resistance of the isolates was studied using the diffusion disc method. Also, the presence of ESBLs enzymes in the isolates was done phenotypically and genetically through PCR and combined disk tests.
Results: The results showed that 43 strains of A. baumannii were isolated from the protein and dairy foodstuffs, 93% from protein foodstuffs and 7%  from dairy foodstuffs. Also, 30% of the isolates had multidrug- resistance (MDR). Further, the findings of PCR illustrated that the prevalence of genes encoding ESBLs in the isolates were: TEM 21%, PER 23.5%, VEB 18.5% and SHV35%.
 Conclusions: Foodstuffs can act as a food source for A. baumannii that can lead to transferring and spreading genes encoding antibiotic resistance to humans.
Keywords: Acinetobacter baumannii, Antibiotic resistance, Extended-Spectrum Beta-Lactamase ‌
     
Type of Study: Original | Subject: Food Microbiology
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moradi M, Arabestani M R, Roshanaii G, Alikhani M Y. The Study of Antibiotic Resistance and Prevalence of Extended-Spectrum Beta-Lactamase (ESBLs) Encoding Genes in Acinetobacter baumanni Isolates from Raw Foodstuffs . Iran J Med Microbiol. 2019; 12 (5) :329-337
URL: http://ijmm.ir/article-1-764-en.html


year 12, Issue 5 (November - December 2019) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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