year 11, Issue 5 (November - December 2017)
Iran J Med Microbiol 2017, 11(5): 98-106 |
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Tajedini E, Talebi S, vandyousefi J. Evaluation and Comparison of ELISA and Indirect Immunofluorescence Methods for the Detection of Anti-Listeria Antibodies Among Women with Spontaneous Abortion Referred to Kamali Hospital in Karaj, Iran
. Iran J Med Microbiol 2017; 11 (5) :98-106
URL: http://ijmm.ir/article-1-743-en.html
URL: http://ijmm.ir/article-1-743-en.html
1- Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran ,romin.talebi.1@gmail.com
3- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran ,
3- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
Abstract: (7599 Views)
Background and Aims: Listeria monocytogenes is one of the most important causes of abortion and postpartum infection in newborns. This study aimed to compare the indirect ELISA and immunofluorescence methods for the detection of anti-Listeria antibodies among women with spontaneous abortion.
Materials and Methods: In this study, indirect ELISA and immuno-fluorescence methods were used for the detection of anti-Listeria antibodies. Five milliliters of blood sample were collected from mothers with spontaneous abortion during the third trimester of pregnancy in a ward that examines the diseases of female genital tract in Kamali Hospital of Karaj, Iran. The samples were then transferred to the laboratory for examination using relevant kits. The serum titers of positive samples were also assessed via both ELISA and indirect immunofluorescence for evaluation of IgG and IgM antibodies. The antibodies titers were compared using SPSS software while P values <0.001 were considered as significant.
Results: From the 58 evaluated serum samples, 21 were positive for anti-Listeria antibody. Statistical analysis showed that ELISA and indirect immunofluorescence methods had identical sensitivity. Moreover, it was shown that identification of IgG antibody was better performed in both diagnostic methods.
Conclusions: Both ELISA and indirect immunofluorescence methods are suitable methods for the detection of antibodies against Listeria.
Materials and Methods: In this study, indirect ELISA and immuno-fluorescence methods were used for the detection of anti-Listeria antibodies. Five milliliters of blood sample were collected from mothers with spontaneous abortion during the third trimester of pregnancy in a ward that examines the diseases of female genital tract in Kamali Hospital of Karaj, Iran. The samples were then transferred to the laboratory for examination using relevant kits. The serum titers of positive samples were also assessed via both ELISA and indirect immunofluorescence for evaluation of IgG and IgM antibodies. The antibodies titers were compared using SPSS software while P values <0.001 were considered as significant.
Results: From the 58 evaluated serum samples, 21 were positive for anti-Listeria antibody. Statistical analysis showed that ELISA and indirect immunofluorescence methods had identical sensitivity. Moreover, it was shown that identification of IgG antibody was better performed in both diagnostic methods.
Conclusions: Both ELISA and indirect immunofluorescence methods are suitable methods for the detection of antibodies against Listeria.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2017/07/29 | Accepted: 2017/10/9 | ePublished: 2017/11/20
Received: 2017/07/29 | Accepted: 2017/10/9 | ePublished: 2017/11/20
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