Bioaccumulation of Vitamin D3 by Lactobacillus plantarum and Optimization with Response Surface Methodology

Mohajeri Amiri, Morteza; Fazeli, Mohammad Reza; Samadi, Nasrin; Amini, Mohsen; Hayati Roodbari, Nasim

year 11, Issue 4 (September - October 2017) Iran J Med Microbiol 2017, 11(4): 35-44 | Back to browse issues page

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Mohajeri Amiri M, Fazeli M R, Samadi N, Amini M, Hayati Roodbari N. Bioaccumulation of Vitamin D3 by Lactobacillus plantarum and Optimization with Response Surface Methodology. Iran J Med Microbiol 2017; 11 (4) :35-44
URL: http://ijmm.ir/article-1-706-en.html

Bioaccumulation of Vitamin D3 by Lactobacillus plantarum and Optimization with Response Surface Methodology

Morteza Mohajeri Amiri¹

, Mohammad Reza Fazeli²

, Nasrin Samadi

³, Mohsen Amini⁴

, Nasim Hayati Roodbari¹

1- Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
2- Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran , samadin@tums.ac.ir
4- Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design, Tehran University of Medical Sciences, Tehran, Iran

Abstract: (13412 Views)

Background and Aims: Vitamin D3 deficiency can causes many diseases such as rickets, osteopenia and osteoporosis and increases the risk of some types of cancer. Probiotic strain of Lactobacillus plantarum, which can store fat-soluble vitamin D3 in its bulk, can reduce the effects of vitamin D3 deficiency in addition to being able to produce products with probiotic benefits..
Materials and Methods: This research was carried out in 2016. By designing the one-factor-at-a- time tests, the range of possible effective variables on vitamin D3 absorption in bacterial mass and effective factors were selected. Optimization of vitamin D3 entrapment in biomass of bacteria was performed using response surface methodology via Box-Behnken design. The high-performance liquid chromatography was employed for determination of vitamin D3 quantities.
Results: Among the parameters affecting vitamin D3 entrapment, three factors including incubation temperature, initial vitamin D3 and sucrose concentrations were most effective. The optimal points were obtained at vitamin D3 concentration of 351723.537 IU/mL, sucrose concentration of 2.89 (g/L) and incubation temperature of 33.8 °C. The maximum value of vitamin D3 in dry cell weight of L. plantarum was 1028.5 IU/g which was consistent with the proposed statistical model.
Conclusions: In this study L. plantarum enriched with vitamin D3 was produced and optimized for the first time. Experimental and statistical studies confirm the accuracy and reliability of this optimization.

Keywords: Lactobacillus plantarum, Vitamin D3, Box-Behnken design, Response surface methodology

Full-Text [PDF 705 kb] (3454 Downloads)

Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2017/05/14 | Accepted: 2017/06/18 | ePublished: 2017/09/12

References

1. Fitzpatrick TB, Basset GJC, Borel P, Carrari F, DellaPenna D, Fraser PD, et al. Vitamin Deficiencies in Humans: Can Plant Science Help? Plant Cell 2012; 24 (2):395–414. [DOI] [PubMed]

2. Medardus JJ, Molla BZ, Nicol M, Morrow WM, Rajala-Schultz PJ, Kazwala R, et al. In-Feed Use of Heavy Metal Micronutrients in U.S. Swine Production Systems and Its Role in Persistence of Multidrug-Resistant Salmonellae. Appl Environ Microbiol 2014; 80 (7):2317–25. [DOI] [PubMed]

3. Hemarajata P, Versalovic J. Effects of probiotics on gut microbiota: mechanisms of intestinal immunomodulation and neuromodulation. Therap Adv Gastroentrol 2013; 6 (1):39–51. [DOI] [PubMed]

4. Chau YY, Kumar J. Vitamin D in Chronic Kidney Disease. Ind J Pediatr 2012; 79(8):1062–8. [DOI] [PubMed]

5. Simm C, Lahner B, Salt D, LeFurgey A, Ingram P, Yandell B, et al. Saccharomyces cerevisiae vacuole in zinc storage and Intracellular zinc distribution. Eukaryot Cell 2007; 6 (7):1166–77. [DOI] [PubMed]

6. Farazandehnia N. The evaluation of antimicrobial effect of fermented probiotic milk produced Lactobacillus casei, Lactobacillus plantarum, Bifidobacterium and Bifidobacterium angultum. Iran J Med Microbiol 2016; 10 (3):31-8.

7. Ershadian M, Arbab Soleimani N, Ajodani Far H, Vaezi Khakhki M R. The Co-aggregation effects of probiotic lactobacillus against some pathogenic bacteria. Iran J Med Microbiol 2015; 9 (3):14-22.

8. Soltani M, Soltani J. Determination of optimal combination of applied water and nitrogen for potato yield using response surface methodology (RSM). J Biosci Biotechnol Res Comm 2016; 9 (1):46-54.

9. Popović S, Kostadinović LM, Brkljača JS, Krulj JA, Manojlović MS, Solarov MIB. The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa Willd.). Food Feed Res 2014; 41 (2):147–52. [DOI]

10. Stehlik-Tomas V, Gulan Zeti V, Stanzer D, Grba S, Vahcic N. Zinc, Copper and Manganese enrichment in yeast Saccharomyces cerevisiae. Food Technol Biotechnol 2004; 42 (2):115-20.

11. Olsen RH, Thomas D. Characteristics and purification of PRRI, an RNA phage specific for the broad host range Pseudomonas RI 822 drug resistance plasmid. J Virol 1973; 12 (6):1560-7. [PubMed]

12. Olsen RH, Shipley P. Host range and properties of the Pseudomonas aeruginosa R factor RI 822. J Bacteriol 1973; 113 (2):772-80. [PubMed]

13. Smet P, Boever D, Verstraete W. Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity. Br J Nutr 1998; 79 (2):185–94. [DOI] [PubMed]

14. Hwang CF, Chang JH, Houng JY, Tsi CC, Lin CK, Tsen HY. Optimization of Medium Composition for Improving Biomass Production of Lactobacillus plantarum Pi06 Using the Taguchi Array Design and the Box-Behnken Method. Biotechnol Bioprocess Engin 2012; 17 (4):827-34. [DOI]

15. Leal-Sa´nchez MV, Jime´nez-Díaz R, Maldonado-Barraga´n A, Garrido-Ferna´ndez A, Ruiz-Barba JL. Optimization of Bacteriocin Production by Batch Fermentation of Lactobacillus plantarum LPCO10. Appl Environ Microbiol 2002; 68 (9):4465–71. [DOI] [PubMed]

16. Keren T, Yarmus M, Halevy G, Shapyra R. Immunodetection of the Bacteriocin Lacticin RM: Analysis of the Influence of Temperature and Tween 80 on Its Expression and Activity. Appl Environ Microbiol 2004; 70 (4):2098–104. [DOI] [PubMed]

17. Sanchez JI, Martı´nez B, Guille´n R, Jime´nez-Dı´az R, Rodrı´guez A. Culture Conditions Determine the Balance between Two Different Exopolysaccharides Produced by Lactobacillus pentosus LPS26. Appl Environ Microbiol 2006; 72 (12):7495–502. [DOI] [PubMed]

18. Pedram A, Ataei SA. Optimization of a Modified GS Medium for a Probiotic Strain (L. acidophilus ATCC4356). Appl Food Biotechnol 2014; 1 (1):25-9.

19. Brinques GB, Peralba MC, Ayub MAZ. Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems. J Ind Microbiol Biotechnol 2010; 37(2): 205–12. [DOI] [PubMed]

20. Brzozowski B, Lewandowska M. Prolyl endopeptidase —Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus. Electron J Biotechnol 2014; 17 (5):204–10. [DOI]

21. Thirumurugan A, Ramachandran S, Gobikrishnan S. Optimization of medium components for maximizing the bacteriocin production by Lactobacillus plantarum ATM11 using statistical design. Int Food Res J 2015; 22 (3):1272-9.