Isolation of lytic phages against pathogenic E.coli isolated from diabtic ulcers

Zare, leyla; Shenagari, Mohammad; Khanmirzaei, Mohammad Ali; Mojtahedi, Ali

year 11, Issue 2 (May - June 2017) Iran J Med Microbiol 2017, 11(2): 34-41 | Back to browse issues page

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Zare L, Shenagari M, Khanmirzaei M A, Mojtahedi A. Isolation of lytic phages against pathogenic E.coli isolated from diabtic ulcers. Iran J Med Microbiol 2017; 11 (2) :34-41
URL: http://ijmm.ir/article-1-701-en.html

Isolation of lytic phages against pathogenic E.coli isolated from diabtic ulcers

Leyla Zare¹

, Mohammad Shenagari

², Mohammad Ali Khanmirzaei³

, Ali Mojtahedi¹

1- Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran , shenagari@gmail.com
3- Department of Microbiology and Immunology, McGill University, Montréal, Quebec, Canada

Abstract: (11324 Views)

Background and Aim: In the present study, we have tried to isolate an effective lytic bacteriophages on drug-resistant Escherichia coli isolated from diabetic ulcer wounds.

Materials and Methods: Two E.coli strains were isolated by using standard methods from patients in Razi Hospital of Rasht. Waste water of the same hospital was used as a source for isolation of lytic bacteriophages. Agar layer method was used to isolate and plaque finding. Determination of host range of isolated phages was performed using five standard reference collection of E. coli. Finally, Transmission electron microscopy was used for morphological analysis of the isolated bacteriophages. Morphotype and family of isolated phages were determined based on Bradley recommendation and final version of International Committee on Taxonomy of Viruses (ICTV) report, respectively.

Results: The results showed that the isolated bacteria were highly resistant to the antibiotics tested. Based on morphological characteristics, the separated virus belongs to siphoviridae family. Based on Bradley classification, isolated phage against strains of E.coli belongs to the morphotype B1.

Conclusions: The isolated bacteriophage from waste water efficiently lysed the multiresistant strains of E. coli. Therefore, bacteriophages could potentially be used as an alternative for antibiotics for treating infections by multiresistant E. coli.

Keywords: Bacteriophage, Antibiotic Resistance, Escherichia coli, Diabetes, Wound infection

Full-Text [PDF 736 kb] (5106 Downloads)

Type of Study: Original Research Article | Subject: Medical Virology
Received: 2017/05/8 | Accepted: 2017/05/30 | ePublished: 2017/06/7

References

1. Bruttin A, Brussow H. Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy. Antimicrob Agents Chemother 2005;49(7):2874–8. [PubMed]

2. Dufour N, Debarbieux L, Fromentin M, Ricard JD. Treatment of highly virulent extraintestinal pathogenic Escherichia coli pneumonia with bacteriophages. Crit Care Med 2015;43(6):190–8. [PubMed]

3. Abedon ST, Kuhl SJ, Blasdel BG, Kutter EM. Phage treatment of human infections. Bacteriophage 2011;1(2):66-85. [PubMed]

4. Renvall S, Niinikoski J, Aho AJ. Wound infections in abdominal surgery. A prospective study on 696 operations. Acta Chir Scand 1980; 146(1):25-30 [PubMed]

5. Petkovšek Ž, Elersic K, Gubina M, Zgur-Bertok D, Starcic Erjavec M. Virulence potential of Escherichia coli isolates from skin and soft tissue infections. J Clin Microbiol 2009;47(6): 1811-7. [PubMed]

6. Aghili R, Malek M, Baradaran HR, Peyvandi AA, Ebrahim Valojerdi A, Khamseh ME. General Practitioners' Knowledge and Clinical Practice in Management of People with Type 2 Diabetes in Iran; The Impact of Continuous Medical Education Programs. Arch Iran Med 2015; 18(9):582-5. [PubMed]

7. Boykoe EJ, Lipsky BA. In: Diabetes in America. Harris MI, editor. Washington DC: National Institutes of Health. Infection and diabetes mellitus; 1995 pp. 485–496.

8. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum β-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. J Glob Antimicrob Resist 2016; 7:110-3. [PubMed]

9. Sulakvelidze A. Bacteriophage therapy. Antimicrob Agents Chemother 2001; 45(3):649-659. [PubMed]

10. Ackermann HW. Phage Classification and Characterization. Methods Mol Biol 2009;501:127-40. [PubMed]

11. Ackermann, HW. 5500 Phages examined in the electron microscope. Arch Virol 2007; 152(2):227-243. [PubMed]

12. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Fifth Informational Supplement. M100-S25. Wayne, Pennsylvania, USA—January 2015

13. Krupovic M, Dutilh BE, Adriaenssens EM, WittmannJ, Vogensen FK, Sullivan MB, et al. Taxonomy of prokaryotic viruses: update from the ICTV bacterial and archaeal viruses subcommittee. Arch Virol 2016;4:1095–9. [PubMed]

14. Bradley D. Ultrastructure of Bacteriophages and Bacteriocins. Bacteriological Reviews. 1967; 31(4): 230-314. [PubMed]

15. Ochman H, Selander RK. Standard reference strains of Escherichia coli from natural populations. J Bacteriol 1984;157(2):690-3. [PubMed]

16. Chain E, Florey HW, Gardner AD, Heatley NG. Penicillin as a chemotherapeutic agent. Lancet 1940; 236(6104):226–8. [Article]

17. Sulakvelidze A. The challenges of bacteriophage therapy. Eur Ind Pharm 2010;10:14-18. [Article]

18. Hagens S, Loessner MJ. Application of bacteriophages for detection and control of foodborne pathogens. Appl Microbiol Biotechnol 2007; 76(3):513-9. [PubMed]

19. Mahony D, Clow J, Atkinson L, Vakharia N, Schlech W. Development and application of a multiple typing system for Clostridium difficile. Appl Environ Microbiol 1991; 57:1873–9. [PubMed]

20. Slopek S, Durlakowa I, Weber-Dabrowska B, Kucharewicz-Krukowska A, Dabrowski M, Bisikiewicz R. Results of bacteriophage treatment of suppurative bacterial infections. I. General evaluation of the results. Arch Immunol Ther Exp 1983; 31:267–291. [PubMed]

21. Smith HW, Huggins M. Successful treatment of experimental Escherichia coli infections in mice using phage: its general superiority over antibiotics. Microbiology 1982;128(2):307-318. [PubMed]

22. Moş I, Micle O, Zdranca M, Muresan M, Vicas L. Antibiotic sensitivity of the Escherichia coli strains isolated from infected skin wounds. Farmacia 2010;58(5):637-644. [Article]

23. Biswas B, Adhya S,Washart P, Paul B, Trostel AN, Powel B, et al. Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium. Infect Immun 2002;70(1):204-210. [PubMed]