Study of Lipase genes expression (Lip2,3,5) in Candida albicans isolated from patients

Ghaffari, Mansoureh; Noorbakhsh, Fatemeh; Kachuei, Reza

year 11, Issue 2 (May - June 2017) Iran J Med Microbiol 2017, 11(2): 53-60 | Back to browse issues page

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Ghaffari M, Noorbakhsh F, Kachuei R. Study of Lipase genes expression (Lip2,3,5) in Candida albicans isolated from patients . Iran J Med Microbiol 2017; 11 (2) :53-60
URL: http://ijmm.ir/article-1-672-en.html

Study of Lipase genes expression (Lip2,3,5) in Candida albicans isolated from patients

Mansoureh Ghaffari¹

, Fatemeh Noorbakhsh

², Reza Kachuei³

1- Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva, Iran
2- Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva, Iran , niloofar_noorbakhsh@yahoo.com
3- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Abstract: (10728 Views)

Background and Aims: Candida, is a fungal flora in human that can be colonized on the skin and mucosal surfaces. Various factors are involved in causing disease by candida, one of the most important of these factors, are secreted hydrolytic enzymes and aspartyl proteinase enzymes are as a potential factor, but other secretion of hydrolytic enzymes such as phospholipase and lipase are also important pathogen factors. In this study, gene expression of LIP2, LIP3 and LIP5 were studied in isolated C. albicans in 100 samples from patients.

Materials and Methods: A total of 100 strains of C. albicans was isolated in 2015-2016 years by morphology and molecular methods, from clinical samples. After extracting RNA from them by RNX-Plus kit, expression of LIP2, LIP3 and LIP5genes was evaluated by using RT-PCR test.

Results: From 100 clinical isolates of C. albicans, LIP2 gene expressed 55%, LIP3 gene 41% and LIP5 gene 48%. Among the positive samples, 24 cases (33.80%) were expressed three genes at the same time.

Conclusions: In this study, frequency of lipase genes was different in strains of C.albicans. Lipase gene was not expressed in 30% of cases. It can be concluded that other genes than lipase gene can be involved in the pathogenesis.

Keywords: Candida albicans, Lipase encoding genes, LIP2, LIP3, LIP5, RT-PCR

Full-Text [PDF 683 kb] (3023 Downloads)

Type of Study: Original Research Article | Subject: Medical Mycology
Received: 2017/02/8 | Accepted: 2017/05/28 | ePublished: 2017/06/7

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