Detection of LMP1 protein in Breast Cancer tissue samples from Fars province hospitals

Shariat, Afsoon

year 11, Issue 1 (March - April 2017) Iran J Med Microbiol 2017, 11(1): 67-74 | Back to browse issues page

Mendeley

Zotero

RefWorks

Shariat A. Detection of LMP1 protein in Breast Cancer tissue samples from Fars province hospitals . Iran J Med Microbiol 2017; 11 (1) :67-74
URL: http://ijmm.ir/article-1-590-en.html

Detection of LMP1 protein in Breast Cancer tissue samples from Fars province hospitals

Afsoon Shariat

Department of Microbiology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran , afsoonsh1980@yahoo.com

Abstract: (8472 Views)

Background and Aim: Breast cancer is the most common malignancy among women and Epstein Barr virus could be a reason to develop this cancer. The aim of this study is to evaluate the association between Epstein Barr virus and breast cancer by Immunohistochemical method in Fars province hospitals.

Materials and Methods: This study was carried out in 1390-1395. 80 cases of breast cancer tissue samples were selected from formalin-fixed paraffin embedded blocks from Fars province hospitals and by immunohistochemistry method the expression of the LMP1 viral protein were examined. Data analyzed by SPSS statistical software.

Results: Epstein Barr virus was not detected in the breast cancer tissues and expression of viral protein of LMP1 was not observed in any of the breast cancer specimens by Immunohistochemistry.

Conclusions: The results were shown that Epstein Barr virus may not be associated with breast cancer in Fars province and further studies are needed to determine the relationship between breast cancer virus with a variety of molecular methods.

Keywords: Epstein-Barr virus, Breast cancer, Immunohistochemistry, LMP1 protein

Full-Text [PDF 615 kb] (2299 Downloads)

Type of Study: Original Research Article | Subject: Medical Virology
Received: 2016/07/16 | Accepted: 2016/11/19 | ePublished: 2017/03/16

References

1. Zekri AR, Bahnassy AA, Mohamed WS, El-Kassem FA, El-Khalidi SJ, Hafez MM, et al. Epstein-Barr virus and breast cancer: Epidemiological and Molecular study on Egyptian and Iraqi women. J Egypt Natl Canc Inst 2012; 24(3):123-31. [Article]

2. Mohammadizadeh F, Zarean M, Abbasi M. Association of Epstein-Barr virus with invasive breast carcinoma and its impact on well-known clinicopathologic parameters in Iranian women. Adv Biomed Res 2014; 3(1):141. [PubMed]

3. Bonnet M, Guinebretiere JM, Kremmer E, Grunewald V, Benhamou E, Contesso G, et al. Detection of Epstein-Barr Virus in Invasive Breast Cancers. J Natl Cancer Inst 1999; 91(16):1376–81. [PubMed]

4. Ahmed RA, Yussif SM. Immunohistochemical detection of human cytomegalovirus, Epstein-Barr virus and human papillomavirus in invasive breast carcinoma in Egyptian women: A tissue microarray study. JST 2016; 6(2): 8-16. [Article]

5. Khabaz MN. Association of Epstein-Barr virus infection and breast Carcinoma. Arch Med Sci 2013; 9(4):745–51. [PubMed]

6. Glaser SL, Hsu JL, Gulley ML. Epstein-Barr Virus and Breast Cancer: State of the Evidence for Viral Carcinogenesis. Cancer Epidemiol Biomarkers Prev 2004; 13(5):688–97. [PubMed]

7. Aboulkassim T, Yasmeen A, Akil N, Batist G, Moustafa AE. Incidence of Epstein–Barr virus in Syrian women with breast cancer: A tissue microarray study. Hum Vaccin Immunother 2015;11(4):951-5. [PubMed]

8. Tabyaoui I, Serhier Z, Sahraoui S, Sayd S, Cadi R, Bennani OM, et al. Immunohistochemical expression of latent membrane protein 1 (LMP1) and p53 in nasopharyngeal carcinoma: Moroccan experience. Afr Health Sci 2013; 13(3):710–17. [PubMed]

9. Arbach H, Viglasky V, Lefeu F, Guinebretiere JM, Ramirez V, Bride N, et al. Epstein-Barr Virus (EBV) Genome and Expression in Breast Cancer Tissue: Effect of EBV Infection of Breast Cancer Cells on Resistance to Paclitaxel (Taxol). J Virol 2006; 80(2):845–53. [Article]

10. Mant C, Hodgson S, Hobday R, D’Arrigo C, Cason J. A viral aetiology for breast cancer: time to re-examine the postulate. Intervirology 2004;47(1):2-13. [PubMed]

11. Lawson JS, Gunzburg WH, Whitaker NJ. Viruses and human breast cancer. Future Microbiol 2006; 1(1):33-51. [PubMed]

12. Lespagnard L, Cochaux P, Larsimont D, Degeyter M, Velu T, Heimann R. Absence of Epstein-Barr virus in medullary carcinoma of the breast as demonstrated by immunophenotyping, in situ hybridization and polymerase chain reaction. Am J Clin Pathol 1995; 103(4):449-52. [PubMed]

13. Chu JS, Chen CC, Chang KJ. In situ detection of Epstein-Barr virus in breast cancer. Cancer Lett 1998; 124(1):53-7. [PubMed]

14. Luqmani YA, Shousha S. Presence of Epstein-Barr virus in breastcarcinoma. Int J Oncol 1995; 6(4):899-903. [PubMed]

15. Deshpande CG, Badve S, Kidwai N, Longnecker R. Lack of expression of the Epstein-Barr virus (EBV) gene products, EBERs, EBNA1, LMP1, and LMP2A, in breast cancer cells. Lab Investig 2002; 82(9):1193–9. [PubMed]

16. Herrmann K, Niedobitek G. Lack of evidence for an association of Epstein-Barr virus infection with breast carcinoma. Breast Cancer Res 2003; 5(1):R13–R17. [Article]

17. Murray PG, Lissauer D, Junying J, Davies G, Moore S, Bell A, et al. Reactivity with a monoclonal antibody to Epstein-Barr virus (EBV) nuclear antigen 1 defines a subset of aggressive breast cancers in the absence of the EBV genome. Cancer Res 2003; 63(9):2338–43. [PubMed]

18. Perrigoue JG, den Boon JA, Friedl A, Newton MA, Ahlquist P, Sugden B. Lack of Association between EBV and Breast Carcinoma. Cancer Epidemiol Biomarkers Prev 2005; 14(4):809–14. [PubMed]

19. Joshi D, Buehring GC. Are viruses associated with human breast cancer? Scrutinizing the molecular evidence. Breast Cancer Res Treat 2012; 135(1):1-15. [PubMed]

20. Torabizadeh Z, Nadji A, Naghshvar F, Nosrati A, Parsa M. Association between Epstein - Barr Virus (EBV) and Breast Cancer. Res Mol Med 2014; 2(4):24. [Article]

21. Kadivar M, Monabati A, Joulaee A, Hosseini N. Epstein-Barr virus and breast cancer: Lack of evidence for an association in Iranian women. Pathol Oncol Res 2011; 17(3):489-92. [PubMed]

22. McCall SA, Lichy JH, Bijwaard KE, Aguilera NS, Chu WS, Taubenberger JK. Epstein-Barr virus detection in ductal carcinoma of the breast. J Natl Cancer Inst 2001; 93(2):148-50. [Article]

23. Chu PG, Chang KL, Chen YY, Chen WG, Weiss LM. No significant association of Epstein-Barr virus infection with invasive breast carcinoma. Am J Pathol 2001; 159(2):571-8. [PubMed]

24. Cochet C, Martel-Renoir D, Grunewald V, Bosq J, Cochet G, Schwaab G, et al. Expression of the Epstein-Barr virus immediate early gene, BZLF1, in nasopharyngeal carcinoma tumor cells. Virology 1993; 197(1):358-65. [PubMed]

25. Xue SA, Lampert IA, Haldane JS, Bridger JE, Griffin BE. Epstein-Barr virus gene expression in human breast cancer: protagonist or passenger. Br J Cancer 2003; 89(1):113-19. [PubMed]

26. Fina F, Romain S, Ouafik L, Palmari J, Ayed FB, Benharkat S, et al. Frequency and genome load of Epstein-Barr virus in 509 breast cancers from different geographical areas. Br J Cancer 2001; 84(6):783–90. [PubMed]

27. Labrecque LG, Barnes DM, Fentiman IS, Griffin BE. Epstein Barr virus in epithelial cell tumors: a breast carcinoma study. Cancer Res 1995; 55(1):39-45. [PubMed]

28. Marrao G, Habib M, Paiva A, Bicout D, Fallecker C, Franco S, et al. Epstein-Barr virus infection and clinical outcome in breast cancer patients correlate with immune cell TNF-α/IFN-γ response. BMC Cancer 2014; 14(3):665. [PubMed]

29. Khan G, Philip PS, Ashari M. Is Epstein–Barr virus associated with aggressive forms of breast cancer.Br J Cancer 2011; 104(8):1362–3. [PubMed]

30. Lorenzetti MA, Matteo ED, Gass H, Vazquez PM, Lara J, Gonzalez P, et al. Characterization of Epstein Barr Virus Latency Pattern in Argentine Breast Carcinoma. PLoS One 2010; 5(10):e13603. [Article]

31. Huo Q, Zhang N, Yang Q. Epstein-Barr Virus Infection and Sporadic Breast Cancer Risk: A Meta-Analysis. PLoS One 2012; 7(2):e31656. [Article]

32. Richardson AK, Currie MJ, Robinson BA, Morrin H, Phung Y, Pearson JF, et al. Cytomegalovirus and Epstein-Barr Virus in Breast Cancer. PLoS One 2015; 10(2):e0118989. [Article]