Frequency determination of blaTEM & blaSHV in the Extended-Spectrum Beta-Lactamase producing Escherichia coli isolates from Karaj

Haddadi, Azam; Yeke fallah, Fatemeh

year 11, Issue 2 (May - June 2017) Iran J Med Microbiol 2017, 11(2): 75-80 | Back to browse issues page

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Haddadi A, Yeke fallah F. Frequency determination of blaTEM & blaSHV in the Extended-Spectrum Beta-Lactamase producing Escherichia coli isolates from Karaj. Iran J Med Microbiol 2017; 11 (2) :75-80
URL: http://ijmm.ir/article-1-579-en.html

Frequency determination of blaTEM & blaSHV in the Extended-Spectrum Beta-Lactamase producing Escherichia coli isolates from Karaj

Azam Haddadi

¹, Fatemeh Yeke fallah²

1- Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran , haddadi@kiau.ac.ir
2- Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract: (7879 Views)

Background and Aim: Extended-spectrum β-lactamases are groups of enzyme with the capability of hydrolyzing cephalosporins and aztreonam. Various types of extended-spectrum β-lactamases (ESBLs), such as bla_TEM and bla_SHV are present prominently in E. coli strains. This study aimed to determine the frequency of bla_TEM and bla_SHVgenes in the extended-spectrum β-lactamases, producing E. coli strains from urinary tract infections in Karaj city.

Materials and Methods: In this study which performed during the summer of 2015, antibiotic susceptibility pattern of 119 isolated E. coli strains from urinary tract infections were determined to 16 different antibiotics using Kirby-Bauer disk diffusion method. Extended-spectrum β-lactamases-producing bacteria were identified using double disc diffusion method by combination discs and were studied by using specific primers for genes bla_TEM and bla_SHV.

Results and Conclusions: The highest and lowest percentage of resistance to the antibiotic were tetracycline (61%) and nitrofurantoin (8.5%), respectively. 42.8% of isolates were extended-spectrum β-lactamases positive. Based on the PCR results the frequency of bla_TEMand bla_SHV were 60.78% and 39.21% respectively and 9.32% of isolates had both genes. High levels of genes bla_TEM and bla_SHV resistance to third generation of cephalosporins strains considered a problem. Diagnose of extended-spectrum β-lactamases strains in clinical laboratory also should be considered and it is recommended that prescription of cephalosporins should be restricted to susceptible isolates.

Keywords: Escherichia coli, Extended-spectrum β-lactamases, blaTEM, blaSHV

Full-Text [PDF 597 kb] (2236 Downloads)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2016/06/17 | Accepted: 2017/01/21 | ePublished: 2017/06/7

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