Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite

Nojoki, Fahimeh; Hatamian-Zarmi, Ashrafalsadat; Ebrahimi-Hosseinzadeh, Bahman; Mir-drikvand, Mohammad; Mokhtari-Hosseini, Zahra begom; Kalantari-Deahaghi, Saeid

year 11, Issue 1 (March - April 2017) Iran J Med Microbiol 2017, 11(1): 58-66 | Back to browse issues page

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Nojoki F, Hatamian-Zarmi A, Ebrahimi-Hosseinzadeh B, Mir-drikvand M, Mokhtari-Hosseini Z B, Kalantari-Deahaghi S. Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite. Iran J Med Microbiol 2017; 11 (1) :58-66
URL: http://ijmm.ir/article-1-572-en.html

Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite

Fahimeh Nojoki¹

, Ashrafalsadat Hatamian-Zarmi

², Bahman Ebrahimi-Hosseinzadeh¹

, Mohammad Mir-drikvand¹

, Zahra begom Mokhtari-Hosseini³

, Saeid Kalantari-Deahaghi¹

1- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran , hatamian_a@ut.ac.ir
3- Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering, Hakim Sabzevari University, Sabzevar, Iran

Abstract: (8519 Views)

Background and Aim: Ganoderma lucidum is a main source of ganoderic acid. This metabolite has pharmaceutical effects such as cancer treatment. However, due to low yields and high prices, clinical application is difficult. Stimulants such as elicitor and mechanical shocks can be used to increase the produce of ganoderic acid effectively. This study aimed to investigate the effect of ultrasound on the production of this medicine.

Materials and Methods: In this study, a liquid culture of the fungus treated with the once and twice at 20 and 200 seconds of sound with a frequency of 50 kHz and constant output power in ultrasonic bath was investigated. After extraction of ganoderic acid and ensure of positive impact of shock, to optimize variables, response surface methodology was used. In this method, 17 experiments with three variables, the first time ultrasound (4, 5, 6 days), the second ultrasound (7, 8, 9 days) and time of ultrasound (60, 180, 300 seconds) by Design Expert software were designed and done.

Results: The results showed that the effects of ultrasound stimulation with twice 274 seconds treatment on days 4 and 7 were established highest ganoderic acid production. By comparing samples with control (without sound) Increase in extracellular ganoderic acid to intracellular ratio was determined. In optimum conditions, 34% increase in GA production relative to the control sample was observed.

Conclusions: Ultrasound can be used as a non-chemical and cheap stimulus, increase ganoderic acid production.

Keywords: Ultrasound, Ganoderma lucidum, Ganoderic acid, Anticancer metabolite

Full-Text [PDF 911 kb] (3036 Downloads)

Type of Study: Original Research Article | Subject: Microbial Biotechnology
Received: 2016/06/3 | Accepted: 2016/09/30 | ePublished: 2016/10/17

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