Isolation and Identification of Acanthamoeba from Tonekabon Rivers Regional, Mazandaran

Mataji Bandpei, Shahin; Khataminejad, Mohammadreza

year 10, Issue 5 (November - December 2016) Iran J Med Microbiol 2016, 10(5): 60-66 | Back to browse issues page

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Mataji Bandpei S, Khataminejad M. Isolation and Identification of Acanthamoeba from Tonekabon Rivers Regional, Mazandaran. Iran J Med Microbiol 2016; 10 (5) :60-66
URL: http://ijmm.ir/article-1-563-en.html

Isolation and Identification of Acanthamoeba from Tonekabon Rivers Regional, Mazandaran

Shahin Mataji Bandpei¹

, Mohammadreza Khataminejad

1- Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
2- Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran , mr_khataminezhad@yahoo.com

Abstract: (8664 Views)

Background and Aim: Acanthamoeba species are abundant in soil and water samples and isolated from eye wash equipment sectors in medical laboratory. This single cell is as a cause of opportunistic infections, especially in patients with AIDS and organ recipients. The aim of this study was to isolate and identify the Acanthamoeba spp. from Tonekabon Rivers.

Materials and Methods: This study was a descriptive study. 100 samples were collected from the river in the Tonekabon city and were cultured on nutrient agar medium. Then Acanthamoeba to evaluate and identify common species identified using morphological and polymerase chain reaction (PCR) tests.

Results: 23 samples (23%) of the Acanthamoeba were identified morphologically and also using specific primers to amplify a 500 bp 18SrRNA. After sequencing, it was found that genotypes of Acanthamoeba, belongs to a new species, Acanthamoeba palestinensis.

Conclusions: The results show that A. palestinensis is one of the Amoebic keratitis risk factors in Tonekabon rivers. Health planners must take note and make people aware of the risk of contamination.

Keywords: Amoeba, Amoebic keratitis, Acanthamoeba palestinensis, Tonekabon rivers, PCR

Full-Text [PDF 742 kb] (3754 Downloads)

Type of Study: Original Research Article | Subject: Medical Parasitology
Received: 2016/05/18 | Accepted: 2016/07/14 | ePublished: 2016/10/17

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