Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province

Doosti Irani, Mehrnoosh; Faghani, Mostafa; Zia jahromi, Noosha

year 10, Issue 5 (November - December 2016) Iran J Med Microbiol 2016, 10(5): 38-44 | Back to browse issues page

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Doosti Irani M, Faghani M, Zia jahromi N. Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province. Iran J Med Microbiol 2016; 10 (5) :38-44
URL: http://ijmm.ir/article-1-540-en.html

Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province

Mehrnoosh Doosti Irani¹

, Mostafa Faghani

², Noosha Zia jahromi¹

1- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Department of Animal Sciences, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , mostafafaghani@yahoo.com

Abstract: (8282 Views)

Background and Aim: High prevalent of drug resistant in Salmonella is a threat to human’s health. Integrons are one of the most important factors that can contribute to the occurrence of MDR bacteria. The aim of this study was to determine the prevalence of class 1, 2 integrons among Salmonella strains isolated from broiler chicks.

Materials and Methods: This study was performed on 100 Salmonella i solated strains, collected from broiler chicks samples during the summer of 2015 in Chaharmahal va Bakhtiari. The prevalence of class 1and 2 integrons were verified using specific primers by multiplex PCR assay. Susceptibility to antimicrobial agents was tested using the disk diffusion method according to the CLSI 2011. In the end integrons prevalence and drug resistance were analyzed by using SPSS software.

Results: Screening of Salmonella isolates revealed the prevalence of class 1, 2 integrons (50%), (28%) and (48%), respectively. Based on the results of the antibiogram test, the highest rate of antibiotic resistance was Ampicillin among all isolates. The highest rate of antibiotic resistance in samples of IntI 1-positive have been to Gentamycin and Tetracycline (16%) and in IntI 2-positive have been to Gentamycin (35.7%). Also the intermediate resistant strains to Chloramphenicol in the samples of IntI 1-positive were 72% and in the samples of IntI 2-positive were 42.9%.

Conclusions: The presence of two classes of integrons and its direct connection with the MDR in Salmonella is concerned. Based on the results of this study, significant correlations were between MDR and integrons that are a serious problem in human and veterinary medicine.

Keywords: Salmonella, Integrons, Drug resistance, Broiler chicks

Full-Text [PDF 661 kb] (2299 Downloads)

Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2016/04/5 | Accepted: 2016/09/17 | ePublished: 2016/10/17

References

1. Asgharpour F, Rajabnia R, Ferdosi Shahandashti E, Marashi M, Khalilian M, Moulana Z. Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance. jjm 2014; 7(5). [in persion] [PubMed]

2. Latorre L, Parisi A, Fraccalvieri R, Normanno M, Nardella L, Goffredo E, Palazzo L, Ciccarese G, Santagada G. Low Prevalence of Listeria monocytogenes in food from Italy. J Food Prot 2007; 70(6): 1507-12. [PubMed]

3. Higashide W, Dai SH, Hombs VP, Zhou D. Involvement of sipA in modulating actin dynamics during salmonella invasion into cultured epithelial cells. Cell Microbiol 2002; 4(6): 357-65. [PubMed]

4. Desin T, Koster W, Potter A. Salmonella vaccines in poultry: past, present and future. Expert Rev Vaccines 2013; 12(1): 87-96. [PubMed]

5. Povilonis J, Seputienė V, Ruzauskas M, Siugzdiniene R, Virgailis M, Pavilonis A, Suziedeliene E. Transferable Class 1 and 2 Integrons in Escherichiacoli and Salmonella enterica Isolates of Human and Animal Origin in Lithuania. Foodborne Pathog Dis 2010; 7(10): 1185-1192. [PubMed]

6. Kargar M, Mohammadalipour Z, Doosti A, Lorzadeh SH, Japoni-Nejad A. High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran. Osong Public Health Res Perspect 2014; 5(4):193-198. [in persion] [PubMed]

7. Ranjbar R, Naghoni. Class 1 integron-mediated antibiotic resistance in Salmonella enterica strains isolated in Tehran, Iran. Iran J Med Microbiol 2014; 7(4): 16-23. [in persion] [Article]

8. Zakharyan MK, Sedrakyan AM, Arakelova KA, Hovannisyan AI, Asoyan AV, Gevorgyan ZU, Mnatsakanyan AA, Ktsoyan Zh A, Boyajyan AS, Aminov RI. Antibiotic Resistance and Occurrence of Class 1 Integrons in Clinical Isolates of Salmonella enterica.Glob. J Immunol Allerg Dis 2013; 1(2): 44-48.

9. Braden CR. Salmonella enterica Serotype Enteritidis and Eggs: A National Epidemic in the United States. Food safety 2006; 43:512–517. [PubMed]

10. Zeighami H, Haghi F, Hajiahmadi F. Integrons and Their role in Antibiotic Resistance. Labdiag J 2014; 5(22): 61-71. [in persion] [Article]

11. Domingues S, Silva GJ, Nielsen KM. Integrons: Vehicles and pathways for horizontal dissemination in bacteria. Mob Genet Elements 2012; (2)5: 211–223. [PubMed]

12. Dariushi M, Doosti A, Kargar M. The prevalence of plasmid genes spvB, spvC and spvR in Salmonella enteritidis isolated from poultry industry in Chaharmahal va Bakhtiari province. JMW 2015; 4(21): 282-288. [in persion] [Article]

13. Wikler MA. Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement. Wayne: Clinical and Laboratory Standards Institute. 2011; 31(1).

14. Naghoni A, Ranjbar R, Tabaraie B, Farshad S, Owlia P, Safiri Z, Mammina C. High prevalence of integron-mediated resistance in clinical isolates of Salmonella enterica. Jpn J Infect Dis 2010; 63(6): 417-21. [in persion] [PubMed]

15. Emaddi Chashni S.H, Hassanzadeh M, Bozorgmehri Fard M.H, Mirzaei S. Characterization of the Salmonella Isolates from Backyard Chickens in North of Iran, by Serotyping, Multiplex PCR and Antibiotic Resistance Analysis. RVSI 2009; 64(2): 77-83. [in persion] [Article]

16. Jin Y, Ling JM. Prevalence of integrons in antibiotic-resistant Salmonella spp. in Hong Kong. Jpn J Infect Dis 2009; 62(6): 432-439. [PubMed]

17. Molla B, Miko A, Pries K, Hildebrandt G, Kleer J, Schroeter A, Helmuth R. Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia. Acta Trop 2007; 103(2): 142-149. [PubMed]

18. Dahshan H, Chuma T, Shahada F, Akiba M, Fujimoto H, Akasaka K, Kamimura Y. Characterization of antibiotic resistance and the emergence of AmpC-producing Salmonella Infantis from pigs. J Vet Med Sci 2010; 72(11): 1437-1442. [PubMed]

19. Peymani A, Naserpour Farivar T, Rahimi H, Ranjbar M, Najafipour R. Frequency of class I integron among multidrug resistant Pseudomonas aeruginosa isolates from the selected hospitals in Qazvin and Tehran, Iran. Qom Univ Med Sci J 2014; 8(3): 61-69. [in persion] [Article]

20. Rahmani M, Peighambari SM, Svendsen CA, Cavaco LM, Agersø Y, Hendriksen RS. Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Vet Res 2013; 9(66): 163-171. [in persion] [Article]

21. Yan H, Li L, Alam MJ, Shinoda S, Miyoshi S, Shi L. Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China. Int J Food Microbiol 2010; 143(3): 230–234. [PubMed]

22. Benacer D, Thong KL, Watanabe H, Puthucheary SD. Characterization of drug resistant Salmonella enterica serotype Typhimurium by antibiograms, plasmids, integrons, resistance genes and PFGE. J Microbiol Biotechnol 2010; 20(6): 1042-1052. [PubMed]

23. Krauland MG, Marsh JW, Paterson DL, Harrison LH. Integron-mediated Multidrug Resistance in a Global Collection of Nontyphoidal Salmonella enterica Isolates. Emerg Infect Dis 2009; 15(3):388-96. [PubMed]