year 4, Issue 1 And 2 (9-2010)                   Iran J Med Microbiol 2010, 4(1 And 2): 9-16 | Back to browse issues page

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Oskoui M, Nobari S, Rahmati Ghezelgeh F, Shaghaghi B, Amirmozafari N. Molecular characterization of PBP2b in clinical isolates of Streptococcus pneumoniae. Iran J Med Microbiol 2010; 4 (1 and 2) :9-16
URL: http://ijmm.ir/article-1-54-en.html
1- Department of Microbiology, Pasteur Institute of Iran , oskoui1@yahoo.com
2- Department of Microbiology, Pasteur Institute of Iran
3- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences
Abstract:   (15189 Views)
Background and Objectives: Streptococcus pneumoniae remains one of the most important bacterial pathogens associated with pneumonia, meningitis, sinusitis, and otitis media. β-Lactam resistance in clinical pneumococci is mediated by altered PBPs, specifically PBP2b. The purpose of this study was to determine the rates of penicillin and other antibiotics resistance in clinical strains of Streptococcus pneumoniae which were isolated from patients in Tehran and to analyze possible mutations in pbp2b.
Material and Methods: A total of 54 S. pneumoniae strains were isolated from a number of different clinical centers in Tehran (2000-2008) which included Imam Khomeini hospital, Hazrat Rasoul hospital, Sina hospital, Shohada Tajrish hospital, Ali Asger hospital, Children Health Center, and Bahar pathobiology laboratory. After biochemical testing of all the 54 S. pneumoniae isolates, susceptibility testings were done by disc diffusion method for oxacillin, erythromycin, cefotaxime, trimethoprim /sulfamethoxazole, ampicillin, vancomycin and tetracycline. MIC values were determined by broth microdilution method for penicillin. Pbp2b gene was amplified by PCR and sequenced.
Results: From all the 54 isolates, 44.4% (24 isolates) were penicillin intermediate, 25.9% (14 isolates) were penicillin resistant, 51.9% (28 isolates) were ‎ trimethoprim /sulfamethoxazole resistant, 16.6% (9 isolates) were erythromycin resistant, 3.7% (2 isolates) were ‎cefotaxime resistant, and 18.51% (10 isolates) were tetracycline resistantَ. All of the penicillin resistant and most of the intermediate isolates carried mutations in the catalytic region of the in pbp2b gene.
Conclusion: Our results showed that penicillin resistance in Streptococcus pneumoniae is widespread. However, the prevalence of multiple resistant strains revealed a crisis in treatment of pneumococcal infections. Our investigation demonstrates that alterations in PBP2b tended to parallel with reduced susceptibility to penicillin. We have shown that susceptibility of Streptococcus pneumoniae to β-Lactam antibiotics can generally be estimated by determining alterations in pbp2b gene.
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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10

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