O-serogrouping of Escherichia coli strains isolated from patients with Urinary tract infection by using PCR method

Goudarzi, Vahid; Mirzaee, Mohsen; Ranjbar, Reza

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Goudarzi V, Mirzaee M, Ranjbar R. O-serogrouping of Escherichia coli strains isolated from patients with Urinary tract infection by using PCR method. Iran J Med Microbiol 2017; 10 (6) :1-8
URL: http://ijmm.ir/article-1-527-en.html

O-serogrouping of Escherichia coli strains isolated from patients with Urinary tract infection by using PCR method

Vahid Goudarzi¹

, Mohsen Mirzaee

², Reza Ranjbar³

1- Department of Cell and Molecular Biology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
2- Department of Medical Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran , mirzaei.iaub@gmail.com
3- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract: (11857 Views)

Background and Aim: Urinary tract infection (UTI) is one of the most common infections, and Escherichia coli is so far the most common causative agent of this disease. The O antigen, consisting of many repeats of an oligosaccharide unit, is part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria. The variability of the O antigen provides the major basis for serological schemes of Gram-negative bacteria. The aim of this study was to investigate the prevalence of O-serogroups among E. coli isolated from patients with UTI.

Materials and Methods: During three months, 210 patients with urinary tract infection, referred to three hospitals of Tehran, Sanandaj and Boroujerd. E. coli isolates were identified using standard methods then serogrouping was performed by using PCR method.

Results: A total of 150 E. coli strains were isolated from the urine samples. The most common types of O antigens were O1 (13.33%), O16 (8%), O8 (5.33%) and O21 (4%). There was no positive isolate for O22 and O83 genes.

Conclusions: The present study revealed that serogroup O1 was isolated with maximum frequency followed by O16, O8 and O21, respectively. In UPEC, the O-serogroups can be related to the virulence factor profile of each strain. Further studies from other parts of Iran and on other serogroup are recommended.

Keywords: Escherichia coli, Urinary tract infection, O-Antigen

Full-Text [PDF 608 kb] (3843 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2016/01/30 | Accepted: 2016/04/17 | ePublished: 2016/10/17

References

1. Schaechter M, Engleberg NC, Dirita VJ, Dermody TS. Schaechter's mechanisms of microbial disease. Pathophysiology of infection disease. 2nd ed. Lippincott Williams & Wilkins; 2012. p. 643-650. [Article]

2. Hamid-Farahani R, Tajik A, Noorifard M, Keshavarz A. Antibiotic resistance pattern of E.coli isolated from urine culture in 660 Army clinical laboratory center in Tehran 2008. J Army Univ Med Sci 2012; 10 (1): 45-49. [in Persian] [Article]

3. Wilson BA, Salyers AA, Whitt DD, Winkler ME. Bacterial pathogenesis: a molecular approach. 2nd ed. ASM Press; 2011. p. 823-832.

4. Sarkar S, Ulett GC, Totsika M, Phan M-D, Schembri MA. Role of Capsule and O Antigen in the Virulence of Uropathogenic Escherichia coli. PloS one 2014; 9(4): e94786. [Article] [PubMed]

5. Kalynych S, Yao D, Magee J, Cygler M. Structural characteriza‌tion of closely related O-antigen lipopolysaccharide (LPS) chain length regulators. J Biol Chem 2012; 287(19):15696–705. [Article] [PubMed]

6. Wang Q, Perepelov AV, Beutin L, Senchenkova SN, Xu Y, Shashkov AS and et al. Structural and genetic characterization of the Escherichia coli O180 O antigen and identification of a UDP-GlcNAc 6-dehydrogenase. Glycobiology 2012; 22(10):1321–31. [Article] [PubMed]

7. Debroy C, Roberts E, Valadez AM, Dudley EG, Cutter CN. Detection of Shiga toxin–producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster. Foodborne Pathog Dis 2011; 8(5):651-2. [Article] [PubMed]

8. Liu Y, Yan X, Debroy C, Fratamico PM, Needleman DS, Li RW, Wang W, Losada L, Brinkac L, Radune D, Toro M. Escherichia coli O-antigen gene clusters of serogroups O62, O68, O131, O140, O142, and O163: DNA sequences and similarity between O62 and O68, and PCR-based serogrouping. Biosensors 2015; 5(1):51-68. [PubMed]

9. DebRoy C, Roberts E, Fratamico PM. Detection of O antigens in Escherichia coli. Anim Health Res Rev 201; 12 (2): 169–185. [PubMed]

10. Momtaz H, Karimian A, Madani M, Safarpoor Dehkordi F, Ranjbar R, Sarshar M, Souod N. Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties. Ann Clin Microbiol Antimicrob 2013; 12 (8): e 23627669. [PubMed]

11. Yun KW, Kim DS, Kim W, Lim IS. Molecular typing of uropathogenic Escherichia coli isolated from Korean children with urinary tract infection. Korean J Pediatr 2015; 58(1): 20-7. [PubMed]

12. Molina-López J, Aparicio-Ozores G, Ribas-Aparicio RM, Gavilanes-Parra S, Chávez-Berrocal ME, Hernández-Castro R, Manjarrez-Hernández HÁ. Drug resistance, serotypes, and phylogenetic groups among uropathogenic Escherichia coli including O25-ST131 in Mexico City. J Infect Dev Ctries 2011; 5(12):840-9. [Article] [PubMed]

13. Li D, Liu B, Chen M, Guo D, Guo X, Liu F, Feng L, Wang L. A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections. J Microbiol Methods 2010; 82(1):71-7 [PubMed]

14. Kulkarni R, Dhakal BK, Slechta ES, Kurtz Z, Mulvey MA, Thanassi DG. Roles of putative type II secretion and type IV pilus systems in the virulence of uropathogenic Escherichia coli. PLoS One 2009; 4(3): e4752. [PubMed]

15. Nielubowicz GR and Harry M. Host–pathogen interactions in urinary tract infection. Nat Rev Urol 2010; 7 (8): 430-441. [PubMed]

16. Karimian A, Momtaz H, Madani M. Detection of uropathogenic Escherichia coli virulence factors in patients with urinary tract infections in Iran. Afr J Microbiol Res 2012; 6(39): 6811-6. [Article]

17. Kudinha T, Kong F, Johnson JR, Andrew SD, Anderson P, Gilbert GL. Multiplex PCR-based reverse line blot assay for simultaneous detection of 22 virulence genes in uropathogenic Escherichia coli. Appl Environ Microbiol 2012; 78(4): 1198–202. [PubMed]

18. Asadi S, Kargar M, Solhjoo K, Najafi A, and Ghorbani-Dalini S. The association of virulence determinants of uropathogenic Escherichia coli with antibiotic resistance. Jundishapur J Microbiol 2014; 7 (7): e9936. [in Persian] [PubMed]

19. Rashki A and Hussein AA. O-serotyping of Escherichia coli Strains isolated from Patients with Urinary Tract Infection in Southeast of Iran. Int J Enteric Pathog 2014; 2(4): e20968 [Article]

20. Wang Q, Perepelov AV, Beutin L, Senchenkova SN, Xu Y, Shashkov AS, et al. Structural and genetic characterization of the Escherichia coli O180 O antigen and identification of a UDP-GlcNAc 6-dehydrogenase.Glycobiolog 2012; 22(10):1321-31. [Article]

21. Shweta S, Nirmaljit K, Shalini M, Preeti M, Wasim A and Charoo H. Serotyping and Antimicrobial Susceptibility Pattern of Escherichia coli Isolates from Urinary Tract Infections in Pediatric Population in a Tertiary Care Hospital. J Pathog 2016; e2548517. [PubMed]

22. Emamghoraishi F, Farshad S, Kalani M. Relationship between O serotype and virulent genes in Escherichia coli causing urinary tract infections. Iran J Kidney Dis 2011; 5(4):234-7. [in Persian] [PubMed]

23. Dormanesh B, Safarpoor F, Hosseini S, Momtaz H, Mirnejad R, Hoseini MJ, Yahaghi E. Virulence factors and O-serogroups profiles of uropathogenic Escherichia coli isolated from Iranian pediatric patients. Iran Red Crescent Med J 2014; 16 (2). e14627. [in Persian] [PubMed]

24. Issazadeh K, Naghibi SN, Khoshkholgh-Pahlaviani MM. Drug Resistance and Serotyping of Uropathogenic Escherichia coli among Patients with Urinary Tract Infection in Rasht, Iran. Zahedan J Res Med Sci 2015; 17 (6): 1-5. [in Persian] [Article] [Google Scholar]