year 10, Issue 5 (November - December 2016)
Iran J Med Microbiol 2016, 10(5): 31-37 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
MohammadAlipour Z, Asadpour L, Ranji N. Fluoroquinolone resistance and mutation in gyrA gene in clinical isolates of Klebsiella pnemoniae. Iran J Med Microbiol 2016; 10 (5) :31-37
URL: http://ijmm.ir/article-1-501-en.html
URL: http://ijmm.ir/article-1-501-en.html
1- Department of Microbiology, Faculty of Science, Islamic Azad University of Rasht, Rasht, Iran
2- Department of Microbiology, Faculty of Science, Islamic Azad University of Rasht, Rasht, Iran ,Asadpour@iaurasht.ac.ir
3- Department of Genetic, Faculty of Science, Islamic Azad University of Rasht, Rasht, Iran
2- Department of Microbiology, Faculty of Science, Islamic Azad University of Rasht, Rasht, Iran ,
3- Department of Genetic, Faculty of Science, Islamic Azad University of Rasht, Rasht, Iran
Abstract: (10146 Views)
Background and Aim: Fluoroquinolone resistance has been mainly associated with mutation in gyr and par genes bacteria and specific amino acids alteration in gram negative bacteria. In the present study, urinary tract infection isolates of Klebsiella pneumonia were investigated for fluoroquinolone resistance and mutation patterns of gyrA gene in ciprofloxacin resistant isolates.
Materials and Methods: In 2014, Susceptibility to fluoroquinolones in 46 isolates of
K. pneumoniae was tested by the disc diffusion method whereas broth macrodilution method was used to investigate ciprofloxacin minimum inhibitory concentration. Mutations in the quinolone resistance-determining regions (QRDR) was investigated in chromosomal gyrA gene in quinolone-resistant K. pnemoniae isolates, by PCR amplification of gyrA gene and sequencing.
Results: Out of 46 tested bacteria, ten (21.7%) isolates recognized as fluoroquinolones resistant. Minimum inhibitory concentration of ciprofloxacin for resistant isolates ranged between 64 to 2048 µg/mL. In gyrA gene sequence analyses, 8 (80%) isolates had an amino acid substitution. In six isolates mutation occurred in Asp87 that amino acid alters with Alanine and asparagine. Also Ser83Ile substitution was found in one isolate.
Conclusions: The obtained results demonstrated that gyrA mutation is one of the most important mechanisms of resistance to ciprofloxacin in clinical isolates of K. pneumonia in Rasht.
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2015/11/2 | Accepted: 2016/09/4 | ePublished: 2016/10/17
Received: 2015/11/2 | Accepted: 2016/09/4 | ePublished: 2016/10/17
References
1. Hashemi A, Fallah F, Taherpour A, Goudarzi H, Erfanimanesh S, Taki E. Evaluation of genetic pattern and determination of oqxA gene expression levels among clinical isolates of Klebsiella Pneumoniae strains. J Mazandaran Univ Med Sci 2014;24(119):48-61. [in persion] [Article]
2. Won SY, Munoz-Price LS, Lolans K, Hota B, Weinstein RA, Hayden MK. Emergence and rapid regional spread of Klebsiella pneumoniae carbapenemase producing Enterobacteriaceae. Clin Infec Dis 2011;53(6):532-40. [PubMed]
3. Patel G, Bonomo RA. Status report on carbapenemases: challenges and prospects. Expert Rev Anti Infect Ther 2011;1:9(5):555-70. [PubMed]
4. Kim MH, Lee HJ, Park KS, Suh JT. Molecular characteristics of extended spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae and the prevalence of qnr in extended spectrum β-lactamase isolates in a tertiary care hospital in Korea. Yonsei Med.J 2010;51(5):768-74. [PubMed]
5. Zibaei M, Firoozeh F. Detection of qnrA gene among quinolone-resistant Escherichia coli isolated from urinary tract infections in Khorram Abad during 2011-2012. Feyz 2013;17(5):488-94. [in persion] [Article]
6. Fazeli H, Vakili B, Khorvash F, Shoaei P, Kariminik A, Yaran M, et al. Identification of mutation in gyrA gene obtained from quinolone resistant clinical isolates of Acintobacter baumani. Journal of Microbial World 2014;7(2):109-17. [in persion] [Article]
7. Gallini A, Degris E, Desplas M, Bourrel R, Archambaud M, Montastruc J-L, et al. Influence of fluoroquinolone consumption in inpatients and outpatients on ciprofloxacin-resistant Escherichia coli in a university hospital. J Antimicrob Chemother 2010;65(12):2650-57. [PubMed]
8. Rushdy AA, Mabrouk MI, Abu-Sef FAH, Kheiralla ZH, Abdel-All SM, Saleh NM. Contribution of different mechanisms to the resistance to fluoroquinolones in clinical isolates of Salmonella enterica. Braz j infect dis 2013;17(4):431-7. [PubMed]
9. Vernaz N, Huttner B, Muscionico D, Salomon JL, Bonnabry P, Lopez-Lozano JM, et al. Modelling the impact of antibiotic use on antibiotic-resistant Escherichia coli using population-based data from a large hospital and its surrounding community. J Antimicrob Chemother dkq525;1-8. [PubMed]
10. Cremet L, Caroff N, Dauvergne S, Reynaud A, Lepelletier D, Corvec S. Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. Pathol Biol 2011;59(3):151-6. [PubMed]
11. Tumbarello M, Trecarichi EM, Bassetti M, De Rosa FG, Spanu T, Di Meco E, et al. Identifying patients harboring ESBL-Producing Enterobacteriaceae on hospital admission: Derivation and validation of a scoring system. Antimicrob Agents Chemother 2011;55(7):3485-90. [PubMed]
12. Koo SH, Kwon KC, Cho HH, Sung JY. Genetic basis of multidrug-resistant Acinetobacter baumannii clinical isolates from three university hospitals in Chungcheong Province, Korea. Korean J Lab Med 2010;30(5):498-506. [PubMed]
13. Al-Marzooq F, Mohd Yusof MY, Tay ST. Molecular analysis of ciprofloxacin resistance mechanisms in malaysian ESBL-producing Klebsiella pneumoniae isolates and development of mismatch amplification mutation assays (MAMA) for rapid detection of gyrA and parC mutations. Biomed Res Int 2014;601-30. [PubMed]
14. Jaktaji RP, Mohiti E. Study of mutations in the DNA gyrase gyrA gene of Escherichia coli. Iran J Pharm Res 2010;9(1):43-8. [in persion] [PubMed]
15. Norouzi A, Azizi O, Hosseini H, Shakibaie S. Amino acid substitution mutations analysis of gyrA and parC genes in clonal lineage of Klebsiella pneumoniae conferring high-level quinolone resistance. Journal of Medical Microbiology and Infectious Diseases (JoMMID) 2014;2(3):109-17. [in persion] [Article]
16. Gupta V, Yadav A, Joshi R. Antibiotic resistance pattern in uropathogens. Indian J Med Microbiol 2002;20(2):96-8.
17. Song Y, Liu C, McTeague M, Finegold SM. 16S ribosomal DNA sequence-based analysis of clinically significant gram-positive anaerobic cocci. J Clin Microbiol 2003;41(4):1363-9. [PubMed]
18. Brisse S, Verhoef J. Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and parC genes sequencing and automated ribotyping. Int J Syst Evol Microbiol 2001;51(3):915-24. [PubMed]
19. Fu Y, Zhang W, Wang H, Zhao S, Chen Y, Meng F, et al. Specific patterns of gyrA mutations determine the resistance difference to ciprofloxacin and levofloxacin in Klebsiella pneumoniae and Escherichia coli. BMC Infect Dis 2013;13(1):8-15. [Article]
20. Fu Y, Guo L, Xu Y, Zhang W, Gu J, Xu J, et al. Alteration of gyrA amino acid required for ciprofloxacin resistance in Klebsiella pneumoniae isolates in China. Antimicrob Agents Chemother 2008;52(8):2980-3. [PubMed]
21. Vila A, Cassata A, Pagella H, Amadio C, Yeh K-M, Chang F-Y, et al. Appearance of Klebsiella pneumoniae liver abscess syndrome in Argentina: case report and review of molecular mechanisms of pathogenesis. Open Microbiol J 2011;5:107-113. [PubMed]
22. Erac B, Gill A, Amyes S, Gulay Z. Mutations of gyrA in ciprofloxacin resistant Escherichia coli strains. Mikrobiyol Bul 2002;37(2-3):125-30. [PubMed]
23. Minarini LA, Darini ALC. Mutations in the quinolone resistance-determining regions of gyrA and parC in Enterobacteriaceae isolates from Brazil. Braz J Microbiol 2012;43(4):1309-14. [PubMed]
24. Luo Y, Yang J, Zhang Y, Ye L, Wang L, Guo L. Prevalence of β-lactamases and 16S rRNA methylase genes amongst clinical Klebsiella pneumoniae isolates carrying plasmid-mediated quinolone resistance determinants. Int J Antimicrob Agents 2011;37(4):352-5. [PubMed]
25. Singh M, Jadaun G, Ramdas KS, Chauhan V, Mishra R, Gupta K, et al. Effect of efflux pump inhibitors on drug susceptibility of ofloxacin resistant Mycobacterium tuberculosis isolates. Indian J Med Res 2011;133(5):535-540. [PubMed]
26. Wang D, Wang H, Qi Y, Liang Y, Zhang J, Yu L. Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac (6')-Ib-cr, GyrA and CTX-M-22 genes. Folia Histochem Cytobiol 2012;50(1):68-74.
27. Bouchakour M, Zerouali K, Claude JDPG, Amarouch H, El Mdaghri N, Courvalin P, et al. Plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing Enterobacteriaceae in Morocco. J Infect Dev Ctries 2010;4(12):779-803. [PubMed]
28. Corkill JE, Anson JJ, Hart CA. High prevalence of the plasmid-mediated quinolone resistance determinant qnrA in multidrug-resistant Enterobacteriaceae from blood cultures in Liverpool, UK. J Antimicrob Chemother 2005;56(6):1115-7. [PubMed]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
