Comparison of bax, ibpAB and cspH genes in Escherichia coli isolates from urine and fecal samples

Abdi, Elham; Ahmadi, Amjad; Azizaian, Khalil; Talebi Aghdam, Roya; Taherpour, Arezou

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Comparison of bax, ibpAB and cspH genes in Escherichia coli isolates from urine and fecal samples

Elham Abdi¹

, Amjad Ahmadi²

, Khalil Azizaian¹

, Roya Talebi Aghdam¹

, Arezou Taherpour³

1- Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Health Metrics and Evaluation Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran , rezaeeit93@gmail.com

Abstract: (67 Views)

Background and Aim: The escalating antimicrobial resistance (AMR) in Escherichia coli (E. coli) and its rapid global dissemination present formidable challenges in the clinical management of infections. This study aimed to quantify the prevalence and relative expression levels of the bax, ibpA, ibpB, and cspH genes in E. coli isolates obtained from matched urine and fecal samples of patients diagnosed with urinary tract infections (UTIs) in Sanandaj, Iran, during the 2023-2024 period. Materials and Methods: In this cross-sectional investigation, 50 E. coli isolates (25 urinary and 25 fecal) were collected from of patients diagnosed with urinary tract infections (UTIs) in Sanandaj, Iran between 2023 and 2024( A fecal sample was taken from the same person with an E. coli positive urinary tract infection ). Molecular characterization was performed via PCR to determine gene frequency, and relative expression levels of the bax, ibpA, ibpB, and cspH genes were analyzed using quantitative Real-Time PCR (qPCR. Results: Analysis of relative expression levels showed that the expression of all 4 genes was significantly higher in urinary isolates compared to fecal isolates, but it is noteworthy that there was only a significant correlation between the relative expression levels of cspH(p-value:0.02) and ibpA(p-value:0.00) genes in urinary and fecal samples. No significant difference in biofilm formation capacity was observed between the two groups (P > 0.05). Conclusion: This investigation identifies a significant differential expression of the bax, ibpA, ibpB, and cspH genes between fecal and urinary E. coli isolates, whereas biofilm-forming capacity remained consistent across sources.

Keywords: urinary tract infection, PCR, E. coli, bax, IbpA