year 8, Issue 3 (Fall 2014)                   Iran J Med Microbiol 2014, 8(3): 1-7 | Back to browse issues page

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Salimi chirani A, Dabiri H, Nikokar I, Ebrahim pour komleh M, Dousdar F, Goudarzi H, et al . Evaluation of type IV pilin sub groups in Pseudomonas aeruginosa isolated from environmental samples, cystic fibrosis and burn patients. Iran J Med Microbiol 2014; 8 (3) :1-7
URL: http://ijmm.ir/article-1-290-en.html
1- Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , Hodabiri@gmail.com
Abstract:   (15590 Views)

Background and Aim: Type IV pilin molecule in Pseudomonas aeruginosa with wide functions, has important role in the diversity of the bacterial genome.  According to the latest classification, Type IV pilin is divided to two main subtypes IVa, IVb. The major subunit encoding Type IV pilin subtypes IVa, IVb is pilA and pilS2 genes respectively which serve as a marker for the detection of the Type IV pilin subtypes. The purpose of the current study was to evaluate the frequency of pilA and pilS2 gene in cystic fibrosis, burns, and environmental isolates.

Materials and Methods: The samples for P.aeruginosa were collected from cystic fibrosis, burns, and environment wastewater during the April 2013 to December 2013. Samples were cultured and identified using microbial and biochemical methods. DNA of isolates was extracted by commercial kit. PCR was performed using specific primers. Statistical analysis was done using SPSS 17.0 software.

Results: A total of 100 P.aeruginosa isolates were collected 30 cystic fibrosis, 30 burn, 40 environmental. The prevalence of the  pilA gene were 63.3% , 56.7% and 45/2%  for cystic fibrosis, for burns  and environmental strains . The PilS2 gene frequency was 57.5% of the environmental isolates, 67.5% of cystic fibrosis strains and 73.3 % of the isolates were burned.

Conclusions: Our study showed that the frequency of P.aeruginosa T4P major pilin subunits in our P. aeruginosa population was high. The incidence of pilS2 gene was greater than pilA and was more frequent in Pseudomonas aeruginosa isolated from hospitalized burn patients.

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2014/06/15 | Accepted: 2014/09/15 | ePublished: 2014/11/19

References
1. Mulcahy LR, Isabella VM, Lewis K. Pseudomonas aeruginosa Biofilms in Disease. Microb Ecol. 2014;68(1):1-12. [DOI:10.1007/s00248-013-0297-x] [PMID] [PMCID]
2. Lyczak JB, Cannon CL, Pier GB. Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect / Institut Pasteur. 2000;2(9):1051-60. [DOI:10.1016/S1286-4579(00)01259-4]
3. Kelsey M. Pseudomonas in augmented care: should we worry? J Antimicrob Chemother. 2013;68(12):2697-700. [DOI:10.1093/jac/dkt288] [PMID]
4. Finnan S, Morrissey JP, O'Gara F, Boyd EF. Genome Diversity of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients and the Hospital Environment. J Clin Microbiol. 2004;42(12):5783-92. [DOI:10.1128/JCM.42.12.5783-5792.2004] [PMID] [PMCID]
5. Craig L, Pique ME, Tainer JA. Type IV pilus structure and bacterial pathogenicity. Nat Rev Microbiol. 2004;2(5):363-78. [DOI:10.1038/nrmicro885] [PMID]
6. Pelicic V. Type IV pili: e pluribus unum? Mol Microbiol. 2008;68(4):827-37. [DOI:10.1111/j.1365-2958.2008.06197.x] [PMID]
7. Stanley PM. Factors affecting the irreversible attachment of Pseudomonas aeruginosa to stainless steel. Can J Microbiol. 1983;29(11):1493-9. [DOI:10.1139/m83-230] [PMID]
8. Stone JH, Gabriel MM, Ahearn DG. Adherence of Pseudomonas aeruginosa to inanimate polymers including biomaterials. J Ind Microbiol Biotechnol. 1999;23(1):713-7. [DOI:10.1038/sj.jim.2900686] [PMID]
9. Carter MQ, Chen J, Lory S. The Pseudomonas aeruginosa pathogenicity island PAPI-1 is transferred via a novel type IV pilus. J Bacteriol. 2010;192(13):3249-58. [DOI:10.1128/JB.00041-10] [PMID] [PMCID]
10. Burrows LL. Pseudomonas aeruginosa twitching motility: type IV pili in action. Annu Rev Microbiol. 2012;66:493-520. [DOI:10.1146/annurev-micro-092611-150055] [PMID]
11. Lee KK, Sheth HB, Wong WY, Sherburne R, Paranchych W, Hodges RS, et al. The binding of Pseudomonas aeruginosa pili to glycosphingolipids is a tip-associated event involving the C-terminal region of the structural pilin subunit. Mol Microbiol. 1994;11(4):705-13. [DOI:10.1111/j.1365-2958.1994.tb00348.x] [PMID]
12. Morales-Espinosa R, Soberon-Chavez G, Delgado-Sapien G, Sandner-Miranda L, Mendez JL, Gonzalez-Valencia G, et al. Genetic and phenotypic characterization of a Pseudomonas aeruginosa population with high frequency of genomic islands. PloS one. 2012;7(5):e37459. [DOI:10.1371/journal.pone.0037459] [PMID] [PMCID]
13. Ramsey MM, Whiteley M. Pseudomonas aeruginosa attachment and biofilm development in dynamic environments. Mol Microbiol. 2004;53(4):1075-87. [DOI:10.1111/j.1365-2958.2004.04181.x] [PMID]
14. Schwartz T, Volkmann H, Kirchen S, Kohnen W, Schon-Holz K, Jansen B, et al. Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates. FEMS Microbiol Ecol. 2006;57(1):158-67. [DOI:10.1111/j.1574-6941.2006.00100.x] [PMID]
15. Kus JV, Tullis E, Cvitkovitch DG, Burrows LL. Significant differences in type IV pilin allele distribution among Pseudomonas aeruginosa isolates from cystic fibrosis (CF) versus non-CF patients. Microbiology. 2004;150(5):1315-26. [DOI:10.1099/mic.0.26822-0] [PMID]
16. Castric PA, Deal CD. Differentiation of Pseudomonas aeruginosa pili based on sequence and B-cell epitope analyses. Infect Immun. 1994;62(2):371-6. [DOI:10.1128/IAI.62.2.371-376.1994] [PMID]
17. Spangenberg C, Fislage R, Sierralta W, Tummler B, Romling U. Comparison of type IV-pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence. FEMS Microbiol Lett. 1995;125(2-3):265-73. [DOI:10.1111/j.1574-6968.1995.tb07367.x] [PMID]
18. Klockgether J, Wurdemann D, Reva O, Wiehlmann L, Tummler B. Diversity of the abundant pKLC102/PAGI-2 family of genomic islands in Pseudomonas aeruginosa. J Bacteriol. 2007;189(6):2443-59. [DOI:10.1128/JB.01688-06] [PMID] [PMCID]

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