Typing and Evaluation of the Genetic Relatedness of Listeria monocytogenes Strains Isolated from Food Samples by the Multiple-Locus Variable number Tandem Repeat Analysis (MLVA)

Sadeghi kalani, Behrooz; Irajian, Gholam reza; Bahador, Abas; Pournajaf, Abazar; Dianat moghadam, Hassan; Neghabi, Mahdieh; Solimany Jelodar, Rahim

year 8, Issue 4 (Winter 2015) Iran J Med Microbiol 2015, 8(4): 13-19 | Back to browse issues page

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Sadeghi kalani B, Irajian G R, Bahador A, Pournajaf A, Dianat moghadam H, Neghabi M et al . Typing and Evaluation of the Genetic Relatedness of Listeria monocytogenes Strains Isolated from Food Samples by the Multiple-Locus Variable number Tandem Repeat Analysis (MLVA). Iran J Med Microbiol 2015; 8 (4) :13-19
URL: http://ijmm.ir/article-1-272-en.html

Typing and Evaluation of the Genetic Relatedness of Listeria monocytogenes Strains Isolated from Food Samples by the Multiple-Locus Variable number Tandem Repeat Analysis (MLVA)

Behrooz Sadeghi kalani¹

, Gholam reza Irajian

², Abas Bahador¹

, Abazar Pournajaf¹

, Hassan Dianat moghadam³

, Mahdieh Neghabi¹

, Rahim Solimany Jelodar⁴

1- Faculty of Medicine
2- 2. Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. , Dr.irajian@gmail.com
3- School of Medicine nano Technologies
4- Department of Virology, Faculty of Medicine, Ahvaz jundishapour University of Medical Sciences, Iran.

Abstract: (14423 Views)

Background and Aim:Listeria monocytogenes cause listeriosis and fatal infections in humans. The aim of this study was typing and evaluation of the genetic relatedness of L. monocytogenes strains from food samples using MLVA technique.

Materials and Methods: 317 food samples were collected from 2009 to 2013 in Tehran,Iran. After final diagnosis of L. monocytogenes DNA was extracted to perform of MLVA technique, and also PCR products were analyzed by Gene Tools software. The number of tandem repeats was determined by using special equation for each selected locus. Also typing of strains was done.

Results: 24 samples of 317 food samples were positive for L. monocytogenes using standard laboratory techniques. A total 13 different types were determined by MLVA technique that type 2 and type 3 were the most abundant types by 6 and 4 strains, respectively.

Conclusions: The results of this study showed the presence of L. monocytogenes in dairy products and meat samples, therefore all people, especially pregnant women should observe health tips when using these products. The results of typing showed that L. monocytogenes strains from different sources can have the same origin. MLVA technique is easy with high accuracy and this method can be used in typing and evaluation of the genetic relatedness of L. monocytogenes for determination the source of contamination.

Keywords: Listeria monocytogenes, Dairy and meat samples, MLVA

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2014/04/27 | Accepted: 2014/11/21 | ePublished: 2014/11/23